Dnase⁄Rnase-Free Distilled Water

UltraPure™ DNAse/RNAse-Free Distilled Water

Category: Products & Services > Buffers & Chemicals > Water

UltraPure™ DNase/RNase-Free Distilled Water is designed for use in all molecular biology applications. It is 0.1-µm membrane-filtered and tested for DNase, RNase, and protease activity.
Catalog: 10977035
Size: 500 ml

Distilled Water

Category: Products & Services > Buffers & Chemicals > Water

Gibco® Distilled Water is high quality water for use as a solvent in the preparation of cell culture media and laboratory reagents. Available in many sizes to meet your needs, Gibco® Distilled Water features: • Cell culture quality • Proven performance • Use in cell culture and molecular biology applications Cell culture quality Gibco® Distilled Water is prepared by distillation and filtration (0.1 µm), with no added substances. Life Technologies monitors the water quality on a routine basis. Conductivity, total organic carbon, endotoxin and bioburden are tested daily; metals are tested monthl...
Catalog: 15230-071
Size: 100 ml

20X TE Buffer, RNase-free

Category: Products & Services > Buffers & Chemicals > General Buffers > Tris & TE Buffers

The RNase-free TE buffer concentrate, 20X TE buffer , which is essential to the success of the RiboGreen® RNA quantitation assays (R11491, R37302) is available separately from the kits to extend the number of low-concentration assays.
Catalog: T11493
Size: 100 ml

RNase-free Microfuge Tubes (1.5 ml)

Category: Products & Services > Instruments, Software, & Accessories > Tips, Tubes, Strips & Accessories > Other Tubes

Ambion® RNase-free Microfuge Tubes are guaranteed RNase- and DNase-free and have a positive seal design that withstands –90° to 120°C. They are marked with convenient graduations. 1.5 mL tubes are provided in a box of 500. Each lot of tubes is certified RNAse- and DNase-free.
Catalog: AM12400
Size: 500 tubes

Non-Stick RNase-free Microfuge Tubes (1.5 ml)

Category: Products & Services > Instruments, Software, & Accessories > Tips, Tubes, Strips & Accessories > Other Tubes

Ambion® Non-Stick RNase-free Microfuge Tubes have a low binding surface and are guaranteed RNase- and DNase-free. They have a positive seal design that withstands –90° to 120°C and are marked with convenient graduations. 1.5 mL tubes are provided in a box of 250. Each lot of tubes is certified RNAse- and DNase-free.
Catalog: AM12450
Size: 250 tubes

Nuclease-Free Water (not DEPC-Treated) (1 x 500 mL)

Category: Products & Services > Buffers & Chemicals > Water

Ambion® Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. It is ready to use and requires no preparation, mixing, or autoclaving. Because it is not DEPC treated, it is recommended for applications for which residual DEPC might prove detrimental (e.g. oocyte microinjection). This nuclease-free water is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
Catalog: AM9930
Size: 1 × 500 ml

PFHM-II Protein-Free Hybridoma Medium

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Hybridoma Media

Gibco® Protein Free Hybridoma Medium II (PFHM II) is a serum-free, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® PFHM II features: • Ability to support suspension and stationary hybridomas and myelomas • Complete, ready-to-use formulation • Serum-free, protein-free formulation Ability to support suspension and stationary hybridomas and myelomas Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® PFHM II provides high cell densities and IgG production. Cholesterol-depende...
Catalog: 23600042
Size: 1 x 10L

RT-PCR Grade Water (10 x 1.5 mL)

Category: Products & Services > Buffers & Chemicals > Water

Ambion® RT-PCR grade water is tested for prokaryotic as well as eukaryotic genomic DNA contamination using an ultrasensitive PCR assay. It is provided in 10 x 1.5 mL. Prokaryotic DNA contamination is assessed by performing PCR with primers to 16S rRNA. Eukaryotic DNA contamination is assessed by performing PCR with primers to 18S rRNA. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity and is suitable for any PCR application.
Catalog: AM9935
Size: 10 × 1.5 ml

Covaris® M220 AFA-grade Water

Category: Products & Services > Buffers & Chemicals > Water

Highly purified water (at least ASTM Type III or ISO Grade 3) is required when using the M220 Focused-ultrasonicator™. One AFA-grade water bottle (800 ml) is sufficient for more than 50 uses.
Catalog: 4482280
Size: 1 each

TaqMan® Copy Number Reference Assay, RNase P, Human

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

Human TaqMan® Copy Number Reference Assays are run with human TaqMan® Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome. Two options for genes that can be used as endogenous reference genes in humans are offered: RNase P and TERT. TaqMan® Copy Number Reference Assays are designed to unique genomic sequences in the reference genome assembly (NCBI Build 37/hg19) and are required for relative quantitation of copy number targets. TaqMan® Copy Number Assays quantitate the gene of interest and normalize t...
Catalog: 4403326
Size: 750 reactions

