Culture Products
The combination of the antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective action against gram-positive and gram-negative bacteria, respectively. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus . Streptomycin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibit...
Catalog: 15070-063
Size: 100 ml
Size: 100 ml
Fungizone® antimycotic is a formulation of Amphotericin B with sodium deoxycholate as an emulsifying agent to maintain the suspension. Amphotericin B is used to prevent the growth of fungi by causing an increase in fungal plasma membrane permeability. Gibco® Fungizone® antimycotic is used for the prevention of yeast and multicellular fungi contamination of cell cultures. Gibco® Fungizone contains 250 µg of amphotericin B and 205 µg of sodium deoxycholate per ml in distilled water. The recommended working concentration ranges from 0.25 to 2.50 µg/ml. Life Technologies offers a variety of antibi...
Catalog: 15290-018
Size: 20 ml
Size: 20 ml
Recombinant Mouse Fibroblast Growth Factor Basic (also known as FGF Basic, bFGF, FGF 2, FGF-β or Heparin Binding Growth Factor) is a bioactive protein intended for use in cell culture applications. FGF-basic is involved in a number of biological processes including embryonic development and differentiation, neuron differentiation, survival, and regeneration, and the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. • High purity —no interference from other proteins or contaminants • High biological activity —more results with less...
Catalog: PMG0031
Size: 100 µg
Size: 100 µg
The CultureWell cell culture systems provide an integrated set-up for preparing cultured cells for staining and imaging. Each system uses medical-grade silicone gaskets preassembled with standard optical-quality coverslips in cell culture plates. The entire system is provided sterile and ready to use.
Catalog: C-24760
Size: 1 set
Size: 1 set
Total Exosome Isolation (from cell culture media) enables fast and efficient enrichment of intact exosomes from cell culture media samples. • Maximize recovery of intact exosomes for any type of downstream application • Easily isolate exosomes using our simple and reliable protocol • Avoid time-consuming ultra-centrifugation • Maximize flexibility—can be scaled up or down depending on the sample size Isolation of exosomes can be a tedious, non-specific, and difficult process. Total Exosome Isolation (from cell culture media) enables simple and reliable concentration of intact exosomes from cel...
Catalog: 4478359
Size: 50 ml
Size: 50 ml
Exosome – Human CD63 Isolation/Detection enables the purification of CD63+ exosomes (also called extracellular vesicles and multi-vesicular bodies) from pre-enriched cell culture samples. These exosomes can then be detected using techniques such as flow cytometry, electron microscopy, or Western blotting. The exosomes must be pre-enriched before isolation. This can be done by ultracentrifugation, or by using the fast and efficient Total Exosome Isolation (from cell culture media) reagent. • Obtain highly pure CD63+ exosomes • "See" your sample during handling • Easily scalable protocol • Detec...
Catalog: 106-06D
Size: 3 ml
Size: 3 ml
Collagen I, Rat Tail is the cell culture workhorse of basic coating and gel preparations for growing attachment cells. Gibco® Collagen I, Rat Tail is now available in pre-coated tissue culture plates to help save time at the bench. Designed to meet a number of different applications, our high quality, consistent Collagen I Coated Plates come in 3 formats – 96 well, 24 well and 6 well plates . • Ready to use, room temperature storage • Quality tested to help ensure consistency • Available in 3 different plate formats and in custom formats for a broad range of applications Easy to use, easy to s...
Catalog: A11428-02
Size: 5 plates
Size: 5 plates
Gibco® Trypsin 1:250 powder is an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength it is widely used for cell dissociation, during routine cell culture passaging, and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing Quality Testing Gibco® Trypsin 1:250 is tested for endotoxin, mycoplasma, bacterial, fungal and viral contamination. Other quality tests include multiple activity assays, ash analysis and mois...
