DNA Array
E. coli DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of β-NAD between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate cohesive termini. Single-stranded nucleic acids are not substrates for this enzyme. Applications: Second-strand cDNA synthesis (1). T4 DNA Ligase alternative when blunt-end ligation is not required (2). Source: Purified from E. coli 594 (Su - ) bearing λ lysogen gt4lop-11 lig + S7 (3). Performance and Quality Testing: Endodeoxyribonuclease and 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency tested. Unit Definition: One unit is the...
Catalog: 18052-019
Size: 100 units
Size: 100 units
The TURBO DNA- free ™ Kit contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. Features of the Ambion® TURBO DNA- free ™ Kit include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can interfere with RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The best method for genomic DNA removal prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNase I that is much more efficient t...
Catalog: AM1907
Size: 50 reactions
Size: 50 reactions
The TaqMan® Array Human MicroRNA B Card v3.0 contains 384 TaqMan® MicroRNA Assays enabling accurate quantitation of 377 human microRNAs in as little as 5 hours. Three TaqMan MicroRNA Assay endogenous controls are included to aid in data normalization and one TaqMan® MicroRNA Assay not related to human is included as a negative control. Details on the included assays is available in the Target List file. Purchase of the Megaplex™ RT Primers, Human Pool B v3.0 is required to run this array. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Human Pool B v3.0 for situatio...
Catalog: 4444910
Size: 4 pack
Size: 4 pack
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human p53 Signaling 96-well Plate contains 92 assays to p53 signaling associated genes and 4 assays to candidate endogenous control genes.
Catalog: 4418813
Size: 1 Fast plate
Size: 1 Fast plate
The Applied Biosystems® TaqMan® Array Human Androgens 96-well Plate contains 92 assays to androgen associated genes and 4 assays to candidate endogenous control genes. The panel of assays in the TaqMan® Array Human Androgens 96-well Plate targets genes which code for enzymes involved in androgen synthesis, regulators of androgen production, and receptors and signal transduction proteins which mediate their effects, and targets of androgen signaling. The panel also includes the Y-chromosome SRY genes, which play roles in male phenotype development, as well as differentiation of the bipotential ...
Catalog: 4414083
Size: 1 standard plate
Size: 1 standard plate
The Applied Biosystems® TaqMan® Array Human Transcription of tRNA 96-well Plate contains 28 assays to transcription of tRNA associated genes and 4 assays to candidate endogenous control genes. All assays are plated in triplicate. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. • Affordable; m...
Catalog: 4414196
Size: 1 standard plate
Size: 1 standard plate
The TrackIt™ format combines the most popular DNA Ladders with loading buffer in a single tube. This format minimizes time spent thawing, diluting, and adding sample buffer to your DNA ladders. Simply transfer your TrackIt™ DNA Ladder from the vial to the gel. These ladders are stable at room temperature and can be stored on your bench top. TrackIt™ DNA Ladders offer two tracking dyes, one that runs behind the sample and one that runs ahead of the sample. The tracking dyes serve as visual markers for following migration during electrophoresis and indicate when maximum resolution of the DNA fra...
Catalog: 10488-022
Size: 20 applications
Size: 20 applications
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Extracellular Matrix & Adhesion Molecules 96-well Plate contains 92 assays to extracellular matrix & adhesion molecules associated genes and 4 assays to candidate endogenous control genes.
Catalog: 4418778
Size: 1 Fast plate
Size: 1 Fast plate
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Transcription of mRNA 96-well Plate contains 44 assays to transcription of mRNA associated genes and 4 assays to candidate endogenous control genes. All assays are plated in duplicate.
Catalog: 4418839
Size: 1 Fast plate
Size: 1 Fast plate
The Applied Biosystems® TaqMan® Array Human Cardiomyocyte Differentiation by BMP Receptors 96-well Plate contains 28 assays to Cardiomyocyte Differentiation by BMP Receptors associated genes and 4 assays to candidate endogenous control genes. All assays are plated in triplicate. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene s...
Catalog: 4414115
Size: 1 standard plate
Size: 1 standard plate
1 Kb Plus DNA ladder is a powerful tool for estimating the molecular weight of linear, double stranded DNA fragments. 1 Kb Plus DNA ladder is composed of 20 highly purified, double stranded DNA bands spanning 100 base pairs to 12,000 base pairs. 1 Kb Plus DNA ladder has 12 evenly spaced bands ranging from 1 Kb to 12 Kb, a quick orientation band at 1650 base pairs that forms a distinct doublet with the 2 Kb band, and seven bands of round sizes below 1 Kb. 1 Kb Plus DNA ladder is compatible with agarose gels you cast in your lab as well as E-Gel® pre-cast agarose gels. Round, easy to remember ba...
Catalog: 10787-026
Size: 1000 µg
Size: 1000 µg
DNA End Repair Mix offers an easy, one step process for creating blunt ends in sheared, sonicated, or restriction-cut DNA and PCR fragments for efficient ligation into blunt end cloning vectors. DNA End Repair Mix allows you to: Generate blunt ends in DNA and PCR fragments (A-tail) Repair DNA with 5′ or 3′ overhangs in 30 minutes at 37°C Heat inactivate DNA End Repair Mix in 10 minutes at 75°C A Simple Way to Make Blunt Ends With DNA End Repair Mix, DNA with 5′ or 3′ overhangs is converted to 5′ phosphorylated blunt end DNA in 30 minutes at 37°C. Once DNA has been repaired, the enzymes in DNA ...
