Electrocompetent E Coli Pir1 Cells
The Echo™ Cloning System is a two-vector system that utilizes the Cre-lox site-specific recombination system of bacteriophage P1 (1,2) for rapid cloning into multiple vectors. Your gene of interest is cloned into one of the specially designed donor vectors which can then be recombined with as many different Echo™ acceptor vectors as desired. The donor and acceptor vectors are placed in a buffer that contains Cre recombinase for a 25-minute, in vitro recombination reaction. Cre binds to the lox site present on each vector, brings them together, then cleaves and covalently reattaches the DNA str...
Catalog: C1010-10
Size: 10 reactions
Size: 10 reactions
Trypan blue stain is a well-accepted method for staining dead cells. It can enter into cells with compromised membranes and is the method used with the Countess® automated cell counting platform.
Catalog: T10282
Size: 2 × 1 ml
Size: 2 × 1 ml
StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. Accutase® does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex™, CELLStart™ and polymers. Accutase® performs exceptionally well in detaching primary and stem cells and maintaining high cell viabilty compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase® treatment include analysis of cell su...
Catalog: A11105-01
Size: 100 ml
Size: 100 ml
ElectroMAX™ DH10B™ T1 Phage-Resistant electrocompetent E. coli cells offer transformation efficiencies that are among the highest achieved by any product offered by Life Technologies, and can exceed 1 x 10 10 cfu/ µg plasmid DNA in efficiency. They are ideal for applications requiring high efficiency transformation, including cDNA and gDNA library construction. • Maximizes yield of transformants from limited cloning products • Permits efficient cloning of methylated DNA • Resistant to T1 and T5 bacteriophages • Supports blue/white screening by α-complementation on plates containing X-Gal or Bl...
Catalog: 12033-015
Size: 0.5 ml
Size: 0.5 ml
StemPro® CD34+ cells are pooled Human Hematopoietic Progenitor cells (HPCs) derived from the umbilical cord blood of mixed donors. Along with 0.5×10 6 pooled donor cord blood-derived CD34+ cells, this kit includes StemPro®-34 SFM basal liquid medium and frozen StemPro®-34 Nutrient Supplement to facilitate the immediate culturing of the StemPro® CD34+ cells upon arrival or when recovering from cryopreservation storage. With the StemPro® CD34+ Cell Kit You Get: • ≥90% purity of the desired CD34+ cell population • Each vial of cells contains 0.5×10 6 CD34+ cells with full traceability • StemPro®-...
Catalog: A14059
Size: 0.5 x 10
Size: 0.5 x 10
Mouse Embryonic stem cells (ESCs) were isolated from mouse (Strain C57BL⁄6) embryos at Day E3.5. Before cryopreservation, the cells were tested for viability (>70% post-thaw viability), their ability to remain undifferentiated when cultured on mouse feeder cells as assayed by marker expression (Oct4, SSEA-1, Nanog) and their ability to differentiate into embryoid bodies and express markers indicative of three germ layers (Ectoderm: (+) for β3-tubulin and nestin, Mesoderm: (+) for smooth muscle actin, Endoderm: (+) for AFP). Cells were cryopreserved at passage 20 (P20). For optimal growth perfo...
Catalog: S1503-100
Size: 1 ml
Size: 1 ml
The Violet Ratiometric Membrane Asymmetry Probe⁄Dead Cell Apoptosis Kit provides a simple and fast method for the detection of apoptosis with dead cell discrimination by flow cytometry. The Violet Ratiometric Membrane Asymmetry Probe, 4'-N,N-diethylamino-6-(N,N,N-dodecyl-methylamino-sulfopropyl)-methyl-3-hydroxyflavone (F2N12S), is a novel violet excitable dye for the detection of membrane asymmetry changes during apoptosis. The dye exhibits an excited-state intramolecular proton transfer (ESIPT) reaction resulting in a dual fluorescence with two emission bands corresponding to 530 nm and 585 ...
Catalog: A-35137
Size: 1 kit
Size: 1 kit
MAX Efficiency® Stbl2™ Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts. In addition to rec A1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences (1) and tandem array genes (2). MAX Efficiency® Stbl2™ Competent Cells offer: >1 x 10 9 transformants/flg for cloning rare sequences Elimination of mcr BC- hsd RMS- mrr and mutation of mcr A allows cloning of methylated genomic sequences (1) Unique genotype stabilizes direct repeat and retroviral sequences such as SIV and HIV (1) Greatly ...
Catalog: 10268-019
Size: 1 ml
Size: 1 ml
For Academic pricing please login or contact us at discoverysciences@invitrogen.com. The Tango™ CCR5- bla U2OS cells contain the human Chemokine (C-C Motif) Receptor 5 (CCR5) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR- bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase ( bla ) reporter gene under the control of a UAS response element. The Tango™ CCR5- bla U2OS cells are functionally validated for Z' and EC 50 concentrations of MIP-1a. In addition,...
Catalog: K1788
Size: 8 × 10^6 cells
Size: 8 × 10^6 cells
BL21(DE3)pLysE E. coli are ideal for use with T7 promoter-based expression vectors. Like BL21(DE3)pLysS E. coli , BL21(DE3)pLysE carry the lambda DE3 lysogen. In addition, BL21(DE3)pLysE cells contain the pLysE plasmid, which constitutively expresses T7 lysozyme. T7 lysozyme reduces the basal expression of target genes by inhibiting T7 RNA polymerase. The pLysE plasmid in BL21(DE3)pLysE expresses higher levels of T7 lysozyme than the pLysS plasmid in BL21(DE3)pLysS. The BL21(DE3)pLysE strain provides tighter control of T7 RNA polymerase, which is necessary when the recombinant protein to be ex...
Catalog: C6565-03
Size: 20 × 50 µl
Size: 20 × 50 µl
The ER-Tracker™ Red dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained using the protocol provided, the staining pattern is partially retained after fixation with formaldehyde. This stain consists of the green-fluorescent BODIPY® TR dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K + channels which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell typ...
Catalog: E34250
Size: 100 µg
Size: 100 µg
MAX Efficiency® DH10Bac™ Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac® Baculovirus Expression System . The DH10Bac™ E. coli strain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac™, to create an expression bacmid containing a cloned gene of interest. Features of MAX Efficiency® DH10Bac™ Competent Cells include: • Consistent yields of >1 × 10 8 transformants/ µg • Tolerance of small amounts of undiluted ligation reactions • Support of efficient blue/white colony screening (Φ80 lac ZΔM15 marker) About MAX Ef...
Catalog: 10361-012
Size: 5 × 100 µl
Size: 5 × 100 µl
Electrocompetent E Coli Pir1 Cells
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