Chemical Fragmentation of DNA
Zero Blunt® TOPO® PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR®-Blunt II-TOPO® Vector for 5-minute benchtop ligations without ligase (1). The pCR®-Blunt II-TOPO® Vector also features: • ccdB gene for positive selection (2) • Eco R I sites flanking the PCR product insertion site for easy excision of inserts • Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli • SP6 promoter/primer site for...
Catalog: K2800-02
Size: 20 preps
Size: 20 preps
The pENTR™/SD/D-TOPO® Cloning Kits utilize a highly efficient, 5-minute cloning strategy ("TOPO® Cloning") to directionally clone a blunt-end PCR product into a vector for entry into the Gateway® System or the MultiSite Gateway® System. Blunt-end PCR products clone directionally into the pENTR™/SD/D-TOPO® entry vector at greater than 90% efficiency, and include an upstream Shine-Dalgarno sequence for expression in prokaryotes. The kit comes with everything necessary to clone and select your PCR amplified gene of interest. Gateway® System Ready - Rapidly shuttle cloned genes between multiple ve...
Catalog: K2420-20
Size: 20 reactions
Size: 20 reactions
The TOPO® TA Cloning® Kits for Sequencing provide a highly efficient, 5 minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing. Each kit uses the pCR™4-TOPO® TA vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction. These kits include everything necessary to clone and select recombinant vectors containing your PCR fragment of choice. Get More Sequence— Allows for more insert sequence and less vector seuquence when usin...
Catalog: K4530-20
Size: 20 reactions
Size: 20 reactions
DNA Grade Agarose has a high sieve capacity, providing optimal resolution of DNA during gel electrophoresis. The low sulfur content allows gels to be run faster without becoming brittle. Supplied as a powder that is reconstituted in 0.5X TBE for high-quality agarose gels.
Catalog: 75000-500
Size: 500 g
Size: 500 g
Ambion® DNA Zap ™ (patent pending) consists of two solutions that are innocuous when used alone, but become a potent nucleic acid degrading solution when mixed. This mixture is able to instantaneously degrade high levels of contaminating DNA and RNA from surfaces. 250 mL of each solution are provided. • Completely degrades contaminating DNA and RNA at the level of PCR sensitivity • Works on contact • Degrades nucleic acid, unlike other products on the market that only act as detergents • Ideal for cleaning PCR tubes, PCR machine surfaces, pipettors, lab benches, lab equipment, and microfuge tu...
Catalog: AM9890
Size: 250 ml
Size: 250 ml
UltraPure™ DNA Typing Grade® 50X TAE Buffer is a sterile-filtered solution of 2 M Tris-acetate and 50 mM EDTA. TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. To prepare a 1X solution, mix one volume of UltraPure™ 50X TAE Buffer with 49 volumes of distilled water. A 1X TAE Buffer solution contains 40 mM Tris-acetate and 1 mM EDTA with a pH of 8.3 at 23“C. Performance and Quality Testing: No DNase activity detected.
Catalog: 24710-030
Size: 1 L
Size: 1 L
Ambion® DNA Zap ™ (patent pending) consists of two solutions that are innocuous when used alone, but become a potent nucleic acid degrading solution when mixed. This mixture is able to instantaneously degrade high levels of contaminating DNA and RNA from surfaces. 250 mL of each solution are provided. • Completely degrades contaminating DNA and RNA at the level of PCR sensitivity • Works on contact • Degrades nucleic acid, unlike other products on the market that only act as detergents • Ideal for cleaning PCR tubes, PCR machine surfaces, pipettors, lab benches, lab equipment, and microfuge tu...
Catalog: AM9890M
Size: 250 ml (Manual)
Size: 250 ml (Manual)
Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 ml of 50 mM MgCl 2 . It is included with Platinum® Taq, Taq, and the SuperScript® First-Strand Synthesis System for RT-PCR.
Catalog: 18067-017
Size: 2 × 1 ml
Size: 2 × 1 ml
This purified recombinant human biotinylated Fab recognizes DNMT3A in human samples. Recombinant antibody characteristics include: • Validation in immunoprecipitation (IP) • Superior lot-to-lot consistency • Extremely high specificity and sensitivity • Recombinant human biotinylated Fab • Specific for DNMT3A DNMT3A (DNA (cytosine-5)-methyltransferase 3 alpha) is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner...
Catalog: 730089
Size: 100 µg
Size: 100 µg
GeneScan™ 1000 ROX™ Size Standard is an internal lane size standard developed for use with Applied Biosystems® fluorescence-based DNA electrophoresis systems. The use of an internal lane size standard during electrophoresis enables automated data analysis and precise DNA fragment size comparisons between electrophoresis runs. GeneScan™ 1000 ROX™ Size Standard is designed for sizing DNA fragments in the 47–946 bp range and provides labeled fragments of 47, 51, 55, 82, 85 93 99, 126, 136, 262, 293, 317, 439, 557, 692, 695 and 946 bases. Each of the DNA fragments is labeled with ROX™ fluorophore,...
