Dynabeads Insulin, Transferrin & Iron Supplements
Gibco® Insulin, Transferrin, Selenium, Sodium Pyruvate Solution (ITS -A) is used as a basal medium supplement in order to reduce the amount of Fetal Bovine Serum (FBS) used to culture the cells. Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G is now routinely used with other basal media to support a wide variety of cell types in the presence of 2-4% FBS. Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and it may also help to reduce toxic levels of oxyge...
Catalog: 51300-044
Size: 10 ml
Size: 10 ml
Dynabeads® Sheep-anti Rat IgG binds monoclonal rat IgGs of your choice for specific cell isolation or depletion. All cells from all species except from rat can be isolated with this product, depending only on the specific antibody of choice. Especially well suited for cell isolation from mouse samples. Depletion: cells can be depleted from a sample Positive isolation: cells can be positively isolated from a sample for molecular applications Negative isolation: cells can be negatively isolated from a sample (i.e. spleen or lymph node cells) by adding an antibody cocktail of rat IgGs towards the...
Catalog: 11035
Size: 5 ml
Size: 5 ml
An excellent tool for highly predictable and consistent extraction and isolation of nucleic acids from biological samples, following a simple magnetic separation protocol. Please note that this is a stand-alone product containing only Dynabeads® in a storage buffer. Our products Dynabeads® SILANE genomic DNA (Cat.no. 370-12D) or Dynabeads® SILANE viral NA (Cat.no. 370-11D) include the appropriate buffers and protocols you need to extract genomic DNA or viral nucleic acids respectively. Magnetic particles from alternative suppliers often have a random size range distribution, surface area and b...
Catalog: 37002D
Size: 5 ml
Size: 5 ml
Gibco® HAT Supplement is a liquid mixture of sodium hypoxanthine (5 mM), aminopterin (20 µM) and thymidine (0.8 mM). HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT–myeloma cells. Hypoxanthine and thymidine supply preformed purines and pyrimidines for DNA synthesis by hybridomas via the salvage pathway that utilizes HGPRT- contributed by the fused spleen cell. Aminopterin, a folic acid antagonist, inhibits the de novo nucleoside biosynthesis pathway. Product Use For Research Use Only. Not for use in diagnostic procedures. Dual site cGMP Manufac...
Catalog: 21060-017
Size: 100 ml
Size: 100 ml
Dynabeads® CD4 are magnetic beads that provide a robust and versatile tool to isolate or deplete human CD4 + T-cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. • Rapid isolation of CD4 + T-cells from any sample – no columns required • Positive isolation for molecular downstream assays or efficient depletion of CD4 + T cells Excellent Yield, Purity, and Viability of Recovered Cells Dynabeads® CD4 are uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of human CD4 + cells directly from any samp...
Catalog: 11145D
Size: 5 ml
Size: 5 ml
A sterile set of solutions intended for use with EpiLife® medium or Medium 154 to culture human epidermal keratinocytes. The HKGS Kit provides, in separate vials, all the components of complete HKGS (S-001-5). A vial of gentamycin/amphotericin solution (GA) is also included. Use of GA is optional.
Catalog: S-001-K
Size: 1 kit
Size: 1 kit
N-2 Supplement is a chemically defined, serum-free supplement based on Bottenstein’s N-1 formulation. N-2 Supplement is intended for use with Neurobasal® media. N-2 Supplement is recommended for growth and expression of neuroblastomas as well as post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS). N-2 Supplement can be used as a substitute for Bottenstein’s N-1 formulation. N-2 Supplement is provided as a 100X concentrate. N-2 Supplement may be used with Neurobasal® media supplemented with growth factors such as bFGF and E...
Catalog: 17502-048
Size: 5 ml
Size: 5 ml
Streptavidin-coupled Dynabeads® is the gold standard for isolation and handling of biotinylated nucleic acids, antibodies or other biotinylated ligands and targets. The very high binding affinity of the streptavidin-biotin interaction (Kd=10 -15 ) is utilized in a vast number of applications. These uniform and superparamagnetic beads are 1 µm in diameter, with a monolayer - not a multilayer - of recombinant streptavidin covalently coupled to the surface and further blocked with BSA. The monolayer of streptavidin leaves the vast majority of the biotin binding sites sterically available for bind...
Catalog: 65602
Size: 10 ml
Size: 10 ml
Enrich epithelial tumor cells directly from whole blood, bone marrow, or PBMC suspensions. Enriched tumor cells are lysed for mRNA isolation and RT-PCR amplification. The sensitivity of combined enrichment and RT-PCR detection can be as high as 1 tumor cell in 1 ml blood. Depletion of epithelial tumor cells from whole blood/bone marrow or PBMC. Positive isolation of epithelial tumor cells for downstream molecular analysis. No columns required Starting samples: Whole blood, bone marrow, PBMC, buffy coat. Number of cells processed: 200 ml whole blood or ~4 × 10 9 PBMC.
Catalog: 16102
Size: 5 ml
Size: 5 ml
A defined, sterile, concentrated (100X) solution intended for use with EpiLife® medium to culture human epidermal keratinocytes. EDGS is not intended for use with Medium 154. EDGS contains all of the growth factors and hormones necessary for the growth of human epidermal keratinocytes. Each bottle contains the correct amount of supplement for a 500 ml bottle of EpiLife® medium. EDGS contains: • purified bovine serum albumin • purified bovine transferrin • hydrocortisone • recombinant human insulin-like growth factor type-1 (rhIGF-1) • prostaglandin E2 (PGE2) • recombinant human epidermal growt...
