Cloning Competent Cells & Strains
Library Efficiency® DH5α™ Competent Cells are a versatile strain of chemically competent cells that demonstrate transformation efficiencies of >1 x 10 8 cfu/ µg plasmid DNA. Library Efficiency® DH5α™ Competent Cells are ideal for difficult cloning constructions or any application that uses limiting amounts of DNA. Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal Maintains plasmid DNA with minimum recombination events Delivers high yield plasmid preparations for downstream applications M13mp cloning vectors may be propagated on a lawn of DH5α-FT™ , DH5αF...
Catalog: 18263-012
Size: 1 ml
Size: 1 ml
MAX Efficiency® Stbl2™ Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts. In addition to rec A1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences (1) and tandem array genes (2). MAX Efficiency® Stbl2™ Competent Cells offer: >1 x 10 9 transformants/flg for cloning rare sequences Elimination of mcr BC- hsd RMS- mrr and mutation of mcr A allows cloning of methylated genomic sequences (1) Unique genotype stabilizes direct repeat and retroviral sequences such as SIV and HIV (1) Greatly ...
Catalog: 10268-019
Size: 1 ml
Size: 1 ml
MAX Efficiency® DH10Bac™ Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac® Baculovirus Expression System . The DH10Bac™ E. coli strain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac™, to create an expression bacmid containing a cloned gene of interest. Features of MAX Efficiency® DH10Bac™ Competent Cells include: • Consistent yields of >1 × 10 8 transformants/ µg • Tolerance of small amounts of undiluted ligation reactions • Support of efficient blue/white colony screening (Φ80 lac ZΔM15 marker) About MAX Ef...
Catalog: 10361-012
Size: 5 × 100 µl
Size: 5 × 100 µl
One Shot® ccd B Survival™ 2 T1 Phage-Resistant (T1 R ) chemically competent E. coli are suitable for the propagation of plasmids containing the ccd B gene, and are designed for use with the Gateway® Vector Conversion System and for propagating Gateway® destination, donor, and super-coiled entry vectors. Transformation efficiency is >1 × 10 9 transformants/ µg DNA. The ccd B Survival™ 2 T1 R E. coli strain is derived from the TOP10 strain. The ccdB Survival™ 2 T1 R E. coli strain offers the following Features: • Resistance to the ccd B gene product • Resistance to T1 and T5 phage (the ton A/ fh...
Catalog: A10460
Size: 10 transformations
Size: 10 transformations
ElectroMAX™ DH10B™ T1 Phage-Resistant electrocompetent E. coli cells offer transformation efficiencies that are among the highest achieved by any product offered by Life Technologies, and can exceed 1 x 10 10 cfu/ µg plasmid DNA in efficiency. They are ideal for applications requiring high efficiency transformation, including cDNA and gDNA library construction. • Maximizes yield of transformants from limited cloning products • Permits efficient cloning of methylated DNA • Resistant to T1 and T5 bacteriophages • Supports blue/white screening by α-complementation on plates containing X-Gal or Bl...
Catalog: 12033-015
Size: 0.5 ml
Size: 0.5 ml
One Shot® TOP10 E. coli are convenient to use chemically competent cells quality controlled for a transformation efficiency of 1 x 10 9 cfu/ µg plasmid DNA. One Shot® TOP10 Chemically Competent cells are the same competent cells that come with many of our cloning kits and are ideal for high-efficiency cloning and plasmid propagation. • Maximizes cloning efficiency in single tube format • Enhanced genomic DNA cloning capabilities Easy-to-use, Single Tube Format One Shot® TOP10 E. coli are provided at a transformation efficiency of 1 x 10 9 cfu/ µg supercoiled DNA and allow stable replication of...
Catalog: C4040-06
Size: 40 reactions
Size: 40 reactions
The One Shot® Stbl3™ E. coli strain is designed especially for cloning direct repeats found in lentiviral expression vectors. Unlike TOP10 E. coli , these cells reduce the frequency of homologous recombination of long terminal repeats (LTRs) found in ViraPower™ Lentiviral Expression Vector and other retroviral vectors. The DNA yield from Stbl3™ cells is often >10-fold higher than alternative strains such as TOP10 (Table 1). One Shot® Stbl3™ Chemically Competent Cells deliver: • Optimal performance for the propagation of lentiviral vectors • Enhanced genomic cloning capacity ( mcr B, mrr ) • Ex...
Catalog: C7373-03
Size: 20 × 50 µl
Size: 20 × 50 µl
TOP10 E. coli are provided at a transformation efficiency of 1 x 10e 9 cfu/µg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids. The genotype of TOP10 cells is similar to the DH10B™strain, and offers the following features: -hsd R for efficient transformation of unmethylated DNA from PCR amplifications -mcrA for efficient transformation of methylated DNA from genomic preparations -lacZΔM15 for blue/white color screening of recombinant clones -endA1 for cleaner preparations of DNA and better results in d...
Catalog: C4040-10
Size: 10 reactions
Size: 10 reactions
MC1061/P3 competent E. coli are used for highly efficient transformation of vectors that require the P3 episome for selection and maintenance (i.e., pCDM8, pcDNA™1.1, or any other sup F-containing vector).
Catalog: C663-03
Size: 1.5 ml
Size: 1.5 ml
The OmniMAX™ 2 T1 Phage-Resistant (T1 R ) E. coli strain is an improved chemically competent cell line, perfect for use in all cloning applications, including the Gateway® Technology. OmniMAX™ 2 -T1 R cells offer the highest transformation efficiency of any chemically competent cells in a One Shot® format with >5 x 10 9 transformants/µg pUC19. They also provide efficient transformation of highly methylated DNA, since OmniMAX™ 2 -T1 R cells lack the E. coli K12 restriction systems (mc rA Δ (mrr hs dRMS- mc rBC)). In addition, the strain carries the to nA genotype, which confers resistance to T1...
Catalog: C8540-03
Size: 20 transformations
Size: 20 transformations
Cloning Competent Cells & Strains
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