DNA Sequencing Pcr
The TOPO® TA Cloning® Kits for Sequencing provide a highly efficient, 5 minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing. Each kit uses the pCR™4-TOPO® TA vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction. These kits include everything necessary to clone and select recombinant vectors containing your PCR fragment of choice. Get More Sequence— Allows for more insert sequence and less vector seuquence when usin...
Catalog: K4530-20
Size: 20 reactions
Size: 20 reactions
Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 ml of 50 mM MgCl 2 . It is included with Platinum® Taq, Taq, and the SuperScript® First-Strand Synthesis System for RT-PCR.
Catalog: 18067-017
Size: 2 × 1 ml
Size: 2 × 1 ml
The BigDye® Terminator v3.1 sequencing standard kit contains four tubes, each of which contains enough DNA of a known 1200 base pair sequence prepared with BigDye® Terminator v3.1 to perform one spectral calibration⁄performance check and⁄or one control sequencing run on the Applied Biosystems® 3500 Series Genetic Analyzer. The DNA contained in the preparation has been lyophilized to maximize stability. For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Catalog: 4404312
Size: 4 tubes
Size: 4 tubes
Zero Blunt® TOPO® PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR®-Blunt II-TOPO® Vector for 5-minute benchtop ligations without ligase (1). The pCR®-Blunt II-TOPO® Vector also features: • ccdB gene for positive selection (2) • Eco R I sites flanking the PCR product insertion site for easy excision of inserts • Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli • SP6 promoter/primer site for...
Catalog: K2800-02
Size: 20 preps
Size: 20 preps
This BigDye® Terminator v1.1 sequencing standard consists of four tubes, each of which contains DNA of a known 1,200 base pair sequence prepared with BigDye® Terminator v1.1. Sufficient DNA is included to perform one spectral calibration⁄performance check and⁄or one control sequencing run on an Applied Biosystems® 3500 Dx Series genetic analyzer. The DNA preparation has been lyophilized to maximize stability.
Catalog: 4462113
Size: 4 tubes
Size: 4 tubes
The DS-33 GeneScan™ Installation Standard consists of pooled PCR products labeled with 6-FAM™, VIC®, NED™ and PET® dyes. To generate the pooled products, CEPH individual 1347-02 genomic DNA has been amplified with 8 fluorescent PCR primer pairs which amplify selected microsatellite loci (as listed in the package product insert). The kit also contains a tube of GeneScan™ 600 LIZ™ Size Standard 2.0. For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Catalog: 4376911
Size: 1 kit
Size: 1 kit
The Matrix Standard Kit is used to generate the "multicomponent matrix" required when analyzing 6-FAM™, VIC®, NED™, PET®, and LIZ®- labeled DNA fragments on the Applied Biosystems® 31xx, 3500 and 3730 Series Systems. The Data Collection Software for these instruments uses the multicomponent matrix to automatically analyze the five different colored fluorescent dye-labeled samples in a single capillary. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4345833
Size: 8 runs
Size: 8 runs
This kit contains 4 oligonucleotides respectively labeled with 6-FAM™, VIC®, NED™, and ROX™, pre-pooled in a single tube. Suitable for Dye Set D spectral calibration, on ABI PRISM® Analyzers: 3100-Avant™, 3100, and 3730. Supports 4-Dye fragment analysis applications (Linkage Mapping Set v2.5, Mouse Mapping Primers). The DS-31 Matrix Standard Set is used to generate the "multicomponent matrix" required when analyzing labeled DNA fragments on the Applied Biosystems® 3130 and 3100 Series Systems, 3700 DNA Analyzer, and 3730 Series Systems. The Data Collection Software for these instruments uses t...
Catalog: 4345829
Size: 8 runs
Size: 8 runs
The TA Cloning® Kit with pCR™2.1 vector provides a quick, one-step cloning strategy for directly inserting a Taq -amplified PCR product into a plasmid vector. The TA Cloning® Kit uses the pCR™2.1 cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts. Features of the TA Cloning® Kit with pCR™2.1 vector: • Fast & convenient —15-minute, room-temperature ligation • Efficient —blue/white screening and >80% clones with correct insert • Flexible —choice of kanamyc...
Catalog: K2020-40
Size: 40 reactions
Size: 40 reactions
The DS-30 Matrix Standard kit contains 4 oligonucleotides respectively labeled with 6-FAM™, HEX™, NED™, and ROX™, pre-pooled in a single tube. This Matrix Standard Set is used to generate the "multicomponent matrix" required when analyzing fluorescent dye-labeled DNA fragments on the Applied Biosystems® 3100, 3130, and 3730 Series Systems. The Data Collection Software for these instruments uses the multicomponent matrix to automatically analyze the four different colored fluorescent dye-labeled samples in a single capillary. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4345827
Size: 8 runs
Size: 8 runs
The TA Cloning® Kit Dual Promoter with pCR™II vector provides a quick, one-step cloning strategy for directly inserting a Taq -amplified PCR product into a plasmid vector. The T7 and Sp6 promoters of the pCR™II vector allow in vitro transcription of the insert to produce sense or anti-sense products. The TA Cloning Kit Dual Promoter uses the pCR™II cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts. Features of the TA Cloning® Kit Dual Promoter: • Fast &...
Catalog: K2070-40
Size: 40 reactions
Size: 40 reactions
PENTR/D-TOPO CLONING KIT
Catalog: K240020SP
Size: 20 reactions
Size: 20 reactions
PCR8/GW/TOPO
Catalog: K252020SP
Size: 20 reactions
Size: 20 reactions
This kit includes all of the consumables and accessories needed to perform an instrument qualification—install, operational, or/and performance. This kit is specific for the 3500 Dx CS2 instrument and comes with a 50-cm 8-capillary array, POP-6™ polymer, and Big Dye® Terminator v1.1 sequencing standard.
Catalog: 4462042
Size: 1 kit
Size: 1 kit
This kit includes all of the consumables and accessories needed to perform an instrument qualification—install, operational, or/and performance. This kit is specific for the 3500xL Dx CS2 instrument and comes with a 50-cm 24-capillary array, POP-6™ polymer, and Big Dye® Terminator v1.1 sequencing standard.
Catalog: 4462043
Size: 1 kit
Size: 1 kit
The 5500 W REV1 (DNA) SP Kit contains sequencing primers and probes for DNA fragment libraries used in paired-end sequencing runs. The kit is used to prime the F5 tag for ligation sequencing on the 5500 W FlowChip V2 for DNA libraries. Each kit includes one strip tube containing sufficient specific sequencing primers and bridge probes to run 6 lanes.
Catalog: 4475684
Size: 1 kit
Size: 1 kit
The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337458
Size: 25000 reactions
Size: 25000 reactions
The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337457
Size: 5000 reactions
Size: 5000 reactions
The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337456
Size: 1000 reactions
Size: 1000 reactions
This product can only be used to upgrade Sequencing Analysis Software v5.3.1 installed on standalone computers, computers separate to the instrument data collection system. Please talk with your representative to upgrade the data collection system and secondary analysis software running on the data collection computer. Sequencing Analysis Software enables you to basecall, trim, display, edit, and print data from our entire line of capillary DNA sequencing instruments for data analysis and quality control. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4310991
Size: 1 CD
Size: 1 CD
DNA Sequencing Pcr
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