Chargeswitch Rna Purification Kits

PureLink® RNA Mini Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits

The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. • Rapid, Non-Hazardous RNA purification capabilities • Ready-to-use RNA for many downstream applications Rapid and Convenient Column Purification Procedures The PureLink® RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and t...
Catalog: 12183018A
Size: 50 preps

Illumina® TotalPrep™-96 RNA Amplification Kit

Category: Products & Services > Kits & Assays > RNA Amplification Kits

The Ambion® TotalPrep™ RNA Amplification Kit (patent pending) is a complete system for generating biotinylated, amplified RNA for hybridization with Illumina Sentrix® arrays. The kit includes sufficient reagents for 96 reactions. • Perform amplification from just 50 ng of input RNA • Developed for expression profiling using the Sentrix® BeadChips • Bio-16-UTP now included in optimized NTP mix, reducing setup time and maximizing labeling The TotalPrep™ RNA Amplification Kit incorporates extensive improvements to the in vitro transcription reaction (IVT) over the previous TotalPrep™ Kit. The IVT...
Catalog: 4393543
Size: 96 reactions

PureLink® PCR Purification Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > PCR Clean-Up Kits

PureLink® PCR Purification Kits provide rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR products (Figure 1). The simple PureLink® bind/wash/elute procedure: provides two Binding Buffer options for fragments >100 bp or >300 bp* can be performed in either single column or 96-well plate format takes Two proprietary buffers are supplied to meet the needs of different purification requirements. Use the Binding Buffer for routine purifications of double-stranded DNA fragments from 100 bp to 12 kb. Use Binding Buffer HC for removal of primer...
Catalog: K3100-01
Size: 50 reactions

Illumina® TotalPrep™ RNA Amplification Kit

Category: Products & Services > Kits & Assays > RNA Amplification Kits

The Ambion® Illumina® TotalPrep™ RNA Amplification Kit (patent pending) is a complete system for generating biotinylated, amplified RNA for hybridization with Illumina Sentrix® arrays. The new TotalPrep™ RNA Amplification Kit incorporates extensive improvements to the in vitro transcription reaction (IVT) over the previous Illumina® RNA Amplification Kit. Experience these Improvements: • Improved first-strand cDNA synthesis using ArrayScript™ reverse transcriptase • Biotinylated UTP allows ease of use and optimal labeling • Maximization of cRNA yield using MEGAscript® IVT technology • Signific...
Catalog: AMIL1791
Size: 24 reactions

iPrep™ Trizol® Plus RNA Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits

Use the iPrep™ Trizol® Plus RNA Kit for the highest purity, even from difficult samples such as fatty tissue. This kit provides reproducible RNA sample preparation from up to 50 mg of tissue, 10e7 cultured cells, 10 ml blood, and plant samples. The kit contains pre-filled, fully qualified cartridges to avoid any mix-ups or cross-contamination. It also provides low CVs and reduced purification variability, increasing the reliability of your gene expression assays.
Catalog: IS-10007
Size: 1 kit

MICROB Express ™ Bacterial mRNA Enrichment Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits

This Ambion® kit is for the purification of bacterial mRNA by removing rRNA from total RNA. Sufficient reagents for 20 mRNA purifications, each from 10 µg of total RNA are provided. Features of the MICROB Express ™ Kit: • Dramatically increase the sensitivity of array analyses and other procedures • Removes >95% of 16S and 23S rRNA from bacterial RNA • Simple procedure takes less than 2 hours • Works with many Gram positive and Gram negative bacteria Rapid Purification of Bacterial mRNA is Now Possible Isolating mRNA from bacteria has been virtually impossible, until now. The MICROB Express ™ ...
Catalog: AM1905
Size: 20 reactions

Arcturus® Paradise® Plus RNA Extraction and Isolation

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits

The ARCTURUS® Paradise® PLUS FFPE RNA Isolation Kit permits unprecedented gene expression analysis of millions of previously inaccessible samples by unlocking RNA from formalin-fixed, paraffin-embedded (FFPE) tissues. Amplify as little as 5 ng of the isolated fixed RNA using reagents and methods that have been specially optimized to overcome the challenges of cross-linked FFPE templates (Figure 1). Secure Downstream Success with the Paradise® QC Kit Not all FFPE samples contain high quality RNA. Identify the best samples for your studies using a simple, real-time PCR research technique, and th...
Catalog: KIT0312-I
Size: 12 samples

