Dye Stains

InVision™ His-Tag In-Gel Stain

Category: Products & Services > Labeling & Detection Products > Gel & Membrane Stains

InVision™ His-tag In-gel Stain is a ready-to-use, fast, sensitive and highly specific stain for visualizing His-tagged fusion protein bands directly in a polyacrylamide gel following electrophoresis. Using InVision™ His-tag In-gel Stain you'll: Stain proteins fused to an oligohistidine sequence Eliminate western blotting-detect His-tagged proteins directly in the gel Detect nanogram levels of protein bands with a UV transilluminator, equipped with a standard camera, or a laser-based scanner Visualize results in under 3 hours Convenienty provided as one ready-to-use solution, InVision™ His-tag ...
Catalog: LC6030
Size: 500 ml

SYTO® 82 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains

The cell-permeant SYTO 82 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.
Catalog: S11363
Size: 250 µl

Trypan Blue Stain 0.4%, for use with the Countess® Automated Cell Counter

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

Trypan blue stain is a well-accepted method for staining dead cells. It can enter into cells with compromised membranes and is the method used with the Countess® automated cell counting platform.
Catalog: T10282
Size: 2 × 1 ml

SYTO® 60 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains

The cell-permeant SYTO 60 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.
Catalog: S11342
Size: 250 µl

SYTOX® Green Nucleic Acid Stain - 5 mM Solution in DMSO

Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains

SYTOX® Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population. • Impermeant to live cells • Excitation/Emission: 504/523 nm • Use with 488 Argon-ion laser • Works with mammalian cells and both Gram-positive and Gram-negative bacteria Simple Viability Determination SYTOX® Green allows quick determination of cell viability when using flow cytometers, fluorescence microscopes, fluorometers, or fluorescence microplate readers. It will not cross intact membranes, ...
Catalog: S7020
Size: 250 µl

HCS CellMask™ Red Stain

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

HCS CellMask™ Red stain (H32712) replaces the HCS CellMask™ Red cytoplasmic⁄nuclear stain (H32711) as a cell delineation tool for HCS platforms. HCS CellMask™ stains label the entire cell (cytoplasm and nucleus) and provide an accurate backdrop against which the features of interest can be assessed. HCS CellMask™ stains can be applied to cells immediately after fixation or in the last step of multiplexing protocols, and they are compatible with detergent-based cell permeabilization protocols. If only nuclear staining is desired, HCS NuclearMask™ stains can be used for measuring DNA content in ...
Catalog: H32712
Size: 1 set

Alkaline Phosphatase Live Stain

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

Alkaline Phosphatase Live Stain is a stem cell imaging product that allows users to differentially stain pluripotent stem cells (PSCs). The dye is a cell-permeable fluorescent substrate for alkaline phosphatase (AP) that is non-toxic to cells, diffusing out over the course of two hours. The Alkaline Phosphatase Live Stain consists of 50 µL of a 500X fluorescein-based dye in DMSO, sufficient for staining two 24-well plates, four 6 cm dishes or two 10 cm dishes. This Alkaline Phosphatase Live Stain allows you to: • Identify pluripotent stem cells with a strong signal above background • Stain PSC...
Catalog: A14353
Size: 50 µl

Pro-Q® Emerald 300 Lipopolysaccharide Gel Stain Kit

Category: Products & Services > Labeling & Detection Products > Gel & Membrane Stains

Molecular Probes' proprietary Pro-Q Emerald 300 and Pro-Q Emerald 488 Glycoprotein Stain Kits provide the most advanced technology available for detection of glycoproteins in gels and on blots. Gel staining is rapid and very sensitive. In less than three hours, it is possible to detect as little as 300 pg of glycoprotein per band, depending on the degree of glycosylation, making these stains at least 50-fold more sensitive than standard fuchsin staining.
Catalog: P-20495
Size: 1 kit

HCS LipidTOX™ Green Neutral Lipid Stain, for cellular imaging

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Green neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid s...
Catalog: H34475
Size: 1 each

StainEase® Staining Trays

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Gel Blotting, Drying & Staining Accessories

The StainEase¤ Gel Staining Tray is a convenient way to evenly stain mini-gels and prevent gels from breaking. The staining tray assembly includes a perforated strainer insert that holds the gel and drains away the stain, a lid, and an outer container in which stains and solutions can be changed without having to handle the gel. Easily fits up to four mini-gels.
Catalog: NI2400
Size: 2 packs

CellMask™ Deep Red Plasma membrane Stain

Category: Products & Services > Labeling & Detection Products > Cell Stains > Organelle Stains

The CellMask™ Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins. These stains may be used as a segmentation tool for HCS (high-content screening), as well as to stain cellular plasma membranes for standard fluorescence microscopy. The stain survives fixation, but not permeabilization, so it is not suitable for experiments that also involve probing internal targets via antibodies.
Catalog: C10046
Size: 100 µl

