Electrophoresis Buffers
UltraPure™ Agarose is a polysaccharide (see structure below) used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Agarose is ideal for resolving DNA and RNA fragments from 500 bp to > 20 kb. • Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handling and less breakage • Can be used in protein electrophoresis applications such as Ouchterlony (antigen-antibody interaction assay) and radial immunodiffusion (RID) (antigen quantitation assay) Improved Packaging The new environmentally ...
Catalog: 16500100
Size: 100 g
Size: 100 g
UltraPure™ Low Melting Point Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Low Melting Point Agarose is ideal for resolving DNA and RNA fragments larger than 1,000 bp and for the recovery of nucleic acid fragments after electrophoresis, as it melts at 65.5°C (below the melting point of most nucleic acids). The solution remains fluid at 37°C and will set rapidly at temperatures below 25°C. • Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handlin...
Catalog: 16520100
Size: 100 g
Size: 100 g
Replacement Parts for Electrophoresis Apparatus
Catalog: EI0012
Size: each
Size: each
NuPAGE® LDS Sample Buffer (4X) (10 ml) is used to prepare protein samples for denaturing gel electrophoresis with the NuPAGE® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing the protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate at a pH of 8.4, which allows for maximal activity of the reducing agent. For native gel electrophoresis, use Novex® Tris-Glycine Native Sample Buffer (2X) . Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sample...
Catalog: NP0007
Size: 10 ml
Size: 10 ml
NuPAGE® LDS Sample Buffer (4X) (250 ml) is used to prepare protein samples for denaturing gel electrophoresis with the NuPAGE® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing the protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate at a pH of 8.4, which allows for maximal activity of the reducing agent. For native gel electrophoresis, use Novex® Tris-Glycine Native Sample Buffer (2X) . Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sampl...
Catalog: NP0008
Size: 250 ml
Size: 250 ml
Bolt® Transfer Buffer is optimized for the transfer of proteins from Bolt® Bis-Tris Plus gels to membranes for western blotting. When combined with 10% methanol, Bolt® Transfer Buffer can be used with the Bolt® Mini Blot Module for wet transfer. Bolt® Transfer Buffer can also be used with Bolt® Antioxidant to enhance transfer of reduced proteins to membranes. About the Bolt® Mini Blot Module The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout....
Catalog: BT0006
Size: 125 ml
Size: 125 ml
Bolt® Transfer Buffer is optimized for the transfer of proteins from Bolt® Bis-Tris Plus gels to membranes for western blotting. When combined with 10% methanol, Bolt® Transfer Buffer can be used with the Bolt® Mini Blot Module for wet transfer. Bolt® Transfer Buffer can also be used with Bolt® Antioxidant to enhance transfer of reduced proteins to membranes. About the Bolt® Mini Blot Module The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout....
Catalog: BT00061
Size: 1 L
Size: 1 L
Denhardt's Solution is a mixture of blocking agents used in membrane-based hybridization protocols. The solution contains 1% Ficoll (type 400), 1% polyvinylpyrrolidone, and 1% bovine serum albumin. Sterile filtered.
Catalog: 750018
Size: 100 ml
Size: 100 ml
Ambion® Acrylamide/Bis 19:1 is a 40% (w/v) solution of acrylamide (38%) and bis-acrylamide (2%) ideal for use in ribonuclease protection assay, sequencing gels, and sizing DNA or RNA fragments. Supplied in one bottle containing 500 mL. The solution is provided in a ready-to-use form, reducing the dust, inhalation, and contact hazard associated with weighing and preparing acrylamide and bis-acrylamide powders and solutions. Concentrations are carefully controlled and all solutions are micro-filtered prior to packaging. All Ambion® acrylamides are rigorously tested for contaminating non-specific...
Catalog: AM9022
Size: 500 ml
Size: 500 ml
NuPAGE® Antioxidant is a proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis. NuPAGE® Antioxidant is added to the running buffer in the upper (cathode) buffer chamber when performing protein gel electrophoresis under reducing conditions. NuPAGE® Antioxidant is provided in a 15 ml size. NuPAGE® Antioxidant must be used with samples reduced using NuPAGE® Sample Reducing Agent or other reducing agent. NuPAGE® Antioxidant is optimized for the neutral pH of NuPAGE® gels and is not compatible with other gel chemistries. NuPAGE® Antioxidant may also be ad...
Catalog: NP0005
Size: 15 ml
Size: 15 ml
Molecular biology grade, Ambion® 10X TBE solution is supplied in four bottles containing 1 L each. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating...
Catalog: AM9865
Size: 4 bottle
Size: 4 bottle
Gibco® 4% Agarose is an autoclaved, high purity, low melting point gel designed for plaque assays and the purification of baculovirus. The 4% Agarose Gel melts at 65 – 70°C and remains fluid at 37°C. When diluted with concentrated insect cell culture medium, Gibco® 4% Agarose produces a gel firm enough to immobilize local infections, yet ductile enough to allow for the efficient removal of plaques for clonal expansion. Product Use For Research Use Only. Not for use in diagnostic procedures.
Catalog: 18300-012
Size: 40 ml
Size: 40 ml
NativePAGE™ 5% G-250 Sample Additive is added to samples prepared with the NativePAGE™ Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE™ gels. This Additive is also included in the NativePAGE™ Sample Prep Kit. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis showing increased resolution and reduced streaking.
Catalog: BN2004
Size: 0.5 ml
Size: 0.5 ml
Bolt® Antioxidant is a proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis. Bolt® Antioxidant migrates with reduced proteins to maintain reducing conditions and to prevent re-oxidation of sensitive amino acids such as methionine and tryptophan. Bolt® Antioxidant must be used with samples reduced using Bolt® Sample Reducing Agent or other reducing agent. Bolt® Antioxidant is optimized for the neutral pH of Bolt® Bis-Tris Plus gels and is not compatible with other gel chemistries. Bolt® Antioxidant can also be used with Bolt® Transfer Buffer to enhan...
Catalog: BT0005
Size: 15 ml
Size: 15 ml
Electrophoresis Buffers
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