Dot Blot
Unconjugated polyclonal antibody specific to human H3K27me2. This antibody is validated for use in Chromatin Immunoprecipitation (ChIP), ELISA, dot blotting and Western blotting. This antibody has been raised against the region of the histone H3 containing the dimethylated lysine 27 (H3K27me2), using a KLH-conjugated synthetic peptide. Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histo...
Catalog: 49-1013
Size: 50 µg
Size: 50 µg
Replacement Parts for Electrophoresis Apparatus
Catalog: EI9052
Size: each
Size: each
Invitrogen makes blotting of E-PAGE™ Gels easier and more efficient by providing a pre-cut fiber blotting pad and several types of pre-cut, pre-assembled membrane/filter paper sandwiches that perfectly fit 7.5 cm x 8.4 cm E-PAGE™ Gels*. The available nitrocellulose and Invitrolon™ PVDF membranes are designed for western transfer of proteins (Figure 1, Table 1). Pre-cut, extra-thick, absorbent Blotting Paper (2.5 mm thickness) for E-PAGE" Gels are also available to accommodate the E-PAGE™ blotting set-up using a semi-dry transfer apparatus. A protein s properties affect its ability to bind to m...
Catalog: LC2010
Size: 50 sheets
Size: 50 sheets
The XCell II™ Blot Module allows you to easily transfer proteins or nucleic acids from mini-gels to membranes. It fits neatly into the XCell SureLock ™ and XCell II™ Mini-Cells in place of the gel/buffer core assembly. It requires less than 200 ml of transfer buffer for western, Southern, and northern transfers. Tough platinized titanium and stainless steel electrodes create a uniform electrical field without clamps or hinged gel holders. Maximum blot size is 9 cm x 9 cm.
Catalog: EI9051
Size: 1 unit
Size: 1 unit
Invitrogen makes blotting of E-PAGE™ Gels easier and more efficient by providing a pre-cut fiber blotting pad and several types of pre-cut, pre-assembled membrane/filter paper sandwiches that perfectly fit 8.6 cm x 13.5 cm E-PAGE™ Gels*. The available nitrocellulose and Invitrolon™ PVDF membranes are designed for western transfer of proteins (Figure 1, Table 1). Pre-cut, extra-thick, absorbent Blotting Paper (2.5 mm thickness) for E-PAGE" Gels are also available to accommodate the E-PAGE™ blotting set-up using a semi-dry transfer apparatus. A protein s properties affect its ability to bind to ...
Catalog: LC2101
Size: 4 pads
Size: 4 pads
The Blotting Roller is an 8.6 cm wide roller designed for easy and efficient removal of bubbles when assembling gel/membrane sandwiches for blotting both E-PAGE™ Gels and mini-gels such as NuPAGE® and Novex¤ Tris-Glycine Gels. It consists of a Delrin roller attached to a stainless steel handle. The Incubation Tray enables convenient and even staining of E-PAGE™ blots. The tray is 10 cm (l) x 14 cm (w) x 3 cm (d) with a lid. It is suitable for use with immunodetection or blot stains.
Catalog: LC2100
Size: 1 unit
Size: 1 unit
The XCell line of mini-cells has always distinguished itself by being simple, sturdy, and convenient. The tradition continues with the patented XCell SureLock ® Mini-Cell. The incorporation of a gel tension wedge in place of the rear wedge used on earlier models makes the XCell SureLock ® the easiest-to-use mini-cell on the market. How the System Works The XCell SureLock ® Mini-Cell holds gels firmly with a simple gel tension wedge. When the lever on the gel tension wedge is pushed forward into the locked position, an even horizontal force is generated. This seals the gel/buffer core assembly ...
Catalog: EI0002
Size: 1 unit
Size: 1 unit
The Blotting Tweezer is a replacement part for the Bolt™ Mini Blot Module. It is for general use of handling nitrocellulose or PVDF membranes. About the Bolt™ Mini Blot Module The Bolt™ Mini Blot Module is a wet transfer device used exclusively with the Bolt™ Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Bolt™ Mini Blot Module is compatible with Bolt™ Bis-Tris Plus gels and accommodates one gel per wet transfer. The universal connection and molded gasket make the blot module easy to use, while the inner core of the...
Catalog: B1002
Size: 1 unit
Size: 1 unit
The Bolt® Mini Blot Module Gasket is a replacement part for the Bolt® Mini Blot Module. The gasket fits on the cathode core (-) of the blot module. About the Bolt® Mini Blot Module The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Bolt® Mini Blot Module is compatible with Bolt® Bis-Tris Plus gels and accommodates one gel per wet transfer. The universal connection and molded gasket make the blot module easy to use, while the inner core o...