ROX Reference Dye

Category: Products & Services > Labeling & Detection Products > Fluorescent Dyes

ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR. This dye has been qualified for use on the ABI PRISM® 7700 with Platinum® Quantitative PCR SuperMix-UDG® , Platinum® Quantitative RT-PCR ThermoScript™ One-Step System, and SuperScript® III Platinum® One-Step qRT-PCR Kit. ROX Reference Dye: Normalizes non-PCR-related fluctuations in fluorescence Provides a stable baseline for multiplex quantitative PCR and RT-PCR Uses existing ROX spectral settings, eliminating the need to re-calibrate the thermal cycler
Catalog: 12223-012
Size: 500 µl

FM® 4-64FX, fixable analog of FM® 4-64 membrane stain

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

The lipophilic probe, FM™ 4-64FX exhibits low fluorescence in water but fluoresces intensely upon binding the outer leaf of the plasma membrane providing discrete plasma membrane staining. The binding is rapid and reversible and "FX" denotes that this analog is fixable with aldehyde-based fixatives. It is also an excellent reagent for identifying actively firing neurons and for investigating the mechanisms of activity-dependent vesicle cycling.
Catalog: F34653
Size: 10 × 100 µg

FxCycle™ PI/RNase Staining Solution

Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains

FxCycle™ PI/RNase Staining Solution is used for flow cyotmetric analysis of DNA content in fixed cells. Propidium iodide (PI), a popular red-fluorescent stain, binds to DNA by intercalating between the bases with little or no sequence preference and with a stoichiometry of one dye per 4–5 base pairs of DNA. PI also binds to RNA, necessitating treatment with RNase to distinguish between RNA and DNA staining. This propidium iodide solution includes DNase-free RNase A and a permeablization reagent. It comes ready to use: simply add the staining solution to fixed cells, incubate, and acquire on a ...
Catalog: F10797
Size: 100 ml

HCS LipidTOX™ Red Neutral Lipid Stain, for cellular imaging

Category: Products & Services > Kits & Assays > Cell Analysis Kits > HCS Detection Kits

The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Red neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid sta...
Catalog: H34476
Size: 1 each

DS-30 Matrix Standard Kit (Dye Set D)

Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > DNA Sequencing & Matrix Standards

The DS-30 Matrix Standard kit contains 4 oligonucleotides respectively labeled with 6-FAM™, HEX™, NED™, and ROX™, pre-pooled in a single tube. This Matrix Standard Set is used to generate the "multicomponent matrix" required when analyzing fluorescent dye-labeled DNA fragments on the Applied Biosystems® 3100, 3130, and 3730 Series Systems. The Data Collection Software for these instruments uses the multicomponent matrix to automatically analyze the four different colored fluorescent dye-labeled samples in a single capillary. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4345827
Size: 8 runs

FISH Tag™ DNA Green Kit, with Alexa Fluor® 488 dye

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Fluorescent Nucleic Acid Detection (FISH) Kits

FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor® colors or get the FISH Tag™ DNA Multicolor Kit , which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. FISH Tag™ Kit Specificatio...
Catalog: F32947
Size: 1 kit

6-FAM™ Dye Phosphoramidite, 45 mg

Category: Products & Services > Buffers & Chemicals > General Chemicals > Oligo Synthesis Reagents

Contains 45 mg 6-FAM™ Dye Phosphoramidite. 6-FAM™ Dye Phosphoramidite is used for the 5’ attachment of FAM™ dye during oligonucleotide synthesis. 6-FAM™ Dye Phosphoramidite is an optically pure isomer (not a mixture). The label is treated as if it were simply another base: It is inserted at the appropriate place in the sequence and incorporated automatically. • Specialty phosphoramidites allow online addition of the label during synthesis, saving time in production of labeled probes and primers • Designed and optimized for use with ABI™ synthesizers, guaranteeing reliable nucleic acid synthesi...
Catalog: 403169
Size: 45 mg

FISH Tag™ DNA Multicolor Kit, Alexa Fluor® dye combination

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Fluorescent Nucleic Acid Detection (FISH) Kits

The FISH Tag™ DNA Multicolor Kit provides a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. The FISH Tag™ DNA Multicolor Kit contains fluorescent dyes in four colors, making it ideal for simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. We also offer FISH Tag™ Kits in single colors. FISH Tag™ Kit Specificati...
Catalog: F32951
Size: 1 kit

LIVE/DEAD® Fixable Blue Dead Cell Stain Kit, for UV excitation

Category: Products & Services > Kits & Assays > Cell Analysis Kits > Cell Viability Kits

The LIVE/DEAD® Fixable Dead Cell Stain Kit employs an amine-reactive fluorescent dye to evaluate mammalian cell viability by flow cytometry. In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity between the live and dead cell populations is typically greater than 50 fold, and this fluorescence intensity discrimination is completely preserved fol...
Catalog: L-23105
Size: 1 kit