Essential Amino Acids Cell Culture
Gibco® MEM Amino Acids Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Amino Acids Solution is formulated to contain 50X the essential amino acids (except L-glutamine) found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Amino Acids Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufactur...
Catalog: 11130-051
Size: 100 ml
Size: 100 ml
Total Exosome Isolation (from cell culture media) enables fast and efficient enrichment of intact exosomes from cell culture media samples. • Maximize recovery of intact exosomes for any type of downstream application • Easily isolate exosomes using our simple and reliable protocol • Avoid time-consuming ultra-centrifugation • Maximize flexibility—can be scaled up or down depending on the sample size Isolation of exosomes can be a tedious, non-specific, and difficult process. Total Exosome Isolation (from cell culture media) enables simple and reliable concentration of intact exosomes from cel...
Catalog: 4478359
Size: 50 ml
Size: 50 ml
The CultureWell cell culture systems provide an integrated set-up for preparing cultured cells for staining and imaging. Each system uses medical-grade silicone gaskets preassembled with standard optical-quality coverslips in cell culture plates. The entire system is provided sterile and ready to use.
Catalog: C-24760
Size: 1 set
Size: 1 set
Exosome – Human CD63 Isolation/Detection enables the purification of CD63+ exosomes (also called extracellular vesicles and multi-vesicular bodies) from pre-enriched cell culture samples. These exosomes can then be detected using techniques such as flow cytometry, electron microscopy, or Western blotting. The exosomes must be pre-enriched before isolation. This can be done by ultracentrifugation, or by using the fast and efficient Total Exosome Isolation (from cell culture media) reagent. • Obtain highly pure CD63+ exosomes • "See" your sample during handling • Easily scalable protocol • Detec...
Catalog: 106-06D
Size: 3 ml
Size: 3 ml
The combination of the antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective action against gram-positive and gram-negative bacteria, respectively. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus . Streptomycin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibit...
Catalog: 15070-063
Size: 100 ml
Size: 100 ml
Fungizone® antimycotic is a formulation of Amphotericin B with sodium deoxycholate as an emulsifying agent to maintain the suspension. Amphotericin B is used to prevent the growth of fungi by causing an increase in fungal plasma membrane permeability. Gibco® Fungizone® antimycotic is used for the prevention of yeast and multicellular fungi contamination of cell cultures. Gibco® Fungizone contains 250 µg of amphotericin B and 205 µg of sodium deoxycholate per ml in distilled water. The recommended working concentration ranges from 0.25 to 2.50 µg/ml. Life Technologies offers a variety of antibi...
Catalog: 15290-018
Size: 20 ml
Size: 20 ml
StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. Accutase® does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex™, CELLStart™ and polymers. Accutase® performs exceptionally well in detaching primary and stem cells and maintaining high cell viabilty compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase® treatment include analysis of cell su...
Catalog: A11105-01
Size: 100 ml
Size: 100 ml
Essential 8™ Medium (published as E8) is a xeno-free and feeder-free medium specially formulated for the growth and expansion of human pluripotent stem cells (PSCs). Originally developed by Guokai Chen et al. (1) in the laboratory of James Thomson (published as "E8") and validated by Cellular Dynamics International, Essential 8™ Medium has been extensively tested and is proven to maintain pluripotency in multiple iPSC lines. In addition, Essential 8™ Medium has been used to scale up production of iPSCs and has been shown to support iPSC growth for >50 passages without any signs of karyotypic a...
Catalog: A14666SA
Size: 500 ml
Size: 500 ml
Essential 6™ Medium is a feeder-free and xeno-free medium that supports the reprogramming of somatic cells and differentiation of human pluripotent stem cells (PSCs). The formulation is based on a medium originally developed by Guokai Chen et al. (1) in the laboratory of James Thomson and published as "E8." Essential 6™ Medium has been proven to support somatic cell reprogramming using a variety of methods, including episomal vectors (2) and CytoTune™ (Sendai virus). In addition, Essential 6™ Medium can be used as a base for the formulation of custom media for the culture of PSCs. Essential 6™...
Catalog: A1516401
Size: 500 ml
Size: 500 ml
The cell-permeant SYTO 82 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.
Catalog: S11363
Size: 250 µl
Size: 250 µl
The cell-permeant SYTO 60 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.
Catalog: S11342
Size: 250 µl
Size: 250 µl
SYTOX® Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population. • Impermeant to live cells • Excitation/Emission: 504/523 nm • Use with 488 Argon-ion laser • Works with mammalian cells and both Gram-positive and Gram-negative bacteria Simple Viability Determination SYTOX® Green allows quick determination of cell viability when using flow cytometers, fluorescence microscopes, fluorometers, or fluorescence microplate readers. It will not cross intact membranes, ...
Catalog: S7020
Size: 250 µl
Size: 250 µl
Gibco® Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Phosphate Buffered Saline (PBS). Gibco® Cell Dissociation Buffer is suitable for gentle dissociation of mammalian cells for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry. Gibco® Cell Dissociation Buffer works well with lightly adherent cell lines such as HeLa and NIH 3T3, but is not recommended for routine passage of strongly adherent cells. The dis...
Catalog: 13151-014
Size: 100 ml
Size: 100 ml
DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence when bound to DNA. With NucBlue® Fixed Cell ReadyProbes™ Reagent, we have formulated a high-purity form of this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Features of NucBlue® Fixed Cell ReadyProbes™ Reagent: Ready-to-use liquid DAPI formulation No need to dilute, weigh, or pipette Convenient dropper bottle—just use two drops per ml Stable at room temperature—keep handy at your scope or...
Catalog: R37606
Size: 1 kit
Size: 1 kit
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • NEAA • L-glutamine • Phenol Red • HEPES
Catalog: 10370-088
Size: 10 × 500 ml
Size: 10 × 500 ml
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • HEPES • L-glutamine • Phenol Red
Catalog: 12360-038
Size: 500 ml
Size: 500 ml
MEM ALPHA
Catalog: 12714010S
Size: 500 ml
Size: 500 ml
Gibco® SensiCell™ MEM (Minimum Essential Medium) is an enriched basal medium that enhances growth of sensitive or difficult-to-culture mammalian cells with standard Fetal Bovine Serum (FBS) supplementation. Compared to classic MEM, SensiCell™ MEM is formulated with GlutaMAX™ and additional nutrients that can increase cell viability and viable cell density. SensiCell™ MEM requires supplementation, commonly with 10% Fetal Bovine Serum . Many cell lines do not require adaptation to this media. SensiCell™ MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 envi...
Catalog: A15156-01
Size: 500 ml
Size: 500 ml
Gibco® SensiCell™ MEM (Minimum Essential Medium) is an enriched basal medium that enhances growth of sensitive or difficult-to-culture mammalian cells with standard Fetal Bovine Serum (FBS) supplementation. Compared to classic MEM, SensiCell™ MEM is formulated with GlutaMAX™ and additional nutrients that can increase cell viability and viable cell density. SensiCell™ MEM requires supplementation, commonly with 10% Fetal Bovine Serum . Many cell lines do not require adaptation to this media. SensiCell™ MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 envi...
Catalog: A15156-02
Size: 10 × 500 ml
Size: 10 × 500 ml
Human Endothelial-SFM is a basal serum-free growth medium developed to support the isolation and expansion of human umbilical venous and arterial endothelial cells (1–4). Medium requires supplementation with bFGF (20 ng/ml), EGF (10 ng/ml), and human plasma fibronectin (10 µg/ml). Contains L-glutamine.
Catalog: 11111-044
Size: 500 ml
Size: 500 ml
Essential Amino Acids Cell Culture
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