Expression Vectors
The Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO® Expression Vectors (Figure 1) allow you to rapidly clone your gene and fuse it to the widely used and well characterized Fluorescent Proteins (FPs) from the jellyfish Aequorea victoria (1, 2). These powerful TOPO® cloning vectors contain the Emerald Green Fluorescent Protein (EmGFP) or the Yellow Fluorescent Protein (YFP) for simple, non-invasive detection of recombinant protein (Figure 2). Both FPs have been humanized for optimal mammalian expression (3). The Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO® Expression Vectors offer: • Top...
Catalog: K359-20
Size: 20 reactions
Size: 20 reactions
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 11809-019
Size: 6 µg
Size: 6 µg
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 12301-016
Size: 6 µg
Size: 6 µg
The pLenti6.2⁄V5-DEST™ Gateway® Vector is a Gateway®-adapted ViraPower™ II lentiviral expression vector for lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of the target gene and the PGK promoter for driving long-term, persistent expression of the Blasticidin stable selection marker. Advantages • Stable expression • Long-term experiments • High-throughput screening • Accurate titer of functional virus (using Blasticidin method) • Flexible and versatile Gateway® recombination cloning t...
Catalog: V368-20
Size: 6 µg
Size: 6 µg
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 12288-015
Size: 6 µg
Size: 6 µg
The pcDNA™3.1 Directional TOPO® Expression Kit uses linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO® for five-minute directional cloning and subsequent high-level expression. Directional Cloning technology facilitates expression experiments because: A proofreading enzyme is used for fewer errors in cloned genes Greater than 90% of the clones are in the correct orientation for expression, reducing time spent colony screening for clone orientation In addition, pcDNA3.1D/V5-His-TOPO® provides strong expression levels from the CMV promoter and the option of a C-terminal V5-His fusion t...
Catalog: K4900-40
Size: 40 reactions
Size: 40 reactions
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 11803-012
Size: 6 µg
Size: 6 µg
The ViraPower™ HiPerform™ T-REx™ Gateway® Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral, T-REx™ and Gateway® technologies to facilitate easy recombination-based cloning and lentiviral-based, regulated (Tetracycline-inducible), high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄ TO⁄ V5-DEST vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscr...
Catalog: A11141
Size: 20 reactions
Size: 20 reactions
The InsectSelect™ Glow Kit features the pIZT/V5-His vector for high-level expression of your gene of interest. This vector has the following features: The OpIE 2 promoter for constitutive expression The Zeocin™ resistance gene for rapid selection of stably transfected cell lines C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistance protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid...
Catalog: V8010-01
Size: 1 kit
Size: 1 kit
The DES® TOPO® TA Expression Kit offers one-step cloning of PCR products directly into an inducible DES® expression vector. The kit uses the linearized, topoisomerase I-activated pMT/V5-His-TOPO® vector for fast and easy cloning and subsequent high-level expression. Topoisomerase activation of this vector allows PCR products to be ligated in just 5 minutes on your bench top to produce >85%recombinants. The pMT/V5-His-TOPO® vector has all of the features of the pMT/V5-His vector to simplify protein expression, detection, and purification of recombinant proteins in Drosophila S2 cells.
Catalog: K4125-01
Size: 20 reactions
Size: 20 reactions
The Champion™ pET-DEST42 Gateway® destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3) E. coli. The Champion™ pET-DEST42 destination vector offers: Bacteriophage T7 lac promoter for high-level expression O operator sequences for lac repressor binding to ensure tighter regulation of transcription pBR322 ori for minimal basal expression C-terminal V5 and...
