Enzyme Products
Gibco® Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Phosphate Buffered Saline (PBS). Gibco® Cell Dissociation Buffer is suitable for gentle dissociation of mammalian cells for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry. Gibco® Cell Dissociation Buffer works well with lightly adherent cell lines such as HeLa and NIH 3T3, but is not recommended for routine passage of strongly adherent cells. The dis...
Catalog: 13151-014
Size: 100 ml
Size: 100 ml
The RediPlate™ 96 EnzChek® Serine/Threonine Phosphatase Assay Kit provides a fast, simple and direct fluorescence-based assay for detecting serine/threonine phosphatases and their corresponding modulators and inhibitors. Unlike other microplate assays, this kit provides the necessary reagents predispensed into a 96-well microplate. Simply reconstitute the fluorogenic substrate in the assay wells with buffer, add the desired sample to the wells, incubate and then quantitate the fluorescence in any standard fluorescence-based microplate reader. Inhibitors are included in each assay well to ensur...
Catalog: R33700
Size: 1 kit
Size: 1 kit
Ambion® Ultrapure BSA is a "non-acetylated" BSA pure enough to use when DNA or RNA integrity is critical. One tube containing 50 mg is provided, at a concentration of 50 mg/mL. Bovine Serum Albumin (BSA) has many uses as a carrier protein and as a stabilizing agent in enzymatic reactions. In northern, Southern, and dot blot hybridizations, BSA is also used as a blocking agent. It is recommended in buffers for nick translation, polymerase reactions, and ligations. BSA is also a common additive for PCR amplifications, footprinting, and gel shift assays. In restriction digests, BSA has been shown...
Catalog: AM2616
Size: 1 tube
Size: 1 tube
PKN1 (PRK1) belongs to the protein kinase C superfamily. This kinase is activated by the Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can also be activated by phospholipids.
Catalog: PV3790
Size: 10 µg
Size: 10 µg
PI4KB and PI4KA are the two human type III PI4 kinases, which share sequence similarly with PI3 kinases and phosphorylate the 4 position of phosphotidyl inositol.
Catalog: PV5277
Size: 20 µg
Size: 20 µg
BMPR1A is a Type I member of the TGF beta receptor superfamily of transmembrane ser⁄thr kinases which phosphorylates intracellular SMADs in response to bone morphogenic proteins.
Catalog: PV6038
Size: 10 µg
Size: 10 µg
Recombinant Mouse Fibroblast Growth Factor Basic (also known as FGF Basic, bFGF, FGF 2, FGF-β or Heparin Binding Growth Factor) is a bioactive protein intended for use in cell culture applications. FGF-basic is involved in a number of biological processes including embryonic development and differentiation, neuron differentiation, survival, and regeneration, and the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. • High purity —no interference from other proteins or contaminants • High biological activity —more results with less...
Catalog: PMG0031
Size: 100 µg
Size: 100 µg
MAP3K14 (NIK) is structurally related to MAP3Ks. MAP3K14 phosphorylates and activates CHUK (IKKalpha) and IKBKB (IKK beta), which in turn phosphorylate IkB-alpha, releasing NF-KappaB leading to up-regulation of inflammatory response genes.
Catalog: PV4902
Size: 10 µg
Size: 10 µg
KDR (VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors.
Catalog: PV3660
Size: 10 µg
Size: 10 µg
EIF2AK3 (PERK) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK3, signaling is initiated by misfolded proteins in the ER.
Catalog: PV5106
Size: 10 µg
Size: 10 µg
Retinoid-related orphan receptor gamma, ligand binding domain (RORG-LBD) is a 58.60 kDa recombinant human protein (amino acids 250-518) expressed as a GST-tagged fusion protein in insect cells. RORG-LBD was expressed and purified in the absence of exogenous ligands, making it ideal for ligand binding and coregulator displacement studies.
Catalog: PV5888
Size: 50 μg
Size: 50 μg
CDK8 phosphorylates the carboxy-terminal domain of the largest subunit of RNA polymerase II. This protein is coexpressed and copurified with CyclinC.
Catalog: PV4402
Size: 10 µg
Size: 10 µg
Ambion® Acetylated BSA is used as a carrier protein and as an enzyme stabilizer in which the absence of contaminants such as nucleases and proteases is essential. Six tubes of 25 mg are provided, at a concentration of 40–50 mg/mL. The acetylation process inactivates any trace nuclease activity that might be present. While the acetylation process does change BSA's binding characteristics and makes it inhibitory in PCR reactions, it is routinely added to restriction digests. The molecular weight is ~66 kD. Acetylated BSA is rigorously tested for contaminating nonspecific endonuclease, exonucleas...
Catalog: AM2614
Size: 6 tubes
Size: 6 tubes
MAPK9 (JNK2) is a serine⁄threonine kinase which plays an important role in the MAPK pathway.
Catalog: PV4018
Size: 1 mg
Size: 1 mg
Cluster of Differentiation 44 (CD44), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Pro 220) of human CD44 (P16070-1) fused to a polyhistidine tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Trehalose, and 0...
Catalog: 12211-H08H-5
Size: 5 µg
Size: 5 µg
HIV-1 glycoprotein 120 (gp120, fragment of env), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the HIV-1 surface glycoprotein gp120 (Ala 29-Glu 503), which is cleaved from the full length envelope glycoprotein gp160, fused to a polyhistidine tag at the C-terminus and a si...
Catalog: 11233-V08H-250
Size: 5 × 50 µg
Size: 5 × 50 µg
HIV-1 glycoprotein 120 (gp120, fragment of env), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the HIV-1 surface glycoprotein gp120 (Ala 29-Glu 503), which is cleaved from the full length envelope glycoprotein gp160, fused to a polyhistidine tag at the C-terminus and a si...
Catalog: 11233-V08H-5
Size: 5 µg
Size: 5 µg
HIV-1 glycoprotein 120 (gp120, fragment of env), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the HIV-1 surface glycoprotein gp120 (Ala 29-Glu 503), which is cleaved from the full length envelope glycoprotein gp160, fused to a polyhistidine tag at the C-terminus and a si...
Catalog: 11233-V08H-50
Size: 50 µg
Size: 50 µg
Ephrin-B1 (EFNB1), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 232) of human EFNB1 (NP_004420.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized mouse EFNB3 at 2 µg/ml (100 µl/well) can bind human EFNB1-Fc wit...
Catalog: 10894-H03H-25
Size: 25 µg
Size: 25 µg
Ephrin-B1 (EFNB1), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 232) of human EFNB1 (NP_004420.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized mouse EFNB3 at 2 µg/ml (100 µl/well) can bind human EFNB1-Fc wit...
Catalog: 10894-H03H-250
Size: 5 × 50 µg
Size: 5 × 50 µg
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