Catalog: S-012-5
Size: 5 ml
Size: 5 ml
Use this kit to isolate a high purity and yield of human CD34+ progenitor stem cells. Positively isolated cells are bead and antibody-free, phenotypically unaltered and suitable for any downstream applications including flow cytometry, functional studies, and cell culture to produce dendritic cells (DC's). The Dynal® CD34 Progenitor Cell Isolation System can also be used to isolate CD34+ cells from rhesus monkey (Macaca mulatta). Positive isolation of human CD34+ progenitor stem cells with bead release Stem cells can be isolated directly from whole blood, cord blood or bone marrow Isolated CD3...
Catalog: 11301D
Size: 5 ml
Size: 5 ml
Our Magnetic Particle Concentrators (MPC®) enable optimal binding of Dynabeads® magnetic beads with their targets (e.g., cells, proteins, and nucleic acids) in a variety of starting samples. The MPC®-S was designed specifically for microbiology applications; it has been validated with select Dynabeads® for enrichment of E. coli O157:H7, Salmonella, non-O157 STEC, Cryptosporidium, and Giardia. The MPC®-S can be used with both food enrichment samples types as well as water samples. It holds up to six microcentrifuge tubes and has an optimal working volume range of 20 µl–2 ml. Note that this magn...
Catalog: A13346
Size: 1 unit
Size: 1 unit
RHU INSULIN
Catalog: 0045077II
Size: 1 each
Size: 1 each
The Ion AmpliSeq™ Dynabeads® Cleanup Module is a magnetic bead-based purification kit used in the construction of RNA libraries for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ sequencer. This module is included in the Ion AmpliSeq™ RNA Library Kit, but may be purchased separately if additional modules are needed. For RNA library construction using the Ion AmpliSeq™ RNA Library Kit, the module is used for cleanup after the cDNA synthesis and PCR amplification steps. The module employs Dynabeads® magnetic beads for DNA adsorption and separation, rather than traditional f...
Catalog: 4482437
Size: 96 reactions
Size: 96 reactions
This is a purified rabbit anti-human Insulin Receptor Polyclonal Antibody, that can be used in the validated applications of Western blot, ELISA, and immunohistochemistry. This Insulin Receptor Polyclonal Antibody was produced in rabbits immunized with purified, recombinant human Insulin Receptor. Insulin Receptor specific IgG was purified by human Insulin Receptor affinity chromatograpy. Antibody characteristics include: Applications: This Insulin Receptor Polyclonal Antibody, has been validated for use in Western blot, ELISA, and immunohistochemistry Host species and isotype: This is a rabbi...
Catalog: 11081-RP02-AB
Size: 100 µg
Size: 100 µg
This is a purified rabbit anti-human Transferrin Receptor Polyclonal Antibody, that can be used in the validated applications of Western blot, ELISA, and immunohistochemistry. This Transferrin Receptor Polyclonal Antibody was produced in rabbits immunized with purified, recombinant human Transferrin Receptor. Transferrin Receptor specific IgG was purified by human Transferrin Receptor affinity chromatograpy. Antibody characteristics include: Applications: This Transferrin Receptor Polyclonal Antibody, has been validated for use in Western blot, ELISA, and immunohistochemistry Host species and ...
Catalog: 11020-RP02-AB
Size: 100 µg
Size: 100 µg
This is one of a group of CaptureSelect™ protein purification affinity resins developed for the simple, single-step purification of a variety human plasma and serum proteins. This unique selection of affinity purification resins can purify proteins or other non-antibody biomolecules from either recombinant or native sources with a high degree of affinity and specificity, and are immobilized on a high capacity agarose support.
Catalog: 191306005
Size: 5 ml
Size: 5 ml
This is one of a group of CaptureSelect™ protein purification affinity resins developed for the simple, single-step purification of a variety human plasma and serum proteins. This unique selection of affinity purification resins can purify proteins or other non-antibody biomolecules from either recombinant or native sources with a high degree of affinity and specificity, and are immobilized on a high capacity agarose support.
Catalog: 191306010
Size: 10 ml
Size: 10 ml
This is one of a group of CaptureSelect™ protein purification affinity resins developed for the simple, single-step purification of a variety human plasma and serum proteins. This unique selection of affinity purification resins can purify proteins or other non-antibody biomolecules from either recombinant or native sources with a high degree of affinity and specificity, and are immobilized on a high capacity agarose support.
Catalog: 191306050
Size: 50 ml
Size: 50 ml
Sodium pyruvate is commonly added to cell culture media as an energy source in addition to glucose. Since cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not a required supplement for all cell cultures. However, if cells have been grown in medium that is supplemented with sodium pyruvate, we recommend continuing use of the supplement as cell growth may lag without it. Gibco® Sodium Pyruvate (100 mM) is formulated using 11 g per liter of water. The final concentration of sodium pyruvate used in most cell culture media is 1 mM. Dual-site cGMP Manufacturi...
Catalog: 11360-070
Size: 100 ml
Size: 100 ml
Dynabeads Insulin, Transferrin & Iron Supplements
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