Embryonic Cells

Gibco® Mouse (C57BL⁄6) Embryonic Stem Cells

Category: Products & Services > Eukaryotic Cells > Stem Cells > Embryonic Stem Cells

Mouse Embryonic stem cells (ESCs) were isolated from mouse (Strain C57BL⁄6) embryos at Day E3.5. Before cryopreservation, the cells were tested for viability (>70% post-thaw viability), their ability to remain undifferentiated when cultured on mouse feeder cells as assayed by marker expression (Oct4, SSEA-1, Nanog) and their ability to differentiate into embryoid bodies and express markers indicative of three germ layers (Ectoderm: (+) for β3-tubulin and nestin, Mesoderm: (+) for smooth muscle actin, Endoderm: (+) for AFP). Cells were cryopreserved at passage 20 (P20). For optimal growth perfo...

Catalog: S1503-100
Size: 1 ml

Gibco® PSC Neural Induction Medium (prototype)

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Stem Cell Media

Gibco® PSC Neural Induction Medium is a serum-free medium that provides high efficiency neural induction of human pluripotent stems cells (PSCs) in only 7 days. Unlike existing methodologies, use of Gibco® PSC Neural Induction Medium does not require the intermediary step of embryoid body (EB) formation, which adds time, labor, and variability. Neural Stem Cells (NSCs) generated using Gibco® PSC Neural Induction Medium have high expression of NSC markers and can be further differentiated into other neural cell types. Note: The "prototype" status of this product means that the product is curren...

Catalog: A15640SA
Size: 500 ml

KnockOut™ Serum Replacement

Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Serum Replacements

Knockout™ Serum Replacement (Knockout™ SR) is a defined, serum-free formulation used to support the growth of undifferentiated ES (Embryonic Stem Cells) and iPS (Induced Pluripotent Stem Cells) in culture. It directly replaces FBS in existing protocols. One of the most important parameters surrounding ES cell culture is the maintenance of ES cells in an undifferentiated state. Until now, FBS was the only option for nutritive supplementation of ES and iPS cell media. Unfortunately, FBS also contains factors which can promote differentiation of ES cells. Thus, each lot must be screened prior to ...

Catalog: 10828010
Size: 100 ml

Expi293™ Expression Medium

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > 293 Media

Expi293™ Expression Medium is a chemically-defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density cultures of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression. • Supports growth of suspension 293 cultures to densities of over 1.6 x 10 7 cells/mL, with >95% cell viability • Transfection-compatible serum-free suspension culture medium allowing transfection efficiencies of greater than 70% using ExpiFectamine...

Catalog: A1435101
Size: 1000 ml