Chemiluminescence-Based Assay

Luminescence Enhancer For Membrane Blotting Assays, Nitro-Block-II™ Enhancer

Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Chemiluminescent Substrates

This concentrated Nitro-Block-II™ Enhancer can be used with CSPD® or CDP-Star® chemiluminescent substrates to increase the alkaline phosphatase signal for protein or nucleic acid blots. The Nitro-Block-II™ membrane enhancer increases chemiluminescent signal intensity on nitrocellulose and PVDF membranes. • Chemically optimized formulations provide enhanced signal intensity in blotting applications with dioxetane-based substrate solutions (CSPD® or CDP-Star® substrates) on nitrocellulose or PVDF membranes. • Enhancer increases signal intensity, allowing decreased signal acquisition time. Increa...

Catalog: T2184
Size: 20 ml

MitoProbe™ DiIC 1 (5) Assay Kit - For Flow Cytometry - 100 Assays

Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents

The MitoProbe™ DiIC 1 (5) Assay Kit provides solutions of the membrane-potential-sensitive cyanine dye DiIC 1 (5), and a mitochondrial membrane-potential disrupter, CCCP, for the study of mitochondrial membrane potential. DiIC 1 (5) penetrates the cytosol of eukaryotic cells and accumulates primarily in mitochondria with active membrane potentials, producing bright, far-red fluorescence. DiIC 1 (5) staining intensity decreases with decreased mitochondrial membrane potential.

Catalog: M34151
Size: 1 kit

Tango™ CCR5- bla U2OS Cells

Category: Products & Services > Kits & Assays > Cell Based Assay Kits > GPCR Cell Based Assay Kits

For Academic pricing please login or contact us at discoverysciences@invitrogen.com. The Tango™ CCR5- bla U2OS cells contain the human Chemokine (C-C Motif) Receptor 5 (CCR5) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR- bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase ( bla ) reporter gene under the control of a UAS response element. The Tango™ CCR5- bla U2OS cells are functionally validated for Z' and EC 50 concentrations of MIP-1a. In addition,...

Catalog: K1788
Size: 8 × 10^6 cells

GeneBLAzer® Jurkat Control Kit

Category: Products & Services > Kits & Assays > Cell Based Assay Kits > Pathway Analysis Cell Based Assay Kits

Th e GeneBLAzer® Jurkat Control Kit contains constitutively expressing (CMV- bla ) and wildtype Jurkat cells to provide positive and negative controls, respectively, for evaluating beta-lactamase activity (Figure 1).

Catalog: K1206
Size: 1 kit

TaqMan® GTXpress™ Master Mix, Mini-Pack (1 mL)

Category: Products & Services > Kits & Assays > Genotyping Kits > SNP Genotyping Kits

The TaqMan® GTXpress™ Master Mix delivers accurate genotyping results with robust performance in less than 50 min. Applied Biosystems® TaqMan® GTXpress™ Master Mix (2X) combines AmpliTaq® Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format. The 1 mL pack includes reagents sufficient to perform 400 PCR reactions (5 µL/reaction). • Compatible with both Fast and Standard mode thermal cycling conditions • Pre-mixed components stored at 2°–8°C significantly reduce assay setup time • Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays •...

Catalog: 4403311
Size: 400 reactions

MicroSEQ® 500 16S rDNA PCR Kit with Protocol

Category: Products & Services > Kits & Assays > Microbial Detection & Identification Kits

The MicroSEQ® 500 16S rDNA PCR Kit is the PCR component of the MicroSEQ® 500 16S rDNA Bacterial Identification System, which provides an easy-to-use DNA sequence-based method to identify most bacteria. PCR-amplified products generated with this kit can be sequenced using the MicroSEQ® 500 16S rDNA Sequencing Kit. The two kits together permit the quick and convenient amplification and sequencing of 500 base pairs of the 16S rRNA gene, which can then be compared to the MicroSEQ® 16S rDNA 500 Library to obtain an identity match. This kit includes a copy of the protocol, and a Quick Reference Card...

Catalog: 4348229
Size: 1 kit w⁄ Protocol

Tali® Cell Cycle Kit

Category: Products & Services > Kits & Assays > Cell Analysis Kits > Cell Viability Kits

The Tali® Cell Cycle Kit includes a ready-to-use reagent that, when used with the Tali® Image-Based Cytometer, provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle. This single-addition reagent is composed of propidium iodide (PI), RNase A, and Triton X-100. The Tali® Cell Cycle Kit offers a simple workflow: • The pre-mixed reagent can be stored at room temperature and used when your cells are ready • Simply fix your cells, add the reagent, incubate, and visualize • Cell cycle data can be analyzed on the Tali® instrument or exported as a ....

Catalog: A10798
Size: 50 assays

BRAF Mutation Analysis Reagents

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Genetic & Chromosome Abnormality Detection Kits

The BRAF Mutation Analysis Reagents employ a simple fragment analysis workflow to detect 1% mutant to wild type genomic DNA across 3 common and rare mutations in the BRAF gene region (see Table 1). The BRAF set provides sufficient reagent for analysis of 30 samples. Advantages of the BRAF Mutation Analysis Reagents include: • Simple Analysis—unambiguous, easy-to-interpret results • Thorough Coverage—detects 3 mutations in the BRAF gene • Sensitive—detects mutations at frequencies as low as 1 to 5% against a wild type genomic DNA background • Efficient—interrogate multiple loci from the same sa...

Catalog: 4452136
Size: 1 kit