Chromatographic Reversed-Phase Columns & Media
Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS® CaptureSelect® FSH HPLC columns incorporate the advantages of high-speed POROS® chromatography resins with the unique selectivity of CaptureSelect® ligands, enabling 3-minute quantitation of human follicle stimulating hormone (FSH). Feature...
Catalog: 4481828
Size: 1 each
Size: 1 each
Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS® CaptureSelect® FSH HPLC columns incorporate the advantages of high-speed POROS® chromatography resins with the unique selectivity of CaptureSelect® ligands, enabling 3-minute quantitation of human follicle stimulating hormone (FSH). Feature...
Catalog: 4481822
Size: 1 each
Size: 1 each
Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS® CaptureSelect® FSH HPLC columns incorporate the advantages of high-speed POROS® chromatography resins with the unique selectivity of CaptureSelect® ligands, enabling 3-minute quantitation of human follicle stimulating hormone (FSH). Feature...
Catalog: 4481826
Size: 1 each
Size: 1 each
Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS® CaptureSelect® FSH HPLC columns incorporate the advantages of high-speed POROS® chromatography resins with the unique selectivity of CaptureSelect® ligands, enabling 3-minute quantitation of human follicle stimulating hormone (FSH). Feature...
Catalog: 4481824
Size: 1 each
Size: 1 each
GoPure™ 0.5 cmD pre-packed columns are intended for the screening of POROS® ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features: • Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform • Direct connection to standard chromatography systems • Packed with POROS® high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates The resin in this GoPure™ D 50 µm Column has a dimethylaminopropyl functional group for stro...
Catalog: 4481319
Size: 1 column
Size: 1 column
GoPure™ 0.5 cmD pre-packed columns are intended for the screening of POROS® ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features: • Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform • Direct connection to standard chromatography systems • Packed with POROS® high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates The resin in this GoPure™ HQ 50 µm Column has a quaternized polyethyleneimine functional gro...
Catalog: 4481315
Size: 1 column
Size: 1 column
GoPure™ 0.5 cmD pre-packed columns are intended for the screening of POROS® ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features: • Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform • Direct connection to standard chromatography systems • Packed with POROS® high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates The resin in this GoPure™ PI 50 µm Column has a polyethyleneimine functional group for stron...
Catalog: 4481318
Size: 1 column
Size: 1 column
GoPure™ 0.5 cmD pre-packed columns are intended for the screening of POROS® ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features: • Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform • Direct connection to standard chromatography systems • Packed with POROS® high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates The resin in this GoPure™ HS 50 µm Column has a sulphopropyl functional group for strong cat...
Catalog: 4481316
Size: 1 column
Size: 1 column
GoPure™ 0.5 cmD pre-packed columns are intended for the screening of POROS® ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features: • Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform • Direct connection to standard chromatography systems • Packed with POROS® high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates The resin in this GoPure™ XS Column has a sulphopropyl functional group for strong cation ex...
Catalog: 4481317
Size: 1 column
Size: 1 column
Glutathione agarose prepared by linking the sulfur atom of glutathione to crosslinked beaded agarose can be used for purification of GST fusion proteins. Each milliliter of gel can bind approximately 5-6 mg of boivine-liver GST. Adding excess free glutathione liberates the GST fragment from the matrix which can then be regenerated by washing with a high-salt buffer.
Catalog: G2879
Size: 10 ml
Size: 10 ml
Recombinant protein G is produced in a strain of E. coli which contains the gene for protein G from Streptococcus sp. This product is prepared by covalently coupling highly purified rec-Protein G to Sepharose® 4B. It is supplied as a 50% suspension containing 25 ml rec-Protein G-Sepharose® 4B + 25 ml PBS (10 mM phosphate-buffered saline, pH 7.4, containing 0.1% Proclin preservative). Applications: Immunoglobulin affinity purification and Immunoprecipitation. Recombinant protein G binds to the Fc region of many types of immunoglobulin G including: all four subclasses of human IgG mouse IgG rabb...
Catalog: 10-1243
Size: 25 ml
Size: 25 ml
Applied Biosystems® MultiScribe™ Reverse Transcriptase is a recombinant Moloney murine leukemia virus (rMoMuLV) reverse transcriptase. MultiScribe™ Reverse Transcriptase is similar to MuLV reverse transcriptase but differs in its recommended usage. MultiScribe™ Reverse Transcriptase is a recombinant RNA-dependent DNA polymerase that uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary DNA (cDNA) strand. 5000U supplied in 100 µl (50U/ µl). • Compatible with one-step and two-step RT-PCR reactions, which provides flexibility in assay set-up • Individual...
Catalog: 4311235
Size: 100 µl
Size: 100 µl
The microcentrifuge spin columns included with the FastTrack® 2.0 and Micro-FastTrack™ 2.0 Kits are available separately. The columns are made from polypropylene and are designed to enhance the speed and efficiency of polyA + RNA isolation. The columns are sterilized and RNase-free.
Catalog: R500-20
Size: 200 columns
Size: 200 columns
SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A) + RNA sample. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity...
Catalog: 18080-044
Size: 10,000 units
Size: 10,000 units
SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics. • Yield – Reduced RNase H activity results in greater first-strand cDNA yields • Size of ...
Catalog: 18064-071
Size: 4 × 10,000 units
Size: 4 × 10,000 units
Applied Biosystems® MuLV Reverse Transcriptase is a recombinant RNA-dependent DNA polymerase that uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary DNA (cDNA) strand. It can be used for the preparation of cDNA libraries, or for first strand synthesis in RT-PCR, or in dideoxy sequencing reactions in place of Klenow enzyme for G+C-rich regions. MuLV Reverse Transcriptase lacks endonuclease activity and has extremely low RNase H activity compared to AMV reverse transcriptase.
Catalog: N8080018
Size: 5000 units
Size: 5000 units
Cloned Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT) is highly purified from insect cells infected with baculovirus containing the pol gene of AMV (1). The resulting enzyme has a higher specific activity than does native AMV RT, providing enhanced cDNA yields and improved sensitivity. The enzyme, accompanying buffers, and protocols are optimized to synthesize first-strand cDNA for amplification by PCR, and for cloning double-stranded cDNA of up to 12 kb (Figure 2). AMV RT has endoribonuclease H activity. • Flexibility —Works with oligo(dT), random primers, or a gene-specific prim...
Catalog: 12328-019
Size: 750 units
Size: 750 units
Custom Media: 2-MERCAPTOETHANOL
Catalog: A13558SA
Size: 1 each
Size: 1 each
Custom Media: MAGNESIUM CHLORIDE ANHYDROUS
Catalog: A13735IN
Size: 1 each
Size: 1 each
Custom Media: N2B27 CUSTOM MEDIA
Catalog: ME100137L1
Size: 1 each
Size: 1 each
Chromatographic Reversed-Phase Columns & Media
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