Catalog: A14321
Size: 20 reactions
Size: 20 reactions
MagMAX™ DNA Multi-Sample 96-Well Kits are designed for rapid purification of high-quality genomic DNA from a variety of sample types including blood and blood cards, tissue, mouse tails, buccal swabs, buffy coats, and cultured cells. These purification kits use MagMAX™ magnetic bead-based nucleic acid isolation technology to produce high yields of purified DNA, free from inhibitors that may affect downstream reactions such as genotyping assays, CE and next-generation sequencing, and high resolution melting analysis. The 96-prep and 5x 96-prep kits are designed for higher throughput and automat...
Catalog: 4413022
Size: 5 x 96 preps
Size: 5 x 96 preps
The Applied Biosystems® TaqMan® Mouse GPCR Array is an efficient, easy-to-use micro fluidic card for quantitative gene expression analysis of mouse GPCR genes important in drug discovery. This product is designed with 380 TaqMan® Gene Expression Assays arrayed in singletons in 384 wells (plus 1 control arrayed in quadruplicate) and is compatible with an upgraded Applied Biosystems 7900HT Fast Real-Time PCR System. The targets include retinal receptors, small molecule receptors, chemokine receptors and others including classic endogenous control targets. This product is packaged in 1 box contai...
Catalog: 4378703
Size: 4 cards
Size: 4 cards
The TaqMan® Human Immune Array from Applied Biosystems® is an efficient, easy-to-use micro fluidic card for quantitative gene expression analysis of targets known to have implications in immune response. This product is designed with 96 TaqMan® Gene Expression Assays loaded in singletons into the array’s 384 wells and is compatible with an upgraded Applied Biosystems 7900HT Fast Real-Time PCR System. The targets include cytokines, chemokines, growth factors, immune regulators, apoptosis markers, ischemia markers, tissue-specific markers, and others including classic endogenous markers.This pro...
Catalog: 4370573
Size: 4 cards
Size: 4 cards
The Applied Biosystems® TaqMan® Array Mouse Immune Response 96-well Plate contains 92 assays to immune response associated genes and 4 assays to candidate endogenous control genes. The panel of assays in this plate targets genes from immune system functions that fall into 9 classes: Cell Surface Receptors; Stress Response; Oxidoreductases; Proteases; Transcription Factors; Signal Transduction; Cytokines and Cytokine Receptors; Chemokines and Chemokine Receptors; and Cell Cycle and Protein Kinases. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan...
Catalog: 4414079
Size: 1 standard plate
Size: 1 standard plate
1 Kb Plus DNA ladder is a powerful tool for estimating the molecular weight of linear, double stranded DNA fragments. 1 Kb Plus DNA ladder is composed of 20 highly purified, double stranded DNA bands spanning 100 base pairs to 12,000 base pairs. 1 Kb Plus DNA ladder has 12 evenly spaced bands ranging from 1 Kb to 12 Kb, a quick orientation band at 1650 base pairs that forms a distinct doublet with the 2 Kb band, and seven bands of round sizes below 1 Kb. 1 Kb Plus DNA ladder is compatible with agarose gels you cast in your lab as well as E-Gel® pre-cast agarose gels. Round, easy to remember ba...
Catalog: 10787-018
Size: 250 µg
Size: 250 µg
The 1 Kb DNA Extension Ladder is suitable for sizing linear double-stranded DNA fragments from 500 bp to 40 kb. The ladder consists of 8 fragments ranging in length from 1,018 to 8,144 bp in 1,018-bp increments, as well as bands of 506 bp, 517 bp, 1,636 bp, 10 kb, 15 kb, 20 kb, and 40 kb. For easy reference on agarose gels, the 5 kb band is two to three-times brighter than the other bands in the ladder. This ladder can be stained with ethidium bromide or radiolabeled using T4 polynucleotide kinase, T4 DNA polymerase, DNA polymerase I, or the large fragment DNA polymerase I (Klenow fragment).
Catalog: 10511-012
Size: 100 µg
Size: 100 µg
Ambion® Lambda DNA is digested to completion with Hind III. This generates fragments of the sizes 23130, 9416, 6557, 4361, 2322, 2027, 564, and 125 bp. These DNA molecular mass markers are supplied with a protocol describing easy labeling of the markers with radioisotopes. They can also be stained with ethidium bromide. One tube containing 200 μg is provided.
Catalog: AM7720
Size: 200 µg
Size: 200 µg
The PureLink® Viral RNA/DNA Mini Kit is a nucleic acid purification system designed for fast and easy isolation of viral RNA or DNA from cell-free samples, such as serum, plasma, and cerebrospinal fluid. Starting sample volumes can be as high as 500 µl and elution volumes as low as 10 µl, allowing for a 50-fold concentration of nucleic acids. The higher target concentration obtained using the PureLink® Viral RNA/ DNA Mini Kit provides greater sensitivity and accuracy in downstream detection procedures such as real-time PCR or endpoint analysis. Specially formulated buffers enable the purificat...
Catalog: 12280-050
Size: 50 preps
Size: 50 preps
DNA Array
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