Dnase⁄Rnase-Free Reagents

UltraPure™ DNAse/RNAse-Free Distilled Water

Category: Products & Services > Buffers & Chemicals > Water

UltraPure™ DNase/RNase-Free Distilled Water is designed for use in all molecular biology applications. It is 0.1-µm membrane-filtered and tested for DNase, RNase, and protease activity.
Catalog: 10977035
Size: 500 ml

RNase AWAY ™ Reagent

Category: Products & Services > Buffers & Chemicals > Nucleic Acid Storage & Nuclease Control Reagents > Nuclease Control Reagents

RNase AWAY® Reagent is a ready-to-use solution for eliminating RNase and DNA contamination from labware. Apply it evenly over the surface of glassware or plasticware to be treated and then rinse it away with distilled water. Unwanted RNase and DNA contamination are eliminated. RNase AWAY® Reagent is nonabrasive, noncarcinogenic, and nonbiologically corrosive. Performance and Quality Testing: RNase AWAY® Reagent is functionally tested for the elimination of RNase activity and DNA. No detectable RNase activity or DNA is observed.
Catalog: 10328-011
Size: 250 ml

20X TE Buffer, RNase-free

Category: Products & Services > Buffers & Chemicals > General Buffers > Tris & TE Buffers

The RNase-free TE buffer concentrate, 20X TE buffer , which is essential to the success of the RiboGreen® RNA quantitation assays (R11491, R37302) is available separately from the kits to extend the number of low-concentration assays.
Catalog: T11493
Size: 100 ml

RNase-free Microfuge Tubes (1.5 ml)

Category: Products & Services > Instruments, Software, & Accessories > Tips, Tubes, Strips & Accessories > Other Tubes

Ambion® RNase-free Microfuge Tubes are guaranteed RNase- and DNase-free and have a positive seal design that withstands –90° to 120°C. They are marked with convenient graduations. 1.5 mL tubes are provided in a box of 500. Each lot of tubes is certified RNAse- and DNase-free.
Catalog: AM12400
Size: 500 tubes

Non-Stick RNase-free Microfuge Tubes (1.5 ml)

Category: Products & Services > Instruments, Software, & Accessories > Tips, Tubes, Strips & Accessories > Other Tubes

Ambion® Non-Stick RNase-free Microfuge Tubes have a low binding surface and are guaranteed RNase- and DNase-free. They have a positive seal design that withstands –90° to 120°C and are marked with convenient graduations. 1.5 mL tubes are provided in a box of 250. Each lot of tubes is certified RNAse- and DNase-free.
Catalog: AM12450
Size: 250 tubes

PFHM-II Protein-Free Hybridoma Medium

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Hybridoma Media

Gibco® Protein Free Hybridoma Medium II (PFHM II) is a serum-free, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® PFHM II features: • Ability to support suspension and stationary hybridomas and myelomas • Complete, ready-to-use formulation • Serum-free, protein-free formulation Ability to support suspension and stationary hybridomas and myelomas Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® PFHM II provides high cell densities and IgG production. Cholesterol-depende...
Catalog: 23600042
Size: 1 x 10L

RNase Zap ®

Category: Products & Services > Buffers & Chemicals > Nucleic Acid Storage & Nuclease Control Reagents > Nuclease Control Reagents

Ambion® RNaseZap® is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap® onto the surface to be decontaminated and rinse it off with RNase-free water. Supplied in a single 4 liter cubitainer. • Completely removes RNase contamination from glass and plastic surfaces • Excels at removing high levels of RNase contamination whereas similar products fail • Proven effective at removing high concentrations of dried-on RNase A • Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-free Working with RNA requires that special measures be...
Catalog: AM9784
Size: 4 L

7300 & 7500 Real Time PCR Systems TaqMan®RNase P 96-Well Instrument Verification Plate

Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > Real-Time PCR Instrument Calibration Kits & Reagents

The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is used to verify the performance of the Applied Biosystems® 7300 and 7500 Real-Time PCR Systems. The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is a 96-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template. T...
Catalog: 4350584
Size: 1 kit

Cell Dissociation Buffer, enzyme-free, PBS

Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Cell Dissociation Buffers

Gibco® Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Phosphate Buffered Saline (PBS). Gibco® Cell Dissociation Buffer is suitable for gentle dissociation of mammalian cells for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry. Gibco® Cell Dissociation Buffer works well with lightly adherent cell lines such as HeLa and NIH 3T3, but is not recommended for routine passage of strongly adherent cells. The dis...
Catalog: 13151-014
Size: 100 ml

Fixative-Free Lysing Solution, High-Yield Lyse

Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents

High-Yield Lyse is a premixed, fixative-free erythrocyte lysing solution that can be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. Using this reagent, lysis of erythrocytes is performed immediately following staining of the blood samples with Invitrogen's fluorochrome-conjugated monoclonal antibodies.
Catalog: HYL-250
Size: 500 ml

