DNA Methylation Analysis
The BRAF Mutation Analysis Reagents employ a simple fragment analysis workflow to detect 1% mutant to wild type genomic DNA across 3 common and rare mutations in the BRAF gene region (see Table 1). The BRAF set provides sufficient reagent for analysis of 30 samples. Advantages of the BRAF Mutation Analysis Reagents include: • Simple Analysis—unambiguous, easy-to-interpret results • Thorough Coverage—detects 3 mutations in the BRAF gene • Sensitive—detects mutations at frequencies as low as 1 to 5% against a wild type genomic DNA background • Efficient—interrogate multiple loci from the same sa...
Catalog: 4452136
Size: 1 kit
Size: 1 kit
This product can only be used to upgrade Sequencing Analysis Software v5.3.1 installed on standalone computers, computers separate to the instrument data collection system. Please talk with your representative to upgrade the data collection system and secondary analysis software running on the data collection computer. Sequencing Analysis Software enables you to basecall, trim, display, edit, and print data from our entire line of capillary DNA sequencing instruments for data analysis and quality control. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4310991
Size: 1 CD
Size: 1 CD
E. coli DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of β-NAD between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate cohesive termini. Single-stranded nucleic acids are not substrates for this enzyme. Applications: Second-strand cDNA synthesis (1). T4 DNA Ligase alternative when blunt-end ligation is not required (2). Source: Purified from E. coli 594 (Su - ) bearing λ lysogen gt4lop-11 lig + S7 (3). Performance and Quality Testing: Endodeoxyribonuclease and 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency tested. Unit Definition: One unit is the...
Catalog: 18052-019
Size: 100 units
Size: 100 units
The TURBO DNA- free ™ Kit contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. Features of the Ambion® TURBO DNA- free ™ Kit include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can interfere with RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The best method for genomic DNA removal prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNase I that is much more efficient t...
Catalog: AM1907
Size: 50 reactions
Size: 50 reactions
The TrackIt™ format combines the most popular DNA Ladders with loading buffer in a single tube. This format minimizes time spent thawing, diluting, and adding sample buffer to your DNA ladders. Simply transfer your TrackIt™ DNA Ladder from the vial to the gel. These ladders are stable at room temperature and can be stored on your bench top. TrackIt™ DNA Ladders offer two tracking dyes, one that runs behind the sample and one that runs ahead of the sample. The tracking dyes serve as visual markers for following migration during electrophoresis and indicate when maximum resolution of the DNA fra...
Catalog: 10488-022
Size: 20 applications
Size: 20 applications
FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor® colors or get the FISH Tag™ DNA Multicolor Kit , which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. FISH Tag™ Kit Specificatio...
Catalog: F32947
Size: 1 kit
Size: 1 kit
1 Kb Plus DNA ladder is a powerful tool for estimating the molecular weight of linear, double stranded DNA fragments. 1 Kb Plus DNA ladder is composed of 20 highly purified, double stranded DNA bands spanning 100 base pairs to 12,000 base pairs. 1 Kb Plus DNA ladder has 12 evenly spaced bands ranging from 1 Kb to 12 Kb, a quick orientation band at 1650 base pairs that forms a distinct doublet with the 2 Kb band, and seven bands of round sizes below 1 Kb. 1 Kb Plus DNA ladder is compatible with agarose gels you cast in your lab as well as E-Gel® pre-cast agarose gels. Round, easy to remember ba...
Catalog: 10787-026
Size: 1000 µg
Size: 1000 µg
The FISH Tag™ DNA Multicolor Kit provides a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. The FISH Tag™ DNA Multicolor Kit contains fluorescent dyes in four colors, making it ideal for simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. We also offer FISH Tag™ Kits in single colors. FISH Tag™ Kit Specificati...
Catalog: F32951
Size: 1 kit
Size: 1 kit
DNA End Repair Mix offers an easy, one step process for creating blunt ends in sheared, sonicated, or restriction-cut DNA and PCR fragments for efficient ligation into blunt end cloning vectors. DNA End Repair Mix allows you to: Generate blunt ends in DNA and PCR fragments (A-tail) Repair DNA with 5′ or 3′ overhangs in 30 minutes at 37°C Heat inactivate DNA End Repair Mix in 10 minutes at 75°C A Simple Way to Make Blunt Ends With DNA End Repair Mix, DNA with 5′ or 3′ overhangs is converted to 5′ phosphorylated blunt end DNA in 30 minutes at 37°C. Once DNA has been repaired, the enzymes in DNA ...
Catalog: A14321
Size: 20 reactions
Size: 20 reactions
The ApoTarget™ Quick Apoptotic DNA Ladder Detection Kit provides a simple and rapid procedure for extraction of chromosomal DNA. The procedure prepares DNA for use in the methods that detect DNA fragmentation in apoptotic cells. Unlike other kits requiring 1 to 2 days to finish, this detection method requires only less than 90 minutes to prepare DNA in a single tube without the need for extraction or column steps. DNA fragmentation can be easily visualized by agarose gel electrophoresis. This procedure increases recovery of small fragmented DNA, thereby improving the sensitivity of the assay.
