Dextran Fluorescein Conjugation Protocol
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
Catalog: A-2662
Size: 1 mg
Size: 1 mg
Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection. Dextran Specifications : Label (Ex/Em): Rhodamine B (570/590) Size: 70,000 MW Charge: Zwitterionic Fixable: Nonfixable High Manufacturing Standards of Molecular Probes® Dextrans We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Dextrans are hydrop...
Catalog: D-1841
Size: 25 mg
Size: 25 mg
CD32, Mouse Anti-Human, (FITC); FITC conjugated Monoclonal antibody specific to Human CD32. This antibody is validated for use in Flow Cytometry. Anti-CD32 recognizes the expressed product of the FCGR2A gene.
Catalog: CD3201
Size: 0.5 ml
Size: 0.5 ml
The pcDNA™6 BioEase™ Gateway® Biotinylation System provides an easy, efficient method for expressing, purifying, and detecting biotinylated recombinant proteins. The pcDNA™6/BioEase™-DEST vector (Figure 1) incorporates a 72 amino acid sequence from K. pneumoniae that directs in vivo biotinylation of a specific lysine residue. This K. pneumoniae tag is recognized and efficiently biotinylated in vivo by a native mammalian system similar to the enzyme Biotin Protein Ligase (BPL) in E. coli (1). Proteins produced from the pcDNA™6/BioEase™-DEST vector are expressed as fusion proteins to this sequen...
Catalog: SA100-02
Size: 50 µl
Size: 50 µl
Molecular Probes® cholera toxin conjugates are made from a recombinant version of the B subunit only. This allows us to provide a very high-purity product that is completely free of the toxic A subunit. Cholera toxin B subunit (CT-B) attaches to cells by binding to ganglioside G M1 , making it a powerful tool for retrograde labeling of neurons. This tracer has been used in a variety of applications, including tracing of rat forebrain afferents, projections of the parabrachial region, and neurons of the urinary bladder wall. When used in neuronal tracing applications, CT-B is typically introduc...
Catalog: C-34778
Size: 100 µg
Size: 100 µg
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
Catalog: F-150
Size: 25 mg
Size: 25 mg
This purified Mouse Histidine (C-term) Tag Monoclonal Antibody recognizes a polyhistidine amino acid sequence at the carboxy-terminus of proteins. The free carboxy-terminus of the terminal histidine residue is a key element of the epitope recognition site of the anti-His (C-term) Antibody. The Anti-His(C-term) Monoclonal Antibody recognizes the sequence: -His-His-His-His-His-His-COOH . Validated applications for Histidine (C-term) Tag monoclonal antibody are western blotting and ELISA. Antibody Specifications: • Applications: Validated applications for Histidine (C-term) Tag Monoclonal Antibod...
Catalog: R931-25
Size: 50 µl
Size: 50 µl
Lectin phytohemagglutinin-L (PHA-L) is strongly inhibited by N-acetylglucosamine β(1-2) mannopyranosyl residues.[ALTREF PN29369] This lectin has been shown to agglutinate leukocytes and induce mitogenic activity in T-lymphocytes[ALTREF PN29369,PN29368] and has also been used as an anterograde tracer for the study of neuronal pathways.[ALTREF PN20284]
Catalog: L32457
Size: 1 mg
Size: 1 mg
The IgG (γ), goat F(ab’), 2 R-phycoerythrin (R-PE) dye conjugate is a polyclonal goat antibody that is Fc specific and recognizes human IgG. R-PE is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence emission without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients. Use of R-PE in antibody conjugates results in probes with greatly enhanced detectability. Antibody Specifications Description: IgG (γ), Goat F(ab’)2 polyclonal, Fc specific Label: R-PE Validated Appl...
Catalog: H10104
Size: 0.5 ml
Size: 0.5 ml
TCR ALPHA BETA, Hamster Anti-Mouse, (Alexa Fluor 488); Alexa Fluor 488 conjugated Monoclonal antibody specific to Mouse TCR α/β. This antibody is validated for use in Flow Cytometry. Anti-TCR α/β recognizes the expressed product of the TCRA/B gene.
Catalog: HM3620
Size: 1 ml
Size: 1 ml
Our horseradish peroxidase (HRP) conjugate of NeutrAvidin® biotin-binding protein - a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point - can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding.
Catalog: A-2664
Size: 1 mg
Size: 1 mg
Our streptavidin, Marina Blue™ conjugate is a bright, blue-fluorescent probe with excitation/emission ~365/460 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.
Catalog: S-11221
Size: 1 mg
Size: 1 mg
Affinity purified F(ab')2 gragment of goat anti-rat IgG conjugated to horseradish peroxidase. To minimize cross-reactivity, this antibody has been absorbed against human IgG, human serum, mouse IgG, mouse serum and bovine serum.
Catalog: A10548
Size: 0.5 mg
Size: 0.5 mg
Lectin PNA is specific for terminal β-galactose. It will agglutinate human erythrocytes, but only after neuraminidase treatment.[ALTREF PN40265]
Catalog: L32458
Size: 1 mg
Size: 1 mg
Clone/PAD: PY20 Immunogen: Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine Specificity: Reacts specifically with tyrosine phosphorylated proteins in the native or denatured states, and does not react with phosphoserine or phosphothreonine. Reacts with a wide variety of tyrosine-phosphorylated proteins in all tested species. Reactivity: Human, Mouse, (positive controls: mouse brain, NIH 3T3 cells (+/- TPA), K562 cells, EGF-stimulated A431 cells, and MCF-7 cell lysates) Applications: Western blotting, Immunoprecipitation, ELISA, Affinity chromotograp...
Catalog: 03-7722
Size: 100 µg
Size: 100 µg
In collaboration with Nexins Research BV, we provide the best and brightest annexin V conjugates available, including this FITC annexin V conjugate. Highly fluorescent annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, one of the earliest indicators of apoptosis.
Catalog: A13199
Size: 500 µl
Size: 500 µl
Fluorescein di-β-D-galactopyranoside (FDG) is one of the most sensitive substrates for galactosidases. Nonfluorescent FDG is sequentially hydrolyzed by β-galactosidase, first to fluorescein monogalactoside (FMG) and then to highly fluorescent fluorescein. Enzyme-mediated hydorlysis of FDG can be followed by the increase in either absorbance or fluorescence.
Catalog: F-1179
Size: 5 mg
Size: 5 mg
The red-orange-fluorescent BODIPY® 564/570 paclitaxel can be used to study microtubules in solution.
Catalog: P7501
Size: 10 µg
Size: 10 µg
Streptavidin-Horseradish Peroxidase (HRP) conjugate
Catalog: SA100-01
Size: 50 µl
Size: 50 µl
Clone/PAD: Z021 Immunogen: Synthetic biotin Specificity: Binds strongly to biotinylated proteins. Reactivity: Biotinylated proteins Applications: ELISA, Cell and tissue staining
Catalog: 03-3720
Size: 1 ml
Size: 1 ml
Dextran Fluorescein Conjugation Protocol
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