DNA Polymerase 1
Platinum® Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA Polymerase from Thermococcus species KOD with proofreading (3´a5´ exonuclease) activity. Platinum® antibody technology provides a simple, automatic hot-start method that improves PCR specificity (2,3) (Figure 1). PCR x Enhancer Solution is included for higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved thermostability of Platinum® Pfx DNA Polymera...
Catalog: 11708-013
Size: 100 reactions
Size: 100 reactions
Platinum® Taq DNA Polymerase High Fidelity is ideal for amplification of DNA fragments where high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´a5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase (Table 1). PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology. With Platinum® Taq High Fidelity you'll get: Greater than six times higher fidelity than Taq DNA poly-merase Amplification of fragments up to 15 kb (Figure 1) Room temperature reaction...
Catalog: 11304-102
Size: 5000 reactions
Size: 5000 reactions
Tfi DNA Polymerase is a new non- Taq based, PCR enzyme derived from Thermus filiformis for use in PCR applications without any licensing fees. Like Taq , Tfi has 5'→3' exonuclease and 5'→3' polymerase activity. PCR performance is comparable to Taq DNA polymerase in yield, specificity, fidelity, and robustness (Figure 1, A and B). Tfi can be substituted for Taq DNA Polymerase in virtually any application and is ideal for genomic, cDNA, and plasmid targets up to 4 kb in length. In addition, Tfi is free of licensing restrictions that accompany Taq polymerase. Tfi can be considered for use as a co...
Catalog: 30342-052
Size: 5000 reactions
Size: 5000 reactions
AccuPrime™ Taq DNA Polymerase High Fidelity amplifies nucleic acid templates using antibody-mediated hot-start, a blend of Taq DNA Polymerase and proofreading enzyme, and AccuPrime™ accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases. With AccuPrime™ Taq DNA Polymerase High Fidelity you'll get: The highest specificity and yield for the most robust PCR amplification 9-fold higher fidelity than Taq DNA polymerase alone Mimimal optimization steps, even with non-optimized primer sets Efficient amplification of targets over a broad size range up...
Catalog: 12346-094
Size: 1000 reactions
Size: 1000 reactions
Applied Biosystems® AmpliTaq® DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus ( Taq ) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot. AmpliTaq® DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and efficacy Its thermal activity profile makes it good ...
Catalog: N8080160
Size: 250 units
Size: 250 units
Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 ml of 50 mM MgCl 2 . It is included with Platinum® Taq, Taq, and the SuperScript® First-Strand Synthesis System for RT-PCR.
Catalog: 18067-017
Size: 2 × 1 ml
Size: 2 × 1 ml
1 Kb Plus DNA ladder is a powerful tool for estimating the molecular weight of linear, double stranded DNA fragments. 1 Kb Plus DNA ladder is composed of 20 highly purified, double stranded DNA bands spanning 100 base pairs to 12,000 base pairs. 1 Kb Plus DNA ladder has 12 evenly spaced bands ranging from 1 Kb to 12 Kb, a quick orientation band at 1650 base pairs that forms a distinct doublet with the 2 Kb band, and seven bands of round sizes below 1 Kb. 1 Kb Plus DNA ladder is compatible with agarose gels you cast in your lab as well as E-Gel® pre-cast agarose gels. Round, easy to remember ba...
Catalog: 10787-026
Size: 1000 µg
Size: 1000 µg
1 Kb Plus DNA ladder is a powerful tool for estimating the molecular weight of linear, double stranded DNA fragments. 1 Kb Plus DNA ladder is composed of 20 highly purified, double stranded DNA bands spanning 100 base pairs to 12,000 base pairs. 1 Kb Plus DNA ladder has 12 evenly spaced bands ranging from 1 Kb to 12 Kb, a quick orientation band at 1650 base pairs that forms a distinct doublet with the 2 Kb band, and seven bands of round sizes below 1 Kb. 1 Kb Plus DNA ladder is compatible with agarose gels you cast in your lab as well as E-Gel® pre-cast agarose gels. Round, easy to remember ba...
Catalog: 10787-018
Size: 250 µg
Size: 250 µg
The 1 Kb DNA Extension Ladder is suitable for sizing linear double-stranded DNA fragments from 500 bp to 40 kb. The ladder consists of 8 fragments ranging in length from 1,018 to 8,144 bp in 1,018-bp increments, as well as bands of 506 bp, 517 bp, 1,636 bp, 10 kb, 15 kb, 20 kb, and 40 kb. For easy reference on agarose gels, the 5 kb band is two to three-times brighter than the other bands in the ladder. This ladder can be stained with ethidium bromide or radiolabeled using T4 polynucleotide kinase, T4 DNA polymerase, DNA polymerase I, or the large fragment DNA polymerase I (Klenow fragment).
Catalog: 10511-012
Size: 100 µg
Size: 100 µg
T7 RNA Polymerase is a DNA-dependent RNA polymerase that has a high specificity for bacteriophage T7 promoter sequences. The enzyme synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available. Application: Synthesis of labeled and unlabeled RNA transcripts (1). Source: Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid. Performance and Quality Testing: 3´ and 5´ exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a transcription reaction. Unit De...
