Electrophoretic Mobility Shift

Protein Thermal Shift™ Starter Kit

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Protein Analysis Kits

Applied Biosystems® Protein Thermal Shift™ kits allow users to employ a dye that binds to exposed hydrophobic residues to monitor the thermal stability of proteins using a real-time PCR instrument in order to identify buffer conditions that stabilize the protein of interest or screen libraries of ligands for compounds that bind the protein of interest. Save Time and Money with Your Sample Screening This solution significantly reduces the time and cost associated with screening samples relative to currently available methods. Protein Thermal Shift™ products that are run on our real-time PCR sys...

Catalog: 4462263
Size: 2000 reactions

Protein Thermal Shift™ Software v1.0--Additional License

Category: Products & Services > Instruments, Software, & Accessories > Software > Real-Time PCR Software

The Protein Thermal Shift™ Software v1.0 was developed to analyze protein melt fluorescent readings directly from Applied Biosystems® real-time PCR instrument files. Different proteins will have different Protein Thermal Shift™ profiles, each with a unique protein melt curve shape, slope, signal-to-noise ratio, and temperature melt range (see figure at right for an example). The Protein Thermal Shift™ Software generates one or multiple melting temperature values (Tm) from these curves by two methods: the Boltzmann-derived Tm and the Derivative Curve-determined Tm, to serve as points of compari...

Catalog: 4466038
Size: 1 license

BRAF Mutation Analysis Reagents

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Genetic & Chromosome Abnormality Detection Kits

The BRAF Mutation Analysis Reagents employ a simple fragment analysis workflow to detect 1% mutant to wild type genomic DNA across 3 common and rare mutations in the BRAF gene region (see Table 1). The BRAF set provides sufficient reagent for analysis of 30 samples. Advantages of the BRAF Mutation Analysis Reagents include: • Simple Analysis—unambiguous, easy-to-interpret results • Thorough Coverage—detects 3 mutations in the BRAF gene • Sensitive—detects mutations at frequencies as low as 1 to 5% against a wild type genomic DNA background • Efficient—interrogate multiple loci from the same sa...

Catalog: 4452136
Size: 1 kit