TaqMan® RNase P Control Reagents Kit

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

The RNase P gene is a single-copy gene that encodes the RNA moiety for the RNase P enzyme. Applied Biosystems® TaqMan® RNase P Control Reagents were designed with limiting primer concentrations to be used as the endogenous reference in multiplex reactions. Multiplex PCR is the use of more than one probe and primer pair in the same tube. This kit is designed to perform a 5' nuclease assay with TaqMan® Universal PCR Master Mix using the provided human genomic DNA as template. • Pre-designed primers and TaqMan® probe eliminate assay design • Easy-to-use primer and TaqMan® probe mix minimizes assa...
Catalog: 4316844
Size: 1000 reactions

TURBO DNA- free ™

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > DNA⁄RNA Modifying Enzymes

Ambion® TURBO DNA- free ™ contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. The kit includes sufficient reagents for 50 rxns. Features of Ambion® TURBO DNA- free ™ include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can plague RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The Best Method for Genomic DNA Removal Prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNa...
Catalog: AM1907
Size: 50 reactions

RNase Zap ®

Category: Products & Services > Buffers & Chemicals > Nucleic Acid Storage & Nuclease Control Reagents > Nuclease Control Reagents

Ambion® RNaseZap® is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap® onto the surface to be decontaminated and rinse it off with RNase-free water. Supplied in a single 4 liter cubitainer. • Completely removes RNase contamination from glass and plastic surfaces • Excels at removing high levels of RNase contamination whereas similar products fail • Proven effective at removing high concentrations of dried-on RNase A • Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-free Working with RNA requires that special measures be...
Catalog: AM9784
Size: 4 L

RNase AWAY ™ Reagent

Category: Products & Services > Buffers & Chemicals > Nucleic Acid Storage & Nuclease Control Reagents > Nuclease Control Reagents

RNase AWAY® Reagent is a ready-to-use solution for eliminating RNase and DNA contamination from labware. Apply it evenly over the surface of glassware or plasticware to be treated and then rinse it away with distilled water. Unwanted RNase and DNA contamination are eliminated. RNase AWAY® Reagent is nonabrasive, noncarcinogenic, and nonbiologically corrosive. Performance and Quality Testing: RNase AWAY® Reagent is functionally tested for the elimination of RNase activity and DNA. No detectable RNase activity or DNA is observed.
Catalog: 10328-011
Size: 250 ml

High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits

The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of input total RNA in a single 20 µL reaction to single stranded cDNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Includes sufficient reagents for 1000 x 20 µL reactions. • Linear target amplification for real-time PCR • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost • 10-fold greater dynamic range than other kits cDNA from Total RNA The High Capacity cDNA Reverse Transcription Kit (for...
Catalog: 4374967
Size: 1000 reactions

Nigericin, Free Acid

Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores

Intracellular calibration of the fluorescence response of cytosolic pH indicators is typically performed using the K + /H + ionophore nigericin , which causes equilibration of intracellular and extracellular pH in the presence of a depolarizing concentration of extracellular K + .
Catalog: N-1495
Size: 10 mg

Cell Dissociation Buffer, enzyme-free, PBS

Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Cell Dissociation Buffers

Gibco® Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Phosphate Buffered Saline (PBS). Gibco® Cell Dissociation Buffer is suitable for gentle dissociation of mammalian cells for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry. Gibco® Cell Dissociation Buffer works well with lightly adherent cell lines such as HeLa and NIH 3T3, but is not recommended for routine passage of strongly adherent cells. The dis...
Catalog: 13151-014
Size: 100 ml

Fixative-Free Lysing Solution, High-Yield Lyse

Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents

High-Yield Lyse is a premixed, fixative-free erythrocyte lysing solution that can be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. Using this reagent, lysis of erythrocytes is performed immediately following staining of the blood samples with Invitrogen's fluorochrome-conjugated monoclonal antibodies.
Catalog: HYL-250
Size: 500 ml

Water Saturated Phenol, pH 6.6 (Raise to pH 7.9 with included Buffer)

Category: Products & Services > Buffers & Chemicals > General Chemicals > Other General Chemicals

Ambion® Water Saturated Phenol is supplied premixed and at pH 6.6 (pH 7.9 ± 0.2, with included buffer). Water Saturated Phenol is used in RNA and DNA extraction procedures and is provided in one bottle of 400 mL. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality tested, saturated phenols are ready-to-use, eliminating handling problems and offering a more convenient, safer, and easier alternative to preparing solutions from crystalline phenol.
Catalog: AM9712
Size: 400 ml

RNase V1 (0.1 U/μl)

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > DNA⁄RNA Modifying Enzymes

Optimized for researchers performing RNA structure, RNA sequencing, protein footprinting, and boundary experiments, Ambion® RNase V1, RNA-Grade cleaves base-paired nucleotides and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites. Supplied in one tube of 200 µl (0.1 U/ µL); optimization reagents also included. In addition to applications for RNA structural analysis, RNA-Grade ribonucleases can be used to map protein binding sites on RNAs by comparing cleavage patterns in the presence and absence of an RNA binding p...
Catalog: AM2275
Size: 200 µl