Catalog: B1001
Size: 1 unit
Size: 1 unit
The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Bolt® Mini Blot Module is compatible with Bolt® Bis-Tris Plus gels and accommodates one gel per wet transfer. The universal connection and molded gasket make the blot module easy to use, while the inner core of the blot module allows for use of less methanol-based transfer buffer per wet transfer. At the recommended conditions and constant voltage, proteins can be transferred to nitrocel...
Catalog: B1000
Size: 1 unit
Size: 1 unit
This concentrated Nitro-Block-II™ Enhancer can be used with CSPD® or CDP-Star® chemiluminescent substrates to increase the alkaline phosphatase signal for protein or nucleic acid blots. The Nitro-Block-II™ membrane enhancer increases chemiluminescent signal intensity on nitrocellulose and PVDF membranes. • Chemically optimized formulations provide enhanced signal intensity in blotting applications with dioxetane-based substrate solutions (CSPD® or CDP-Star® substrates) on nitrocellulose or PVDF membranes. • Enhancer increases signal intensity, allowing decreased signal acquisition time. Increa...
Catalog: T2184
Size: 20 ml
Size: 20 ml
TMB (Tetramethyl Benzidine) chromogen solution may be used with horseradish peroxidase (HRP)-based immunodetection procedures.
Catalog: 00-2019
Size: 500 ml
Size: 500 ml
Denhardt's Solution is a mixture of blocking agents used in membrane-based hybridization protocols. The solution contains 1% Ficoll (type 400), 1% polyvinylpyrrolidone, and 1% bovine serum albumin. Sterile filtered.
Catalog: 750018
Size: 100 ml
Size: 100 ml
The iBlot® Gel Transfer Device performs western blotting transfer efficiently and reliably typically in 7 minutes or less without the need for liquid buffers. The device is integral part of the iBlot® Western Blotting System. The system consists of the device and disposable blotting stacks that contain the buffers and the transfer membrane (nitrocellulose or PVDF), and offers high quality transfer, convenience and speed. The device and the stacks are sold separately. The iBlot® system is ideal for scientists analyzing proteins through western blotting who want: • Complete protein transfer in s...
Catalog: IB1001
Size: 1 each
Size: 1 each
The amine-reactive Lissamine™ rhodamine B sulfonyl chloride can be used to create red-fluorescent bioconjugates with excitation/emission maxima ~568/583 nm.
Catalog: L-20
Size: 1 g
Size: 1 g
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
Catalog: C-6164
Size: 10 mg
Size: 10 mg
DryEase® Mini-Gel Cellophane is one component of the DryEase® Mini-Gel Drying System , which is a simple, easy-to-use kit for drying gels evenly using passive evaporation. The kit allows gels to be assembled on a base between two open frames, so that both outer surfaces of the gels are exposed to air. Consequently, drying is even and cracking is minimized. The proprietary Gel-Dry™ Drying Solution modulates the rate of water release to ensure crack-free gels. With the DryEase® System, gels are dried overnight with uniform tension on all points of the gel to eliminate cracking. Applications: Gel...
Catalog: NC2380
Size: 200⁄pack
Size: 200⁄pack
Invitrogen makes blotting of E-PAGE™ Gels easier and more efficient by providing a pre-cut fiber blotting pad and several types of pre-cut, pre-assembled membrane/filter paper sandwiches that perfectly fit 8.6 cm x 13.5 cm E-PAGE™ Gels*. The available nitrocellulose and Invitrolon™ PVDF membranes are designed for western transfer of proteins (Figure 1, Table 1). Pre-cut, extra-thick, absorbent Blotting Paper (2.5 mm thickness) for E-PAGE" Gels are also available to accommodate the E-PAGE™ blotting set-up using a semi-dry transfer apparatus. A protein s properties affect its ability to bind to ...
Catalog: LC2007
Size: 16⁄pack
Size: 16⁄pack
The StainEase¤ Gel Staining Tray is a convenient way to evenly stain mini-gels and prevent gels from breaking. The staining tray assembly includes a perforated strainer insert that holds the gel and drains away the stain, a lid, and an outer container in which stains and solutions can be changed without having to handle the gel. Easily fits up to four mini-gels.
Catalog: NI2400
Size: 2 packs
Size: 2 packs
For the preparation and running of agarose gels. For use with RNA denatured by glyoxal treatment; one bottle containing 1 L is provided. Ambion® NorthernMax® reagents are the same components included in the NorthernMax® Ultrasensitive Northern Blotting Kits but in larger sizes. These reagents are supplied ready to use and are certified RNase-free. Comprehensive instructions are included.
Catalog: AM8678
Size: 1 L
Size: 1 L
Dot Blot
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