Co2 Independent Medium without L Glutamine

William's E Medium, no Glutamine

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Williams' Media E

William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. This media can also be used for growing hepatocyte cells of different species, such as human HepaRG™ cells. This William's E Medium is modified as follows: With Without • Phenol Red • L-glutamine • HEPES
Catalog: 12551-032
Size: 500 ml

MCDB 131 Medium, no Glutamine

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > MCDB 131

MCDB 131 Medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular endothelial cells (HUVEC). MCDB 131 Medium is also used with other cell types, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells. This MCDB 131 Medium is manufactured as follows: With Without • Phenol Red • L-glutamine • Sodium pyruvate • HEPES
Catalog: 10372-019
Size: 500 ml

Keratinocyte-SFM Medium (Kit) with L-Glutamine, EGF and BPE

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Keratinocyte-SFM

with L-Glutamine, EGF and BPE. Shelf life after supplementation: 2 weeks
Catalog: 17005-075
Size: 1 kit

Opti-MEM® Reduced Serum Medium, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Opti-MEM® I

Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications. This Opti-MEM® is modified ...
Catalog: 11058-021
Size: 500 ml

DMEM, Low Glucose, Pyruvate, no Glutamine, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • Low Glucose • L-glutamine • Sodium Pyruvate • Phenol Red • HEPES
Catalog: 11054-001
Size: 1 L

Medium 199, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Media 199

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. Life Technologies offers a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This M199 is modified as follows: With Without • L-glutamine • Phenol Red • HEPES
Catalog: 11043-023
Size: 500 ml

DMEM, High Glucose, no Glutamine

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES
Catalog: 11960-069
Size: 10 × 500 ml

imMedia™ Growth Medium, agar, ampicillin

Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media

imMedia™ growth medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia™ growth medium contains everything you need to prepare low-salt LB medium or agar plates. You'll save hours of medium preparation time because there is: • No weighing or mixing of media components • No autoclaving • No waiting for media to cool before adding antibiotics Fast Medium Preparation imMedia™ growth medium is fast food for E. coli . Simply mix with water in a clean flask, heat in a microwave oven, and in less than 5 minutes your medium is pr...
Catalog: Q601-20
Size: 20 each

CD Hybridoma Medium (1X), Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Hybridoma Media

Gibco® CD Hybridoma Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® CD Hybridoma Medium features: • Ability to support suspension and stationary hybridomas and myelomas • Formulated without L-glutamine for stability • Animal origin-free, protein-free and chemically defined formulation Ability to support suspension and stationary hybridomas and myelomas Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® CD Hybridoma Medi...
Catalog: 11279-023
Size: 1,000 ml

Gibco® FreeStyle™ 293 Expression Medium

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > 293 Media

FreeStyle™ 293 Expression Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth and transfection of 293-F cells in suspension without adaptation. The medium is complete and ready-to-use, with no supplementation required. Gibco® FreeStyle™ 293 Expression Medium features: • Complete and ready-to-use format containing GlutaMAX™ supplement • Ability to support suspension growth and transfection of 293-F cells • Animal origin-free, protein-free and chemically defined formulation • Easy use as part of the FreeStyle™ 293 ...
Catalog: 12338-018
Size: 1000 ml

MEM, NEAA, no Glutamine

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Minimum Essential Media (MEM)

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • NEAA • L-glutamine • Phenol Red • HEPES
Catalog: 10370-088
Size: 10 × 500 ml

MEM, HEPES, no Glutamine

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Minimum Essential Media (MEM)

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • HEPES • L-glutamine • Phenol Red
Catalog: 12360-038
Size: 500 ml

Opti-MEM® Reduced Serum Medium

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Opti-MEM® I

Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications. This Opti-MEM® is modified ...
Catalog: 31985-070
Size: 500 ml

DMEM, High Glucose, no Glutamine, no methionine, no cystine

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES • L-methio...
Catalog: 21013-024
Size: 500 ml

CD CHO Medium (1X), Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > CHO & DG44 Media

CD CHO medium is a protein-free, serum-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture. With a proven track record of quality for more than 10 years, CD CHO medium contains no proteins or peptide components of animal, plant, or synthetic origin, as well as no undefined lysates or hydrolysates. When to Use CD CHO Medium • Your GS CHO cells are recommended to be grown in CD CHO medium • Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a reg...
Catalog: 10743-029
Size: 1,000 ml

CD CHO Medium (1X), Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > CHO & DG44 Media

CD CHO medium is a protein-free, serum-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture. With a proven track record of quality for more than 10 years, CD CHO medium contains no proteins or peptide components of animal, plant, or synthetic origin, as well as no undefined lysates or hydrolysates. When to Use CD CHO Medium • Your GS CHO cells are recommended to be grown in CD CHO medium • Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a reg...
Catalog: 10743-002
Size: 20 L