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Champion™ pET101 Directional TOPO® Expression Kit with BL21 Star™ (DE3) One Shot® Chemically Competent E. coli

Category: Products & Services > Kits & Assays > Protein Expression Kits > Bacterial Expression Kits

The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Catalog: K101-01
Size: 20 reactions

Champion™ pET100 Directional TOPO® Expression Kit with BL21 Star™ (DE3) One Shot® Chemically Competent E. coli

Category: Products & Services > Kits & Assays > Protein Expression Kits > Bacterial Expression Kits

The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Catalog: K100-01
Size: 20 reactions

One Shot® BL21(DE3) Chemically Competent E. coli

Category: Products & Services > Competent Cells & Strains > Expression Competent Cells

BL21(DE3) E. coli are ideal for use with bacteriophage T7 promoter-based expression systems ( e.g. , pRSET, pCR¤T7, and pET). BL21(DE3) carry the lambda DE3 lysogen. Recombinant proteins that are non-toxic to E. coli are generally expressed at higher levels in BL21(DE3) cells than in BL21(DE3)pLysS or BL21(DE3)pLysE. However, the basal expression levels of heterologous genes are significantly higher in BL21(DE3) than in BL21(DE3)pLysS or BL21(DE3)pLysE.
Catalog: C6000-03
Size: 20 × 50 µl

BL21-AI™ One Shot® Chemically Competent E. coli

Category: Products & Services > Competent Cells & Strains > Expression Competent Cells

One Shot® BL21-AI™ E. coli are chemically competent cells designed for applications that require tight regulation and strong expression of toxic proteins from any T7 promoter-based expression systems. One Shot® BL21-AI™ Chemically Competent cells have a transformation efficiency of >1×10 8 cfu/ µg plasmid DNA. • Ideal for inducing expression of toxic protein • Gene expression is regulated by the addition of L-arabinose to culture • Suitable for use with any T7 promoter-based plasmids • Supplied in a convenient One Shot® format Excellent Expression of Toxic Recombinant Proteins One Shot® BL21-A...
Catalog: C6070-03
Size: 20 × 50 µl

One Shot® BL21(DE3)pLysE Chemically Competent E. coli

Category: Products & Services > Competent Cells & Strains > Expression Competent Cells

BL21(DE3)pLysE E. coli are ideal for use with T7 promoter-based expression vectors. Like BL21(DE3)pLysS E. coli , BL21(DE3)pLysE carry the lambda DE3 lysogen. In addition, BL21(DE3)pLysE cells contain the pLysE plasmid, which constitutively expresses T7 lysozyme. T7 lysozyme reduces the basal expression of target genes by inhibiting T7 RNA polymerase. The pLysE plasmid in BL21(DE3)pLysE expresses higher levels of T7 lysozyme than the pLysS plasmid in BL21(DE3)pLysS. The BL21(DE3)pLysE strain provides tighter control of T7 RNA polymerase, which is necessary when the recombinant protein to be ex...
Catalog: C6565-03
Size: 20 × 50 µl

One Shot® BL21 Star™ (DE3) Chemically Competent E. coli

Category: Products & Services > Competent Cells & Strains > Expression Competent Cells

One Shot® BL21 Star™ (DE3) E. coli are chemically competent cells designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems (e.g., Champion™ pET vectors) . One Shot® BL21 Star™ (DE3) Chemically Competent cells are controlled for a transformation efficiency of 1 x 10 8 cfu/ µg plasmid DNA. • A genotype that promotes high mRNA stability and protein yield • Ideal for use with low copy number, T7 promoter-based plasmids • The single tube, single use format allows all transformation steps to take place i...
Catalog: C6010-03
Size: 20 reactions