Endothelial Cell Supplements
Human Endothelial-SFM is a basal serum-free growth medium developed to support the isolation and expansion of human umbilical venous and arterial endothelial cells (1–4). Medium requires supplementation with bFGF (20 ng/ml), EGF (10 ng/ml), and human plasma fibronectin (10 µg/ml). Contains L-glutamine.
Catalog: 11111-044
Size: 500 ml
Size: 500 ml
N-2 Supplement is a chemically defined, serum-free supplement based on Bottenstein’s N-1 formulation. N-2 Supplement is intended for use with Neurobasal® media. N-2 Supplement is recommended for growth and expression of neuroblastomas as well as post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS). N-2 Supplement can be used as a substitute for Bottenstein’s N-1 formulation. N-2 Supplement is provided as a 100X concentrate. N-2 Supplement may be used with Neurobasal® media supplemented with growth factors such as bFGF and E...
Catalog: 17502-048
Size: 5 ml
Size: 5 ml
Gibco® HAT Supplement is a liquid mixture of sodium hypoxanthine (5 mM), aminopterin (20 µM) and thymidine (0.8 mM). HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT–myeloma cells. Hypoxanthine and thymidine supply preformed purines and pyrimidines for DNA synthesis by hybridomas via the salvage pathway that utilizes HGPRT- contributed by the fused spleen cell. Aminopterin, a folic acid antagonist, inhibits the de novo nucleoside biosynthesis pathway. Product Use For Research Use Only. Not for use in diagnostic procedures. Dual site cGMP Manufac...
Catalog: 21060-017
Size: 100 ml
Size: 100 ml
A sterile set of solutions intended for use with EpiLife® medium or Medium 154 to culture human epidermal keratinocytes. The HKGS Kit provides, in separate vials, all the components of complete HKGS (S-001-5). A vial of gentamycin/amphotericin solution (GA) is also included. Use of GA is optional.
Catalog: S-001-K
Size: 1 kit
Size: 1 kit
A defined, sterile, concentrated (100X) solution intended for use with EpiLife® medium to culture human epidermal keratinocytes. EDGS is not intended for use with Medium 154. EDGS contains all of the growth factors and hormones necessary for the growth of human epidermal keratinocytes. Each bottle contains the correct amount of supplement for a 500 ml bottle of EpiLife® medium. EDGS contains: • purified bovine serum albumin • purified bovine transferrin • hydrocortisone • recombinant human insulin-like growth factor type-1 (rhIGF-1) • prostaglandin E2 (PGE2) • recombinant human epidermal growt...
Catalog: S-012-5
Size: 5 ml
Size: 5 ml
Knockout™ Serum Replacement (Knockout™ SR) is a defined, serum-free formulation used to support the growth of undifferentiated ES (Embryonic Stem Cells) and iPS (Induced Pluripotent Stem Cells) in culture. It directly replaces FBS in existing protocols. One of the most important parameters surrounding ES cell culture is the maintenance of ES cells in an undifferentiated state. Until now, FBS was the only option for nutritive supplementation of ES and iPS cell media. Unfortunately, FBS also contains factors which can promote differentiation of ES cells. Thus, each lot must be screened prior to ...
Catalog: 10828010
Size: 100 ml
Size: 100 ml
The protein concentration of each lot of Bovine Pituitary Extract is determined by the Bradford method. Each vial of BPE contains not less than 25 mg of protein.
Catalog: 13028-014
Size: 25 mg
Size: 25 mg
7.5 g/100 ml Prepared with Bovine Serum Albumin Fraction V in phosphate-buffered saline.
Catalog: 15260-037
Size: 100 ml
Size: 100 ml
Gibco® Insulin, Transferrin, Selenium, Sodium Pyruvate Solution (ITS -A) is used as a basal medium supplement in order to reduce the amount of Fetal Bovine Serum (FBS) used to culture the cells. Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G is now routinely used with other basal media to support a wide variety of cell types in the presence of 2-4% FBS. Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and it may also help to reduce toxic levels of oxyge...
Catalog: 51300-044
Size: 10 ml
Size: 10 ml
Gibco® MEM Vitamin Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Vitamin Solution is formulated to contain 100x the vitamins found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Vitamin Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001...
Catalog: 11120-052
Size: 100 ml
Size: 100 ml
Gibco® MEM Amino Acids Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Amino Acids Solution is formulated to contain 50X the essential amino acids (except L-glutamine) found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Amino Acids Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufactur...
Catalog: 11130-051
Size: 100 ml
Size: 100 ml
Gibco® 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. Mercaptoethanol is not stable in solution so most protocols require daily supplementation. Gibco® 2-Mercaptoethanol is made at 50 mM in Dulbecco's Phosphate Buffered Saline (DPBS). Product Use For Research Use Only. Not for use in diagnostic procedures. cGMP Manufacturing and Quality System Gibco® 2-Mercaptoethanol is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the...
Catalog: 31350-010
Size: 20 ml
Size: 20 ml
Sodium pyruvate is commonly added to cell culture media as an energy source in addition to glucose. Since cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not a required supplement for all cell cultures. However, if cells have been grown in medium that is supplemented with sodium pyruvate, we recommend continuing use of the supplement as cell growth may lag without it. Gibco® Sodium Pyruvate (100 mM) is formulated using 11 g per liter of water. The final concentration of sodium pyruvate used in most cell culture media is 1 mM. Dual-site cGMP Manufacturi...
Catalog: 11360-070
Size: 100 ml
Size: 100 ml
Endothelial Cell Supplements
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