Coli Competent
BL21(DE3)pLysE E. coli are ideal for use with T7 promoter-based expression vectors. Like BL21(DE3)pLysS E. coli , BL21(DE3)pLysE carry the lambda DE3 lysogen. In addition, BL21(DE3)pLysE cells contain the pLysE plasmid, which constitutively expresses T7 lysozyme. T7 lysozyme reduces the basal expression of target genes by inhibiting T7 RNA polymerase. The pLysE plasmid in BL21(DE3)pLysE expresses higher levels of T7 lysozyme than the pLysS plasmid in BL21(DE3)pLysS. The BL21(DE3)pLysE strain provides tighter control of T7 RNA polymerase, which is necessary when the recombinant protein to be ex...
Catalog: C6565-03
Size: 20 × 50 µl
Size: 20 × 50 µl
The One Shot® Stbl3™ E. coli strain is designed especially for cloning direct repeats found in lentiviral expression vectors. Unlike TOP10 E. coli , these cells reduce the frequency of homologous recombination of long terminal repeats (LTRs) found in ViraPower™ Lentiviral Expression Vector and other retroviral vectors. The DNA yield from Stbl3™ cells is often >10-fold higher than alternative strains such as TOP10 (Table 1). One Shot® Stbl3™ Chemically Competent Cells deliver: • Optimal performance for the propagation of lentiviral vectors • Enhanced genomic cloning capacity ( mcr B, mrr ) • Ex...
Catalog: C7373-03
Size: 20 × 50 µl
Size: 20 × 50 µl
TOP10 E. coli are provided at a transformation efficiency of 1 x 10e 9 cfu/µg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids. The genotype of TOP10 cells is similar to the DH10B™strain, and offers the following features: -hsd R for efficient transformation of unmethylated DNA from PCR amplifications -mcrA for efficient transformation of methylated DNA from genomic preparations -lacZΔM15 for blue/white color screening of recombinant clones -endA1 for cleaner preparations of DNA and better results in d...
Catalog: C4040-10
Size: 10 reactions
Size: 10 reactions
One Shot® BL21 Star™ (DE3) E. coli are chemically competent cells designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems (e.g., Champion™ pET vectors) . One Shot® BL21 Star™ (DE3) Chemically Competent cells are controlled for a transformation efficiency of 1 x 10 8 cfu/ µg plasmid DNA. • A genotype that promotes high mRNA stability and protein yield • Ideal for use with low copy number, T7 promoter-based plasmids • The single tube, single use format allows all transformation steps to take place i...
Catalog: C6010-03
Size: 20 reactions
Size: 20 reactions
The Echo™ Cloning System is a two-vector system that utilizes the Cre-lox site-specific recombination system of bacteriophage P1 (1,2) for rapid cloning into multiple vectors. Your gene of interest is cloned into one of the specially designed donor vectors which can then be recombined with as many different Echo™ acceptor vectors as desired. The donor and acceptor vectors are placed in a buffer that contains Cre recombinase for a 25-minute, in vitro recombination reaction. Cre binds to the lox site present on each vector, brings them together, then cleaves and covalently reattaches the DNA str...
Catalog: C1010-10
Size: 10 reactions
Size: 10 reactions
BL21(DE3) E. coli are ideal for use with bacteriophage T7 promoter-based expression systems ( e.g. , pRSET, pCR¤T7, and pET). BL21(DE3) carry the lambda DE3 lysogen. Recombinant proteins that are non-toxic to E. coli are generally expressed at higher levels in BL21(DE3) cells than in BL21(DE3)pLysS or BL21(DE3)pLysE. However, the basal expression levels of heterologous genes are significantly higher in BL21(DE3) than in BL21(DE3)pLysS or BL21(DE3)pLysE.
Catalog: C6000-03
Size: 20 × 50 µl
Size: 20 × 50 µl
One Shot® BL21-AI™ E. coli are chemically competent cells designed for applications that require tight regulation and strong expression of toxic proteins from any T7 promoter-based expression systems. One Shot® BL21-AI™ Chemically Competent cells have a transformation efficiency of >1×10 8 cfu/ µg plasmid DNA. • Ideal for inducing expression of toxic protein • Gene expression is regulated by the addition of L-arabinose to culture • Suitable for use with any T7 promoter-based plasmids • Supplied in a convenient One Shot® format Excellent Expression of Toxic Recombinant Proteins One Shot® BL21-A...
Catalog: C6070-03
Size: 20 × 50 µl
Size: 20 × 50 µl
One Shot® TOP10 E. coli are convenient to use chemically competent cells quality controlled for a transformation efficiency of 1 x 10 9 cfu/ µg plasmid DNA. One Shot® TOP10 Chemically Competent cells are the same competent cells that come with many of our cloning kits and are ideal for high-efficiency cloning and plasmid propagation. • Maximizes cloning efficiency in single tube format • Enhanced genomic DNA cloning capabilities Easy-to-use, Single Tube Format One Shot® TOP10 E. coli are provided at a transformation efficiency of 1 x 10 9 cfu/ µg supercoiled DNA and allow stable replication of...
Catalog: C4040-06
Size: 40 reactions
Size: 40 reactions
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Catalog: K100-01
Size: 20 reactions
Size: 20 reactions
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Catalog: K101-01
Size: 20 reactions
Size: 20 reactions
Zero Blunt® TOPO® PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR®-Blunt II-TOPO® Vector for 5-minute benchtop ligations without ligase (1). The pCR®-Blunt II-TOPO® Vector also features: • ccdB gene for positive selection (2) • Eco R I sites flanking the PCR product insertion site for easy excision of inserts • Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli • SP6 promoter/primer site for...
Catalog: K2800-02
Size: 20 preps
Size: 20 preps
The pENTR™/SD/D-TOPO® Cloning Kits utilize a highly efficient, 5-minute cloning strategy ("TOPO® Cloning") to directionally clone a blunt-end PCR product into a vector for entry into the Gateway® System or the MultiSite Gateway® System. Blunt-end PCR products clone directionally into the pENTR™/SD/D-TOPO® entry vector at greater than 90% efficiency, and include an upstream Shine-Dalgarno sequence for expression in prokaryotes. The kit comes with everything necessary to clone and select your PCR amplified gene of interest. Gateway® System Ready - Rapidly shuttle cloned genes between multiple ve...
Catalog: K2420-20
Size: 20 reactions
Size: 20 reactions
The TOPO® TA Cloning® Kits for Sequencing provide a highly efficient, 5 minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing. Each kit uses the pCR™4-TOPO® TA vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction. These kits include everything necessary to clone and select recombinant vectors containing your PCR fragment of choice. Get More Sequence— Allows for more insert sequence and less vector seuquence when usin...
Catalog: K4530-20
Size: 20 reactions
Size: 20 reactions
Coli Competent
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