Catalog: 12276-010
Size: 6 µg
Size: 6 µg
The Baculovirus Expression System with Gateway® Technology is designed to create recombinant pFastBac™ plasmids containing the polyhedrin promoter. The Baculovirus Expression System with Gateway® Technology offers: Expression using the Bac-to-Bac® technology (1) Destination vectors carrying the polyhedrin promoter for production of native (pDEST™8), N-terminal histidine fusion (pDEST™10), and N-terminal GST fusion (pDEST™20) proteins pDEST™10 vector containing a TEV protease cleavage site for removal of the fusion tag after protein purification (2)
Catalog: 11827-011
Size: 20 reactions
Size: 20 reactions
pcDNA™FRT⁄TO vector is a 5.1 kb inducible expression vector designed for use with the Flp-In™ T-REx™ System. The control plasmid, pcDNA™5⁄FRT⁄TO⁄CAT, is also included for use as a positive control for transfection and expression in your Flp-In™ T-REx™ host cell line of choice. The pcDNA™5⁄FRT⁄TO vector contains the following elements: • A hybrid human cytomegalovirus (CMV)⁄TetO2 promoter for high-level, tetracycline-regulated expression of the gene of interest in a wide range of mammalian cells • Multiple cloning site with 10 unique restriction sites to facilitate cloning the gene of interest ...
Catalog: V6520-20
Size: 20 µg
Size: 20 µg
The ViraPower™ HiPerform™ Lentiviral FastTiter™ TOPO® Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral and TOPO® technologies to facilitate quick PCR-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti7.3/V5-TOPO® vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central ...
Catalog: K5320-00
Size: 1 kit
Size: 1 kit
The ViraPower™ Lentiviral Directional TOPO® Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. It combines Invitrogen’s ViraPower™ Lentiviral and Directional TOPO® technologies to facilitate quick PCR-based cloning and lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6⁄V5-D-TOPO® vector has the CMV promoter for driving constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells. Advantages • Lentivirus...
Catalog: K4950-00
Size: 1 kit
Size: 1 kit
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/GW/D-TOPO vector offers the following key features: •Cytomegalovirus (CMV) promoter for high-level expression •Adapted for Directional TOPO® Cloning, enabling you to use proofreading polymerases and to clone your PCR products in a specific orientation •Neomycin resistance gene for stable selection •C-terminal V5 tag for easy detection •Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli TOPO® Cloning Using restriction enzymes to clone your ge...
Catalog: K2440-20
Size: 20 reactions
Size: 20 reactions
pcDNA5⁄FRT vector is a 5.1 kb expression vector designed for use with the Flp-In™ System. This vector features: 1. CMV promoter for high level expression in mammalian cells; 2. ten unique restriction site for easy cloning; 3. FLP Recombination Target (FRT) site for Flp recombinase-mediated integration of the vector into the Flp-In™ host cell line and 4. Hygromycin resistance gene for selection of stable cell lines.
Catalog: V6010-20
Size: 20 µg
Size: 20 µg
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 11807-013
Size: 6 µg
Size: 6 µg
Blasticidin is a potent selection agent that allows selection of stable mammalian cell lines in as little as one week. The BsdCassette™ vectors simplify the construction of custom vectors that use Blasticidin resistance as a selectable marker. Each BsdCassette™ vector is designed for use in a specific host system and offers: A strong host promoter to drive expression of the Blasticidin resistance gene ( bsd )(Table 1) Two large polylinkers flanking the promoter and bsd gene to facilitate the construction of Blasticidin resistant vectors Each BsdCassette™ vector carries the synthetic EM-7 promo...
Catalog: V510-20
Size: 20 µg
Size: 20 µg
The Ambion® pMIR-REPORT™ miRNA Expression Reporter Vector System is for the measurement of miRNA expression in cells. It contains two mammalian expression vectors-—one for cloning miRNA targets and evaluating miRNA regulation, and the other for the normalization of transfection efficiency. Features of the pMIR-REPORT™ miRNA Expression Reporter Vector System: • Clone miRNA targets and evaluate miRNA regulation • Screen putative miRNA target sequences • Includes both reporter and control vectors The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell mea...
Catalog: AM5795M
Size: 1 kit w⁄ Manual
Size: 1 kit w⁄ Manual
Expression Vectors
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