Custom Rna

Custom Plus TaqMan® RNA Assay, MED

Category: Products & Services > Oligos, Primers, Probes & Nucleotides

Custom Plus TaqMan® RNA Assay, MED
Catalog: 4441117
Size: 750 µl

Arcturus® Paradise® Plus RNA Extraction and Isolation

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits

The ARCTURUS® Paradise® PLUS FFPE RNA Isolation Kit permits unprecedented gene expression analysis of millions of previously inaccessible samples by unlocking RNA from formalin-fixed, paraffin-embedded (FFPE) tissues. Amplify as little as 5 ng of the isolated fixed RNA using reagents and methods that have been specially optimized to overcome the challenges of cross-linked FFPE templates (Figure 1). Secure Downstream Success with the Paradise® QC Kit Not all FFPE samples contain high quality RNA. Identify the best samples for your studies using a simple, real-time PCR research technique, and th...
Catalog: KIT0312-I
Size: 12 samples

Illumina® TotalPrep™-96 RNA Amplification Kit

Category: Products & Services > Kits & Assays > RNA Amplification Kits

The Ambion® TotalPrep™ RNA Amplification Kit (patent pending) is a complete system for generating biotinylated, amplified RNA for hybridization with Illumina Sentrix® arrays. The kit includes sufficient reagents for 96 reactions. • Perform amplification from just 50 ng of input RNA • Developed for expression profiling using the Sentrix® BeadChips • Bio-16-UTP now included in optimized NTP mix, reducing setup time and maximizing labeling The TotalPrep™ RNA Amplification Kit incorporates extensive improvements to the in vitro transcription reaction (IVT) over the previous TotalPrep™ Kit. The IVT...
Catalog: 4393543
Size: 96 reactions

PureLink® RNA Mini Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits

The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. • Rapid, Non-Hazardous RNA purification capabilities • Ready-to-use RNA for many downstream applications Rapid and Convenient Column Purification Procedures The PureLink® RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and t...
Catalog: 12183018A
Size: 50 preps

iPrep™ Trizol® Plus RNA Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits

Use the iPrep™ Trizol® Plus RNA Kit for the highest purity, even from difficult samples such as fatty tissue. This kit provides reproducible RNA sample preparation from up to 50 mg of tissue, 10e7 cultured cells, 10 ml blood, and plant samples. The kit contains pre-filled, fully qualified cartridges to avoid any mix-ups or cross-contamination. It also provides low CVs and reduced purification variability, increasing the reliability of your gene expression assays.
Catalog: IS-10007
Size: 1 kit

T7 RNA Polymerase

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > DNA⁄RNA Polymerases

T7 RNA Polymerase is a DNA-dependent RNA polymerase that has a high specificity for bacteriophage T7 promoter sequences. The enzyme synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available. Application: Synthesis of labeled and unlabeled RNA transcripts (1). Source: Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid. Performance and Quality Testing: 3´ and 5´ exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a transcription reaction. Unit De...
Catalog: 18033-100
Size: 2 × 2,500 units

0.5-10 Kb RNA Ladder

Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > RNA Ladders

0.5-10 Kb RNA Ladder is suitable for sizing single-stranded RNA ranging from 0.5 kb to 10 kb in glyoxal or formaldehyde agarose gels. The ladder consists of nine bands in even increments (0.5, 1, 1.5, 2, 3, 4, 6, 8, and 10 kb) to simplify size estimation. The 1.5 kb band is more intense than the other bands, allowing easy orientation and identification of RNA bands on the gel. All RNA molecules in the ladder are polyA-tailed (40 nt) for convenient use as control templates for cDNA synthesis.
Catalog: 15623-200
Size: 75 µg

Breast Adenocarcinoma (MCF-7) Total RNA

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > Purified Total RNA

The highest-quality RNA available from Ambion®, it is DNase-treated and subjected to unsurpassed quality control standards. One tube containing 100 µg is provided at a concentration of 1 mg/mL. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA contamination in poly(A) preparations are all important aspects used to judge the quality of FirstChoice® RNAs. The samples are processed using Ambion® RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment is performed to ensure that the RNA is ready for use in any ...
Catalog: AM7846
Size: 100 µg

THE RNA Storage Solution

Category: Products & Services > Buffers & Chemicals > Nucleic Acid Storage & Nuclease Control Reagents > DNA & RNA Storage Reagents

