Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Amino Acid Solutions
Gibco® MEM Amino Acids Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Amino Acids Solution is formulated to contain 50X the essential amino acids (except L-glutamine) found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Amino Acids Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufactur...
Catalog: 11130-051
Size: 100 ml
Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Reactive Oxygen & Nitrogen Species
DAF-FM is a reagent that is used to detect and quantify low concentrations of nitric oxide (NO). It is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. DAF-FM fluorescence can be detected by any instrument that can detect fluorescein, including flow cytometers, microscopes, fluorescent microplate readers and fluorometers. DAF-FM Specifications: • Ex/Em of DAF-FM: ~495/515 nm • Lyophilized product should be dissolved using DMSO and then added to an aqueous buffer to create a working solution • DAF-FM diacetate is cell permeant and passively diffuses across...
Catalog: D-23844
Size: 10 × 50 µg
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Cell, Tissue, & In Vivo Labeling Reagents
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Catalog: C2110
Size: 5 mg
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Stem Cell Media
Essential 8™ Medium (published as E8) is a xeno-free and feeder-free medium specially formulated for the growth and expansion of human pluripotent stem cells (PSCs). Originally developed by Guokai Chen et al. (1) in the laboratory of James Thomson (published as "E8") and validated by Cellular Dynamics International, Essential 8™ Medium has been extensively tested and is proven to maintain pluripotency in multiple iPSC lines. In addition, Essential 8™ Medium has been used to scale up production of iPSCs and has been shown to support iPSC growth for >50 passages without any signs of karyotypic a...
Catalog: A14666SA
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Stem Cell Media
Essential 6™ Medium is a feeder-free and xeno-free medium that supports the reprogramming of somatic cells and differentiation of human pluripotent stem cells (PSCs). The formulation is based on a medium originally developed by Guokai Chen et al. (1) in the laboratory of James Thomson and published as "E8." Essential 6™ Medium has been proven to support somatic cell reprogramming using a variety of methods, including episomal vectors (2) and CytoTune™ (Sendai virus). In addition, Essential 6™ Medium can be used as a base for the formulation of custom media for the culture of PSCs. Essential 6™...
Catalog: A1516401
Size: 500 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Anti-Phospho Amino Acid Antibodies
Clone/PAD: PY20 Immunogen: Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine Specificity: Reacts specifically with tyrosine phosphorylated proteins in the native or denatured states, and does not react with phosphoserine or phosphothreonine. Reacts with a wide variety of tyrosine-phosphorylated proteins in all tested species. Reactivity: Human, Mouse, (positive controls: mouse brain, NIH 3T3 cells (+/- TPA), K562 cells, EGF-stimulated A431 cells, and MCF-7 cell lysates) Applications: Western blotting, Immunoprecipitation, ELISA, Affinity chromotograp...
Catalog: 03-7722
Size: 100 µg
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Minimum Essential Media (MEM)
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • NEAA • L-glutamine • Phenol Red • HEPES
Catalog: 10370-088
Size: 10 × 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Minimum Essential Media (MEM)
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • HEPES • L-glutamine • Phenol Red
Catalog: 12360-038
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Minimum Essential Media (MEM)
MEM ALPHA
Catalog: 12714010S
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Minimum Essential Media (MEM)
Gibco® SensiCell™ MEM (Minimum Essential Medium) is an enriched basal medium that enhances growth of sensitive or difficult-to-culture mammalian cells with standard Fetal Bovine Serum (FBS) supplementation. Compared to classic MEM, SensiCell™ MEM is formulated with GlutaMAX™ and additional nutrients that can increase cell viability and viable cell density. SensiCell™ MEM requires supplementation, commonly with 10% Fetal Bovine Serum . Many cell lines do not require adaptation to this media. SensiCell™ MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 envi...
Catalog: A15156-01
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Minimum Essential Media (MEM)
Gibco® SensiCell™ MEM (Minimum Essential Medium) is an enriched basal medium that enhances growth of sensitive or difficult-to-culture mammalian cells with standard Fetal Bovine Serum (FBS) supplementation. Compared to classic MEM, SensiCell™ MEM is formulated with GlutaMAX™ and additional nutrients that can increase cell viability and viable cell density. SensiCell™ MEM requires supplementation, commonly with 10% Fetal Bovine Serum . Many cell lines do not require adaptation to this media. SensiCell™ MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 envi...
Catalog: A15156-02
Size: 10 × 500 ml