TaqMan® RNase P Control Reagents Kit

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

The RNase P gene is a single-copy gene that encodes the RNA moiety for the RNase P enzyme. Applied Biosystems® TaqMan® RNase P Control Reagents were designed with limiting primer concentrations to be used as the endogenous reference in multiplex reactions. Multiplex PCR is the use of more than one probe and primer pair in the same tube. This kit is designed to perform a 5' nuclease assay with TaqMan® Universal PCR Master Mix using the provided human genomic DNA as template. • Pre-designed primers and TaqMan® probe eliminate assay design • Easy-to-use primer and TaqMan® probe mix minimizes assa...
Catalog: 4316844
Size: 1000 reactions

Hepatocyte Thaw Media

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Other Serum-Free & Specialty Media

Gibco® Hepatocyte Thaw Media is a ready-to-use media specifically designed for use with thawed cryopreserved hepatocytes to enhance their viability and overall quality. This media is provided in a 50 mL conical tube for ease of use. Features of Hepatocyte Thaw Media: • Easy to use—simply warm up to 37°C and add thawed hepatocytes • Recover cryopreserved hepatocytes with a single centrifugation • Designed for use with Gibco® human and animal cryopreserved hepatocytes for optimal results • Provides high quality hepatocytes suitable for many in vitro assays, including induction, metabolic stabili...
Catalog: CM7500
Size: 50 ml

Human Endothelial-SFM

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Other Serum-Free & Specialty Media

Human Endothelial-SFM is a basal serum-free growth medium developed to support the isolation and expansion of human umbilical venous and arterial endothelial cells (1–4). Medium requires supplementation with bFGF (20 ng/ml), EGF (10 ng/ml), and human plasma fibronectin (10 µg/ml). Contains L-glutamine.
Catalog: 11111-044
Size: 500 ml

Macrophage-SFM, (1X), Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Other Serum-Free & Specialty Media

Macrophage-SFM is a serum-free medium designed for the culture of human peripheral blood monocytes and macrophages (1). Contains L-glutamine, but no antibiotics.
Catalog: 12065-074
Size: 500 ml

CD CHO Medium (1X), Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > CHO & DG44 Media

CD CHO medium is a protein-free, serum-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture. With a proven track record of quality for more than 10 years, CD CHO medium contains no proteins or peptide components of animal, plant, or synthetic origin, as well as no undefined lysates or hydrolysates. When to Use CD CHO Medium • Your GS CHO cells are recommended to be grown in CD CHO medium • Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a reg...
Catalog: 10743-029
Size: 1,000 ml

StemPro®-34 SFM (1X), Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Stem Cell Media

StemPro®-34 SFM is a serum-free medium specifically formulated to support the development of human hematopoietic cells in culture (1). StemPro®-34 SFM can be used to study hematopoietic cells from bone marrow, peripheral blood, or neonatal cord blood. StemPro®-34 SFM is performance-tested on normal human bone marrow. It is manufactured with components of human, recombinant or synthetic origin. StemPro®-34 SFM consists of a basal liquid medium and frozen supplement (StemPro®-Nutrient Supplement) which is added at the time of use.
Catalog: 10639-011
Size: 500 ml

Keratinocyte-SFM Medium (Kit) with L-Glutamine, EGF and BPE

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Keratinocyte-SFM

with L-Glutamine, EGF and BPE. Shelf life after supplementation: 2 weeks
Catalog: 17005-075
Size: 1 kit

Essential 8™ Medium (prototype)

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Stem Cell Media

Essential 8™ Medium (published as E8) is a xeno-free and feeder-free medium specially formulated for the growth and expansion of human pluripotent stem cells (PSCs). Originally developed by Guokai Chen et al. (1) in the laboratory of James Thomson (published as "E8") and validated by Cellular Dynamics International, Essential 8™ Medium has been extensively tested and is proven to maintain pluripotency in multiple iPSC lines. In addition, Essential 8™ Medium has been used to scale up production of iPSCs and has been shown to support iPSC growth for >50 passages without any signs of karyotypic a...
Catalog: A14666SA
Size: 500 ml

CD Hybridoma Medium (1X), Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Hybridoma Media

Gibco® CD Hybridoma Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® CD Hybridoma Medium features: • Ability to support suspension and stationary hybridomas and myelomas • Formulated without L-glutamine for stability • Animal origin-free, protein-free and chemically defined formulation Ability to support suspension and stationary hybridomas and myelomas Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® CD Hybridoma Medi...
Catalog: 11279-023
Size: 1,000 ml

Gibco® FreeStyle™ 293 Expression Medium

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > 293 Media

FreeStyle™ 293 Expression Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth and transfection of 293-F cells in suspension without adaptation. The medium is complete and ready-to-use, with no supplementation required. Gibco® FreeStyle™ 293 Expression Medium features: • Complete and ready-to-use format containing GlutaMAX™ supplement • Ability to support suspension growth and transfection of 293-F cells • Animal origin-free, protein-free and chemically defined formulation • Easy use as part of the FreeStyle™ 293 ...
Catalog: 12338-018
Size: 1000 ml