Catalog: 18033-100
Size: 2 × 2,500 units
Size: 2 × 2,500 units
E. coli DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of β-NAD between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate cohesive termini. Single-stranded nucleic acids are not substrates for this enzyme. Applications: Second-strand cDNA synthesis (1). T4 DNA Ligase alternative when blunt-end ligation is not required (2). Source: Purified from E. coli 594 (Su - ) bearing λ lysogen gt4lop-11 lig + S7 (3). Performance and Quality Testing: Endodeoxyribonuclease and 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency tested. Unit Definition: One unit is the...
Catalog: 18052-019
Size: 100 units
Size: 100 units
Ambion® TURBO DNA- free ™ contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. The kit includes sufficient reagents for 50 rxns. Features of Ambion® TURBO DNA- free ™ include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can plague RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The Best Method for Genomic DNA Removal Prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNa...
Catalog: AM1907
Size: 50 reactions
Size: 50 reactions
The TrackIt™ format combines the most popular DNA Ladders with loading buffer in a single tube. This format minimizes time spent thawing, diluting, and adding sample buffer to your DNA ladders. Simply transfer your TrackIt™ DNA Ladder from the vial to the gel. These ladders are stable at room temperature and can be stored on your bench top. TrackIt™ DNA Ladders offer two tracking dyes, one that runs behind the sample and one that runs ahead of the sample. The tracking dyes serve as visual markers for following migration during electrophoresis and indicate when maximum resolution of the DNA fra...
Catalog: 10488-022
Size: 20 applications
Size: 20 applications
DNA End Repair Mix offers an easy, one step process for creating blunt ends in sheared, sonicated, or restriction-cut DNA and PCR fragments for efficient ligation into blunt end cloning vectors. DNA End Repair Mix allows you to: Generate blunt ends in DNA and PCR fragments (A-tail) Repair DNA with 5′ or 3′ overhangs in 30 minutes at 37°C Heat inactivate DNA End Repair Mix in 10 minutes at 75°C A Simple Way to Make Blunt Ends With DNA End Repair Mix, DNA with 5′ or 3′ overhangs is converted to 5′ phosphorylated blunt end DNA in 30 minutes at 37°C. Once DNA has been repaired, the enzymes in DNA ...
Catalog: A14321
Size: 20 reactions
Size: 20 reactions
MagMAX™ DNA Multi-Sample 96-Well Kits are designed for rapid purification of high-quality genomic DNA from a variety of sample types including blood and blood cards, tissue, mouse tails, buccal swabs, buffy coats, and cultured cells. These purification kits use MagMAX™ magnetic bead-based nucleic acid isolation technology to produce high yields of purified DNA, free from inhibitors that may affect downstream reactions such as genotyping assays, CE and next-generation sequencing, and high resolution melting analysis. The 96-prep and 5x 96-prep kits are designed for higher throughput and automat...
Catalog: 4413022
Size: 5 x 96 preps
Size: 5 x 96 preps
Ambion® RNase Cocktail™ is a mixture of two highly purified ribonucleases, RNase A (500 U/mL) and RNase T1 (20,000 U/mL), and is free of DNase and nicking activities. Use RNase Cocktail™ for all situations where it is desirable to degrade RNA, i.e. plasmid minipreps and ribonuclease protection assays. Supplied in five tubes containing 1 mL each. Digestion of RNA with RNase A alone leaves fragments of RNA that are large enough to be visible on agarose gels and precipitate in ethanol. RNase A cuts after C and U residues, and RNase T1 cuts after G residues. Consequently, the mixture of both enzym...
Catalog: AM2288
Size: 5 tubes
Size: 5 tubes
Ambion® Lambda DNA is digested to completion with Hind III. This generates fragments of the sizes 23130, 9416, 6557, 4361, 2322, 2027, 564, and 125 bp. These DNA molecular mass markers are supplied with a protocol describing easy labeling of the markers with radioisotopes. They can also be stained with ethidium bromide. One tube containing 200 μg is provided.
Catalog: AM7720
Size: 200 µg
Size: 200 µg
The PureLink® Viral RNA/DNA Mini Kit is a nucleic acid purification system designed for fast and easy isolation of viral RNA or DNA from cell-free samples, such as serum, plasma, and cerebrospinal fluid. Starting sample volumes can be as high as 500 µl and elution volumes as low as 10 µl, allowing for a 50-fold concentration of nucleic acids. The higher target concentration obtained using the PureLink® Viral RNA/ DNA Mini Kit provides greater sensitivity and accuracy in downstream detection procedures such as real-time PCR or endpoint analysis. Specially formulated buffers enable the purificat...
Catalog: 12280-050
Size: 50 preps
Size: 50 preps
Ambion® TURBO DNA- free ™ contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. The kit includes sufficient reagents for 50 rxns. Features of Ambion® TURBO DNA- free ™ include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can plague RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The Best Method for Genomic DNA Removal Prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNa...
Catalog: AM1907M
Size: 50 rxns w⁄ Manual
Size: 50 rxns w⁄ Manual
DNA Polymerase 1
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