DNA Clone
The TA Cloning® Kit with pCR™2.1 vector provides a quick, one-step cloning strategy for directly inserting a Taq -amplified PCR product into a plasmid vector. The TA Cloning® Kit uses the pCR™2.1 cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts. Features of the TA Cloning® Kit with pCR™2.1 vector: • Fast & convenient —15-minute, room-temperature ligation • Efficient —blue/white screening and >80% clones with correct insert • Flexible —choice of kanamyc...
Catalog: K2020-40
Size: 40 reactions
Size: 40 reactions
Zero Blunt® TOPO® PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR®-Blunt II-TOPO® Vector for 5-minute benchtop ligations without ligase (1). The pCR®-Blunt II-TOPO® Vector also features: • ccdB gene for positive selection (2) • Eco R I sites flanking the PCR product insertion site for easy excision of inserts • Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli • SP6 promoter/primer site for...
Catalog: K2800-02
Size: 20 preps
Size: 20 preps
The GeneArt® Linear pUC19L Vector is a ready-to-use, linear version of the popular cloning vector pUC19. The pUC19L plasmid (2.7 Kbp), is specifically designed as a cloning and DNA assembly vector for the GeneArt® Seamless Cloning and Assembly Kit (cat # A13288). The vector is manufactured by digesting supercoiled pUC19 plasmid with the restriction enzymes PstI and KpnI, and tested for cloning of up to 4 DNA fragments. Some of the key features and elements of the pUC19L vector include: • lacZα gene allows easy blue⁄white colony screening for selection of recombinants • pUC origin of replicatio...
Catalog: A13289
Size: 20 reactions
Size: 20 reactions
The TOPO® TA Cloning® Kits for Sequencing provide a highly efficient, 5 minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing. Each kit uses the pCR™4-TOPO® TA vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction. These kits include everything necessary to clone and select recombinant vectors containing your PCR fragment of choice. Get More Sequence— Allows for more insert sequence and less vector seuquence when usin...
Catalog: K4530-20
Size: 20 reactions
Size: 20 reactions
PENTR/D-TOPO CLONING KIT
Catalog: K240020SP
Size: 20 reactions
Size: 20 reactions
The TA Cloning® Kit Dual Promoter with pCR™II vector provides a quick, one-step cloning strategy for directly inserting a Taq -amplified PCR product into a plasmid vector. The T7 and Sp6 promoters of the pCR™II vector allow in vitro transcription of the insert to produce sense or anti-sense products. The TA Cloning Kit Dual Promoter uses the pCR™II cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts. Features of the TA Cloning® Kit Dual Promoter: • Fast &...
Catalog: K2070-40
Size: 40 reactions
Size: 40 reactions
The pLenti6⁄V5 Directional TOPO® Cloning Kit contains the TOPO®-adapted ViraPower™ lentiviral expression vector, pLenti6⁄V5-D-TOPO® for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving high-level, constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells. Advantages • High efficiency and rapid cloning • Constitutive gene expression in dividing and non-dividing mammalian cells in vitro or in vivo • Produces replication-incompet...
Catalog: K4955-10
Size: 20 reactions
Size: 20 reactions
Invitrogen's™ pLenti6.3⁄V5™-TOPO®Cloning Kit is part of our ViraPower™ HiPerform™ Lentiviral Expression System (catalog K5310-00). The pLenti6.3⁄V5™-TOPO® -TA Cloning Kit contains the TOPO®- adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5™-TOPO® for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5™-TOPO® vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequenc...
Catalog: K5315-20
Size: 1 kit
Size: 1 kit
The pENTR™/SD/D-TOPO® Cloning Kits utilize a highly efficient, 5-minute cloning strategy ("TOPO® Cloning") to directionally clone a blunt-end PCR product into a vector for entry into the Gateway® System or the MultiSite Gateway® System. Blunt-end PCR products clone directionally into the pENTR™/SD/D-TOPO® entry vector at greater than 90% efficiency, and include an upstream Shine-Dalgarno sequence for expression in prokaryotes. The kit comes with everything necessary to clone and select your PCR amplified gene of interest. Gateway® System Ready - Rapidly shuttle cloned genes between multiple ve...
Catalog: K2420-20
Size: 20 reactions
Size: 20 reactions
The pCR®8/GW/TOPO® TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway® recombination cloning vector platform. Our pCR®8 TOPO® TA vector contains a minimal MCS, so you sequence more of your insert and less of the vector. With our patented att sites, the pCR®8 vector is the simplest way to create an Entry Clone, which facilitates easy access via Gateway® recombination cloning to a variety of expression vectors without tedious sub-cloning, saving you valuable research time. The pCR®8/GW/TOPO® TA Cloning Kit offers: att sites for rapid recombination into a variety of Gateway...
Catalog: K250020
Size: 20 reactions
Size: 20 reactions
The pcDNA™3.3-TOPO® vector is designed to deliver exceptionally high levels of transgene expression in adherent cells as well as in the FreeStyle™ MAX CHO and FreeStyle™ MAX 293 suspension cell systems. The pcDNA3.3™-TOPO® TA Cloning® Kit is supplied with the TOPO®-adapted plasmid vector, all reagents for cloning, and One Shot® TOP10 competent cells. The pcDNA™3.3-TOPO® vector offers the following benefits: • TOPO® adapted for cloning PCR products with >85% efficiency • High-level gene expression in a wide range of mammalian cells due to the native cytomegalovirus (CMV) immediate-early promote...
Catalog: K8300-01
Size: 20 reactions
Size: 20 reactions
GeneArt® Cloning into pcDNA™3.1 (+)
Catalog: 854341DE
Size: 1 each
Size: 1 each
GeneArt® Service Cloning pFastBac1 (Ready-to-Work Package)
Catalog: 855324DE
Size: 1 each
Size: 1 each
YEAST HOMOZYGOUS DIPLOID PLATE
Catalog: FL1002
Size: each
Size: each
PCRII TOPO TA Cloning Kit
Catalog: 10351021
Size: 20 reactions
Size: 20 reactions
RPCI-11 HS BAC CLONE
Catalog: RPCI11.C
PCR8/GW/TOPO
Catalog: K252020SP
Size: 20 reactions
Size: 20 reactions
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA6.2/V5-DEST vector offers the following key features: •Cytomegalovirus (CMV) promoter for high-level expression • att R sites for Gateway® cloning, enabling recombination with att L-flanked fragments •C-terminal V5 tag for easy detection •Blasticidin resistance gene for efficient stable selection •Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli Gateway® Cloning To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® d...
Catalog: 12489-027
Size: 6 µg
Size: 6 µg
The PichiaPink™ Secretion Signal Set is a set of 8 DNA fragments encoding secretion signal sequences. The PichiaPink™ Secretion Signal Set is designed to be easily ligated in front of your gene. The PichiaPink™ Secretion Signal Set is designed for use with the pPINK-HC and pPINK-LC vectors. Optimize Secretion of Your Expressed Protein By adding one of the 8 secretion signal sequences to your gene, you can secrete your protein into the media to make it easier to harvest and purify. Pichia pastoris secretes few native proteins, so the bulk of the protein in the media will be yours. Each DNA sequ...
Catalog: A11155
Size: 8 each
Size: 8 each
DNA Clone
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