Catalog: KHO1021
Size: 50 tests
Size: 50 tests
MagMAX™ DNA Multi-Sample 96-Well Kits are designed for rapid purification of high-quality genomic DNA from a variety of sample types including blood and blood cards, tissue, mouse tails, buccal swabs, buffy coats, and cultured cells. These purification kits use MagMAX™ magnetic bead-based nucleic acid isolation technology to produce high yields of purified DNA, free from inhibitors that may affect downstream reactions such as genotyping assays, CE and next-generation sequencing, and high resolution melting analysis. The 96-prep and 5x 96-prep kits are designed for higher throughput and automat...
Catalog: 4413022
Size: 5 x 96 preps
Size: 5 x 96 preps
1 Kb Plus DNA ladder is a powerful tool for estimating the molecular weight of linear, double stranded DNA fragments. 1 Kb Plus DNA ladder is composed of 20 highly purified, double stranded DNA bands spanning 100 base pairs to 12,000 base pairs. 1 Kb Plus DNA ladder has 12 evenly spaced bands ranging from 1 Kb to 12 Kb, a quick orientation band at 1650 base pairs that forms a distinct doublet with the 2 Kb band, and seven bands of round sizes below 1 Kb. 1 Kb Plus DNA ladder is compatible with agarose gels you cast in your lab as well as E-Gel® pre-cast agarose gels. Round, easy to remember ba...
Catalog: 10787-018
Size: 250 µg
Size: 250 µg
The 1 Kb DNA Extension Ladder is suitable for sizing linear double-stranded DNA fragments from 500 bp to 40 kb. The ladder consists of 8 fragments ranging in length from 1,018 to 8,144 bp in 1,018-bp increments, as well as bands of 506 bp, 517 bp, 1,636 bp, 10 kb, 15 kb, 20 kb, and 40 kb. For easy reference on agarose gels, the 5 kb band is two to three-times brighter than the other bands in the ladder. This ladder can be stained with ethidium bromide or radiolabeled using T4 polynucleotide kinase, T4 DNA polymerase, DNA polymerase I, or the large fragment DNA polymerase I (Klenow fragment).
Catalog: 10511-012
Size: 100 µg
Size: 100 µg
Ambion® Lambda DNA is digested to completion with Hind III. This generates fragments of the sizes 23130, 9416, 6557, 4361, 2322, 2027, 564, and 125 bp. These DNA molecular mass markers are supplied with a protocol describing easy labeling of the markers with radioisotopes. They can also be stained with ethidium bromide. One tube containing 200 μg is provided.
Catalog: AM7720
Size: 200 µg
Size: 200 µg
The PureLink® Viral RNA/DNA Mini Kit is a nucleic acid purification system designed for fast and easy isolation of viral RNA or DNA from cell-free samples, such as serum, plasma, and cerebrospinal fluid. Starting sample volumes can be as high as 500 µl and elution volumes as low as 10 µl, allowing for a 50-fold concentration of nucleic acids. The higher target concentration obtained using the PureLink® Viral RNA/ DNA Mini Kit provides greater sensitivity and accuracy in downstream detection procedures such as real-time PCR or endpoint analysis. Specially formulated buffers enable the purificat...
Catalog: 12280-050
Size: 50 preps
Size: 50 preps
The TURBO DNA- free ™ Kit contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. Features of the Ambion® TURBO DNA- free ™ Kit include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can interfere with RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The best method for genomic DNA removal prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNase I that is much more efficient t...
Catalog: AM1907M
Size: 50 rxns w⁄ Manual
Size: 50 rxns w⁄ Manual
This real-time PCR-based kit is designed for the quantification of amplifiable human DNA that is present in a sample, and has been validated for human identification applications. An internal PCR control is formulated into each reaction to identify samples that may contain PCR inhibitors. • Quantifies DNA from a wide variety of sources including buccal swabs, blood, semen, tissue, and other human DNA sources making this kit suitable for casework, databasing, and paternity labs. • Chemistry compatible with commonly used extraction technologies for easy integration into current lab workflow. • P...
Catalog: 4343895
Size: 400 reactions
Size: 400 reactions
The resDNASEQ® MDCK Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from MDCK (canine kidney) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of MDCK cell DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high molecular weight or sheared DNA. T...
Catalog: 4464335
Size: 100 reactions
Size: 100 reactions
The resDNASEQ® MDCK Residual DNA Quantitation System is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from MDCK (canine kidney) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of MDCK cell DNA, typically in less than 4 hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high...
Catalog: 4464337
Size: 100 reactions
Size: 100 reactions
The resDNASEQ® NS0 Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from NS0 (murine myeloma) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of NS0 cell DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high molecular weight or sheared DNA. Thi...
Catalog: 4458441
Size: 100 reactions
Size: 100 reactions
DNA Methylation Analysis
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