Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Epitope Tag & Reporter Specific Antibodies
This purified Mouse Histidine (C-term) Tag Monoclonal Antibody recognizes a polyhistidine amino acid sequence at the carboxy-terminus of proteins. The free carboxy-terminus of the terminal histidine residue is a key element of the epitope recognition site of the anti-His (C-term) Antibody. The Anti-His(C-term) Monoclonal Antibody recognizes the sequence: -His-His-His-His-His-His-COOH . Validated applications for Histidine (C-term) Tag monoclonal antibody are western blotting and ELISA. Antibody Specifications: • Applications: Validated applications for Histidine (C-term) Tag Monoclonal Antibod...
Catalog: R931-25
Size: 50 µl
Category: Products & Services > Beads & Microspheres > Dynabeads® for Cell Isolation & Expansion > Dynabeads® for Other Cell Isolation
Dynabeads® Sheep-anti Rat IgG binds monoclonal rat IgGs of your choice for specific cell isolation or depletion. All cells from all species except from rat can be isolated with this product, depending only on the specific antibody of choice. Especially well suited for cell isolation from mouse samples. Depletion: cells can be depleted from a sample Positive isolation: cells can be positively isolated from a sample for molecular applications Negative isolation: cells can be negatively isolated from a sample (i.e. spleen or lymph node cells) by adding an antibody cocktail of rat IgGs towards the...
Catalog: 11035
Size: 5 ml
Category: Products & Services > Beads & Microspheres > Dynabeads® Surface-Activated
An excellent tool for highly predictable and consistent extraction and isolation of nucleic acids from biological samples, following a simple magnetic separation protocol. Please note that this is a stand-alone product containing only Dynabeads® in a storage buffer. Our products Dynabeads® SILANE genomic DNA (Cat.no. 370-12D) or Dynabeads® SILANE viral NA (Cat.no. 370-11D) include the appropriate buffers and protocols you need to extract genomic DNA or viral nucleic acids respectively. Magnetic particles from alternative suppliers often have a random size range distribution, surface area and b...
Catalog: 37002D
Size: 5 ml
Category: Products & Services > Beads & Microspheres > Dynabeads® for Cell Isolation & Expansion > Dynabeads® for Human Cell Isolation
Dynabeads® CD4 are magnetic beads that provide a robust and versatile tool to isolate or deplete human CD4 + T-cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. • Rapid isolation of CD4 + T-cells from any sample – no columns required • Positive isolation for molecular downstream assays or efficient depletion of CD4 + T cells Excellent Yield, Purity, and Viability of Recovered Cells Dynabeads® CD4 are uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of human CD4 + cells directly from any samp...
Catalog: 11145D
Size: 5 ml
Category: Products & Services > Beads & Microspheres > Dynabeads® Ligand-Coupled > Streptavidin-Coupled Dynabeads®
Streptavidin-coupled Dynabeads® is the gold standard for isolation and handling of biotinylated nucleic acids, antibodies or other biotinylated ligands and targets. The very high binding affinity of the streptavidin-biotin interaction (Kd=10 -15 ) is utilized in a vast number of applications. These uniform and superparamagnetic beads are 1 µm in diameter, with a monolayer - not a multilayer - of recombinant streptavidin covalently coupled to the surface and further blocked with BSA. The monolayer of streptavidin leaves the vast majority of the biotin binding sites sterically available for bind...
Catalog: 65602
Size: 10 ml
Category: Products & Services > Beads & Microspheres > Dynabeads® for Cell Isolation & Expansion > Dynabeads® for Human Cell Isolation
Enrich epithelial tumor cells directly from whole blood, bone marrow, or PBMC suspensions. Enriched tumor cells are lysed for mRNA isolation and RT-PCR amplification. The sensitivity of combined enrichment and RT-PCR detection can be as high as 1 tumor cell in 1 ml blood. Depletion of epithelial tumor cells from whole blood/bone marrow or PBMC. Positive isolation of epithelial tumor cells for downstream molecular analysis. No columns required Starting samples: Whole blood, bone marrow, PBMC, buffy coat. Number of cells processed: 200 ml whole blood or ~4 × 10 9 PBMC.
Catalog: 16102
Size: 5 ml
Category: Products & Services > Beads & Microspheres > Dynabeads® for Cell Isolation & Expansion > Dynabeads® for Human Cell Isolation
Use this kit to isolate a high purity and yield of human CD34+ progenitor stem cells. Positively isolated cells are bead and antibody-free, phenotypically unaltered and suitable for any downstream applications including flow cytometry, functional studies, and cell culture to produce dendritic cells (DC's). The Dynal® CD34 Progenitor Cell Isolation System can also be used to isolate CD34+ cells from rhesus monkey (Macaca mulatta). Positive isolation of human CD34+ progenitor stem cells with bead release Stem cells can be isolated directly from whole blood, cord blood or bone marrow Isolated CD3...