Catalog: 401098
Size: 400 reactions
Size: 400 reactions
GeneScan™ 500 TAMRA™ Size Standard is an internal lane size standard developed for use with the Applied Biosystems fluorescence- based DNA electrophoresis systems. The use of an internal lane size standard during electrophoresis enables automated data analysis and precise DNA fragment size comparisons between electrophoresis runs. GeneScan™ 500 TAMRA™ Size Standard is designed for sizing DNA fragments in the 35-500 bp range and provides 16 single-stranded labeled fragments of 35, 50, 75, 100, 139, 150, 160, 200, 250, 300, 340, 350, 400, 450, 490, and 500 bases. Each of the DNA fragments is lab...
Catalog: 401733
Size: 800 reactions
Size: 800 reactions
The GeneScan™ 1200 LIZ® Size Standard is a fifth dye (LIZ®) labeled high density size standard for the reproducible sizing of fragment analysis samples. The standard contains 68 LIZ® dye-labeled, single-stranded DNA fragments. Since the standard is labeled with the fifth dye, users can genotype a greater number of markers in a given lane, compared to the four-dye system. GeneScan™ 1200 LIZ® Size Standard is designed for sizing DNA fragments in the 20-1200 nucleotides range and provides 68 single-stranded labeled fragments of: 20, 30, 40, 60, 80, 100, 114, 120, 140, 160, 180, 200, 214, 220, 240...
Catalog: 4379950
Size: 800 reactions
Size: 800 reactions
The GeneScan™ 500 LIZ® Size Standard is a fifth dye-labeled size standard for the reproducible sizing of fragment analysis data. GeneScan™ 500 LIZ® Size Standard is designed for sizing DNA fragments in the 35-500 nucleotides range and provides 16 single-stranded labeled fragment of: 35, 50, 75, 100, 139, 150, 160, 200, 250, 300, 340, 350, 400, 450, 490 and 500 nucleotides. The sizing curve generated from these fragments make the GeneScan™ 500 LIZ® Size Standard ideal for a variety of fragment analysis applications such as Microsatellites, Fragment Length Polymorphisms and Relative Fluorescent ...
Catalog: 4322682
Size: 800 reactions
Size: 800 reactions
Contains 45 mg 6-FAM™ Dye Phosphoramidite. 6-FAM™ Dye Phosphoramidite is used for the 5’ attachment of FAM™ dye during oligonucleotide synthesis. 6-FAM™ Dye Phosphoramidite is an optically pure isomer (not a mixture). The label is treated as if it were simply another base: It is inserted at the appropriate place in the sequence and incorporated automatically. • Specialty phosphoramidites allow online addition of the label during synthesis, saving time in production of labeled probes and primers • Designed and optimized for use with ABI™ synthesizers, guaranteeing reliable nucleic acid synthesi...
Catalog: 403169
Size: 45 mg
Size: 45 mg
Contains 50 mg TET™ Dye Phosphoramidite. TET™ Dye Phosphoramidite is used for the 5’ attachment of TET™ dye during oligonucleotide synthesis. The label is treated as if it were simply another base: It is inserted at the appropriate place in the sequence and incorporated automatically. • Specialty phosphoramidites allow online addition of the label during synthesis, saving time in production of labeled probes and primers • Designed and optimized for use with ABI™ synthesizers, guaranteeing reliable nucleic acid synthesis • Life Technologies offers a range of specialty phosphoramites and linkers...
Catalog: 403171
Size: 50 mg
Size: 50 mg
RNAlater®-ICE is a novel reagent for transitioning frozen tissue to a state that is easily processed for extraction of high quality RNA. Frozen tissues are simply submerged in RNAlater®-ICE and allowed to thaw overnight at -20°C. Once thawed, the tissues can be processed like fresh tissues using standard RNA isolation procedures. No more laborious grinding of frozen tissue to safeguard the RNA. • Process previously frozen tissues like freshly harvested samples • Thawing tissues in RNAlater®-ICE protects RNA from degradation • No more tissue pulverizing with mortar and pestle and awkward transf...
Catalog: 4427575
Size: 10 bottles
Size: 10 bottles
THE Ambion® RNA Storage Solution is a buffer that provides greater RNA stability than standard 0.1 mM EDTA or TE Buffer. This 1 mM sodium citrate, pH 6.4 +/- 0.2 buffer is provided in one bottle containing 50 mL. The last step in every RNA isolation protocol is to resuspend the purified RNA pellet. After painstakingly isolating the RNA, it is crucial that the pellet be suspended and stored in a safe, RNase-free solution. THE RNA Storage Solution has two features that minimize base hydrolysis of RNA: a low pH, and sodium citrate, which is an efficient chelating agent. THE RNA Storage Solution i...
Catalog: AM7001
Size: 50 ml
Size: 50 ml
Chemical Fragmentation of DNA
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