Catalog: 27250-018
Size: 100 g
Size: 100 g
Catalog: 00-3002
Size: 100 tablets
Size: 100 tablets
Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Life Technologies offers a variety of Gibco® PBS formulations for a range of cell culture applications. Find the right formulation using the media selector tool . This PBS is manufactured as follows: Without • Calcium • Magnesium • Phenol Red
Catalog: 10010-023
Size: 500 ml
Size: 500 ml
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES
Catalog: 11960-069
Size: 10 × 500 ml
Size: 10 × 500 ml
PLUS™ Reagent is used in conjunction with transfection reagents, such as Lipofectamine™, to enhance transfection in adherent cell lines. Some customers find when using Plus™ Reagent that less optimization of DNA concentration is needed to achieve high transfection performance. The addition of Plus™ Reagent may also reduce the quantities of DNA and lipid required by approximately one-half compared to lipid alone. PLUS™ Reagent was specifically designed to expand the array of transfectable cell types (1). PLUS™ Reagent used with Lipofectamine™ Reagent has been demonstrated to improve the transfe...
Catalog: 11514-015
Size: 0.85 ml
Size: 0.85 ml
Gibco® MEM Vitamin Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Vitamin Solution is formulated to contain 100x the vitamins found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Vitamin Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001...
Catalog: 11120-052
Size: 100 ml
Size: 100 ml
Gibco® Neomycin is produced by Streptomyces fradiae . Polymyxin B Sulfate acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Gibco® Neomycin Sulfate is used for the prevention of bacterial contamination of cell cultures. This antibiotic is highly active against gram-negative bacteria. The recommended working concentration is 50 µg/ml. Life Technologies offers a variety of antibiotics and antimycotics for cell culture applications. Product Use For Research Use Only: Not intended for animal or human diagnosti...
Catalog: 21810-031
Size: 100 g
Size: 100 g
Iscove's Modified Dulbecco's Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. Life Technologies offers a variety of Gibco® IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This IMDM is modified as follows: With Without • L-glutamine • Phenol Red • α-thioglycerol • 2-mercaptoethanol
Catalog: 21056-023
Size: 500 ml
Size: 500 ml
Dulbecco's Modified Eagle Medium : Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of Gibco® DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM / F-12 is modified as follows: With Without • L-glutamine • HEPES • Phenol Red
Catalog: 11320-082
Size: 10 × 500 ml
Size: 10 × 500 ml
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • Sodium Pyruvate • L-glutamine • HEPES • Phenol Red
Catalog: 11965-126
Size: 6 × 1000 ml
Size: 6 × 1000 ml
Gibco® Trypsin solutions are made from trypsin powder—an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing This Trypsin is modified as follows: With Without • EDTA • Phenol Red
Catalog: 15400-054
Size: 100 ml
Size: 100 ml
Gibco® HAT Supplement is a liquid mixture of sodium hypoxanthine (5 mM), aminopterin (20 µM) and thymidine (0.8 mM). HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT–myeloma cells. Hypoxanthine and thymidine supply preformed purines and pyrimidines for DNA synthesis by hybridomas via the salvage pathway that utilizes HGPRT- contributed by the fused spleen cell. Aminopterin, a folic acid antagonist, inhibits the de novo nucleoside biosynthesis pathway. Product Use For Research Use Only. Not for use in diagnostic procedures. Dual site cGMP Manufac...
Catalog: 21060-017
Size: 100 ml
Size: 100 ml
Gibco® Zeocin™ selective reagent is a formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus . Gibco® Zeocin™ selective reagent acts by binding to and cleaving DNA, leading to cell death. Gibco® Zeocin™ selective reagent is used for selection of yeast, bacterial and mammalian cell cultures. This product is supplied at 100 mg/ml in sterile water and is used at a working concentration ranging between 50 - 400 µg/ml depending on the cell type. Life Technologies offers a variety of antibiotics and antimycotics for cell culture applications. Product Use For Res...
Catalog: R250-05
Size: 50 ml
Size: 50 ml
Culture Products
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