Catalog: 11301D
Size: 5 ml
Category: Products & Services > Labeling & Detection Products > Gel & Membrane Stains
InVision™ His-tag In-gel Stain is a ready-to-use, fast, sensitive and highly specific stain for visualizing His-tagged fusion protein bands directly in a polyacrylamide gel following electrophoresis. Using InVision™ His-tag In-gel Stain you'll: Stain proteins fused to an oligohistidine sequence Eliminate western blotting-detect His-tagged proteins directly in the gel Detect nanogram levels of protein bands with a UV transilluminator, equipped with a standard camera, or a laser-based scanner Visualize results in under 3 hours Convenienty provided as one ready-to-use solution, InVision™ His-tag ...
Catalog: LC6030
Size: 500 ml
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Magnets & Sample Mixers
Our Magnetic Particle Concentrators (MPC®) enable optimal binding of Dynabeads® magnetic beads with their targets (e.g., cells, proteins, and nucleic acids) in a variety of starting samples. The MPC®-S was designed specifically for microbiology applications; it has been validated with select Dynabeads® for enrichment of E. coli O157:H7, Salmonella, non-O157 STEC, Cryptosporidium, and Giardia. The MPC®-S can be used with both food enrichment samples types as well as water samples. It holds up to six microcentrifuge tubes and has an optimal working volume range of 20 µl–2 ml. Note that this magn...
Catalog: A13346
Size: 1 unit
Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Fluorescent Nucleic Acid Detection (FISH) Kits
FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor® colors or get the FISH Tag™ DNA Multicolor Kit , which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. FISH Tag™ Kit Specificatio...
Catalog: F32947
Size: 1 kit
Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Fluorescent Nucleic Acid Detection (FISH) Kits
The FISH Tag™ DNA Multicolor Kit provides a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. The FISH Tag™ DNA Multicolor Kit contains fluorescent dyes in four colors, making it ideal for simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. We also offer FISH Tag™ Kits in single colors. FISH Tag™ Kit Specificati...
Catalog: F32951
Size: 1 kit
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > Nuclear Receptors
Retinoid-related orphan receptor gamma, ligand binding domain (RORG-LBD) is a 58.60 kDa recombinant human protein (amino acids 250-518) expressed as a GST-tagged fusion protein in insect cells. RORG-LBD was expressed and purified in the absence of exogenous ligands, making it ideal for ligand binding and coregulator displacement studies.
Catalog: PV5888
Size: 50 μg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Cluster of Differentiation 44 (CD44), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Pro 220) of human CD44 (P16070-1) fused to a polyhistidine tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and ...
Catalog: 12211-H08H-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ephrin-B1 (EFNB1), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 232) of human EFNB1 (NP_004420.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized mouse EFNB3 at 2 µg/ml(100 µl/well) can bind human EFNB1-Fc with...
Catalog: 10894-H03H-25
Size: 25 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ephrin-B1 (EFNB1), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 232) of human EFNB1 (NP_004420.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized mouse EFNB3 at 2 µg/ml(100 µl/well) can bind human EFNB1-Fc with...
Catalog: 10894-H03H-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ephrin-B1 (EFNB1), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 232) of human EFNB1 (NP_004420.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized mouse EFNB3 at 2 µg/ml(100 µl/well) can bind human EFNB1-Fc with...
Catalog: 10894-H03H-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ephrin-B1 (EFNB1), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 232) of human EFNB1 (NP_004420.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized mouse EFNB3 at 2 µg/ml(100 µl/well) can bind human EFNB1-Fc with...
Catalog: 10894-H03H-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ephrin-B2 (EFNB2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ala 229) of human EFNB2 (NP_004084.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized human EFNB4-His at 2 µg/ml(100 µl/well)...
Catalog: 10881-H03H-25
Size: 25 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ephrin-B2 (EFNB2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ala 229) of human EFNB2 (NP_004084.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized human EFNB4-His at 2 µg/ml(100 µl/well)...
Catalog: 10881-H03H-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ephrin-B2 (EFNB2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ala 229) of human EFNB2 (NP_004084.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized human EFNB4-His at 2 µg/ml(100 µl/well)...
Catalog: 10881-H03H-5
Size: 5 µg