THE Ambion® RNA Storage Solution is a buffer that provides greater RNA stability than standard 0.1 mM EDTA or TE Buffer. This 1 mM sodium citrate, pH 6.4 +/- 0.2 buffer is provided in one bottle containing 50 mL. The last step in every RNA isolation protocol is to resuspend the purified RNA pellet. After painstakingly isolating the RNA, it is crucial that the pellet be suspended and stored in a safe, RNase-free solution. THE RNA Storage Solution has two features that minimize base hydrolysis of RNA: a low pH, and sodium citrate, which is an efficient chelating agent. THE RNA Storage Solution i...
Catalog: AM7001
Size: 50 ml

Human Liver Total RNA

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > Purified Total RNA

The highest-quality RNA available from Ambion®, it is DNase-treated, subjected to unsurpassed quality control standards, and certified to contain small RNAs (miRNA, siRNA, and snRNA). One tube containing 100 µg is provided at a concentration of 1 mg/mL. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA contamination in poly(A) preparations are all important aspects used to judge the quality of FirstChoice® RNAs. The samples are processed using Ambion® RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment ...
Catalog: AM7960
Size: 100 µg

PureLink® Viral RNA/DNA Mini Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Viral RNA⁄DNA Kits

The PureLink® Viral RNA/DNA Mini Kit is a nucleic acid purification system designed for fast and easy isolation of viral RNA or DNA from cell-free samples, such as serum, plasma, and cerebrospinal fluid. Starting sample volumes can be as high as 500 µl and elution volumes as low as 10 µl, allowing for a 50-fold concentration of nucleic acids. The higher target concentration obtained using the PureLink® Viral RNA/ DNA Mini Kit provides greater sensitivity and accuracy in downstream detection procedures such as real-time PCR or endpoint analysis. Specially formulated buffers enable the purificat...
Catalog: 12280-050
Size: 50 preps

RNA later ®-ICE (25 ml bottle)

Category: Products & Services > Buffers & Chemicals > Nucleic Acid Storage & Nuclease Control Reagents > DNA & RNA Storage Reagents

RNAlater®-ICE is a novel reagent for transitioning frozen tissue to a state that is easily processed for extraction of high quality RNA. Frozen tissues are simply submerged in RNAlater®-ICE and allowed to thaw overnight at -20°C. Once thawed, the tissues can be processed like fresh tissues using standard RNA isolation procedures. No more laborious grinding of frozen tissue to safeguard the RNA. • Process previously frozen tissues like freshly harvested samples • Thawing tissues in RNAlater®-ICE protects RNA from degradation • No more tissue pulverizing with mortar and pestle and awkward transf...
Catalog: 4427575
Size: 10 bottles

0.1-2 Kb RNA Ladder

Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > RNA Ladders

0.1-2 Kb RNA Ladder is suitable for sizing single-stranded RNA from 0.1 to 2 kb in glyoxal or formaldehyde agarose gels. The ladder consists of ten bands in even increments (0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.8, 1.0, 1.5, and 2.0 kb) to simplify size estimation. The 0.3 kb band is more intense than the other bands, allowing easy orientation and identification of RNA bands on the gel. All RNA molecules in the ladder are polyA-tailed (40 nt) for convenient use as control templates for cDNA synthesis.
Catalog: 15623-100
Size: 75 µg

Illumina® TotalPrep™ RNA Amplification Kit

Category: Products & Services > Kits & Assays > RNA Amplification Kits

The Ambion® Illumina® TotalPrep™ RNA Amplification Kit (patent pending) is a complete system for generating biotinylated, amplified RNA for hybridization with Illumina Sentrix® arrays. The new TotalPrep™ RNA Amplification Kit incorporates extensive improvements to the in vitro transcription reaction (IVT) over the previous Illumina® RNA Amplification Kit. Experience these Improvements: • Improved first-strand cDNA synthesis using ArrayScript™ reverse transcriptase • Biotinylated UTP allows ease of use and optimal labeling • Maximization of cRNA yield using MEGAscript® IVT technology • Signific...
Catalog: AMIL1791
Size: 24 reactions

TaqMan® OpenArray® Genotyping Plate, Custom Format 192 QuantStudio™ 12K Flex

Category: Products & Services > Oligos, Primers, Probes & Nucleotides

TaqMan® OpenArray® Genotyping Plate, Custom Format 192 QuantStudio™ 12K Flex
Catalog: 4471117
Size: 10 pack

ERCC RNA Spike-In Mix

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

Variation in RNA expression data can be attributed to a variety of factors including the quality of the starting material, the level of cellularity and RNA yield, the platform employed, and the person performing the experiment. To control for these sources of variability, a common set of external RNA controls has been developed by the External RNA Controls Consortium (ERCC), an ad-hoc group of academic, private, and public organizations hosted by the National Institute of Standards and Technology (NIST). The controls consist of a set of unlabeled, polyadenylated transcripts designed to be adde...
Catalog: 4456740
Size: 1 kit