RNAqueous® Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits

The Ambion® RNAqueous® Kit is for phenol-free total RNA isolation from 0.1 g to 0.5 g of tissue or 10 7 -10 8 cells using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The RNAqueous®-Midi Kit provides enough reagents for 15 RNA purifications. • Convenient syringe-based filtration • Compatible with vacuum manifolds when processing multiple samples The Ambion® RNAqueous® Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through th...
Catalog: AM1912
Size: 50 purifications

MEGAclear™ Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits

The Ambion® MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. MEGAclear™ efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Each kit includes sufficient reagents for 20 reactions. • Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components • High recovery from 1 ng to 500 µg of input • Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections • Quick protocol—purify RNA in le...
Catalog: AM1908M
Size: 20 rxns w⁄ Manual

RediPlate™ 96 RiboGreen® RNA Quantitation Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Quantitation Assay Kits

The RediPlate™ 96 RiboGreen® RNA Quantitation Kit is a rapid, simple, and highly sensitive fluorescence-based RNA-quantitation assay. The microplate has been pre-loaded with RiboGreen® Reagent, which shows bright green fluorescence upon binding to RNA or DNA (1). You simply add buffer and samples to the microplate wells, wait 10 minutes, and then read the fluorescence in any standard fluorescence-based microplate reader. The versatile RediPlate™ 96 design is ideal for either high-throughput quantitation or the measurement of a small number of samples. The RediPlate™ 96 RiboGreen® RNA Quantitat...
Catalog: R32700
Size: 1 kit

Ion Total RNA-Seq Kit for AB Library Builder™ System

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion Total RNA-Seq Kit for AB Library Builder™ System combines optimized reagents and protocols for automated preparation of representative cDNA libraries for strand-specific RNA sequencing of all types of RNA species on the Ion Personal Genome Machine (PGM™) and Proton™ sequencers. Either small RNA (such as microRNA) or whole transcriptome RNA samples can be prepared for next-generation sequencing. The Ion Total RNA-Seq Kit for AB Library Builder™ System contains the same performance advantages as the manual Ion Total RNA-Seq Kit v2 with the convenience of protocol automation. The gel size...
Catalog: 4482416
Size: 13 reactions

Ion AmpliSeq™ RNA Library Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.lifetechnologies.com/confirmrna . Key Features: • Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels • Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA • Ion Ampl...
Catalog: 4482335
Size: 8 reactions

Ion AmpliSeq™ RNA Library Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.lifetechnologies.com/confirmrna . Key Features: • Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels • Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA • Ion Ampl...
Catalog: 4482340
Size: 96 reactions

Ion AmpliSeq™ RNA Library Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.lifetechnologies.com/confirmrna . Key Features: • Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels • Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA • Ion Ampl...
Catalog: 4482752
Size: 384 reactions

5500 W REV1 (RNA) SP Kit

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Sequencing Primers

The 5500 W REV1 (RNA) SP Kit contains sequencing primers and probes for whole transcriptome libraries in paired-end sequencing runs. This kit is used to prime the F5 tag for ligation sequencing on the 5500 W FlowChip V2 for RNA libraries. Each kit includes one strip tube containing sufficient specific sequencing primers and bridge probes to run 6 lanes.
Catalog: 4475683
Size: 1 kit

PureLink® Viral RNA/DNA Mini Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Viral RNA⁄DNA Kits

The PureLink® Viral RNA/DNA Mini Kit is a nucleic acid purification system designed for fast and easy isolation of viral RNA or DNA from cell-free samples, such as serum, plasma, and cerebrospinal fluid. Starting sample volumes can be as high as 500 µl and elution volumes as low as 10 µl, allowing for a 50-fold concentration of nucleic acids. The higher target concentration obtained using the PureLink® Viral RNA/ DNA Mini Kit provides greater sensitivity and accuracy in downstream detection procedures such as real-time PCR or endpoint analysis. Specially formulated buffers enable the purificat...
Catalog: 12280-050
Size: 50 preps