The PureLink® HiPure Plasmid Purification Systems are designed to isolate transfection-grade plasmid DNA from E. coli in less than 2 hours. The Maxiprep Kit protocol will typically yield 500–850 µg plasmid DNA from 100–200 ml bacterial culture, at a purity that is comparable to that achieved with two passes through cesium chloride gradients. Advantages of the PureLink® HiPure Plasmid Maxiprep Kit include: • Labor- and time-saving —No need for extra steps to remove endotoxin and other contaminants • High purity, low endotoxin product —pure enough for mammalian cell transfection • Versatility —p...
Size: 25 preps
Invitrogen makes blotting of E-PAGE™ Gels easier and more efficient by providing a pre-cut fiber blotting pad and several types of pre-cut, pre-assembled membrane/filter paper sandwiches that perfectly fit 7.5 cm x 8.4 cm E-PAGE™ Gels*. The available nitrocellulose and Invitrolon™ PVDF membranes are designed for western transfer of proteins (Figure 1, Table 1). Pre-cut, extra-thick, absorbent Blotting Paper (2.5 mm thickness) for E-PAGE" Gels are also available to accommodate the E-PAGE™ blotting set-up using a semi-dry transfer apparatus. A protein s properties affect its ability to bind to m...
Size: 50 sheets
Fast, simple protocols - Purify your plasmid DNA in less than 2 hours Labor-saving - No need for extra steps to remove endotoxin and other contaminants Megapreps and Gigapreps save time by utilizing vacuum-assisted cartridges PureLink® HiPure Plasmid Purification Systems are designed to isolate plasmid DNA of the highest purity (1) with a wide scale range (Table 1). Simple protocols an anion-exchange resin allow you to purify plasmid DNA (Figure 1) at a quality equivalent to two passes through cesium chloride gradients - the most rigorous method for DNA purification. In less than 2 hours, DNA ...
Size: 4 preps
The Vybrant Apoptosis Assay Kit #4 detects apoptosis on the basis of changes that occur in the permeability of cell membranes. This kit contains ready-to-use solutions of both YO-PRO-1 and propidium iodide nucleic acid stains. YO-PRO-1 stain selectively passes through the plasma membranes of apoptotic cells and labels them with moderate green fluorescence. Necrotic cells are stained red-fluorescent with propidium iodide.
Size: 1 kit
Pro-Q Diamond phosphoprotein gel stain provides a convenient method for selectively staining phosphoproteins in acrylamide gels, without the need for blotting or phosphoprotein specific antibodies and Western blot analysis. Preparation of the Pro-Q Diamond phosphoprotein gel destaining solution is described in the gel staining protocol, but we also provide the detaining solution premixed for your convenience.
Size: 1 L
Molecular Probes' proprietary Pro-Q Emerald 300 and Pro-Q Emerald 488 Glycoprotein Stain Kits provide the most advanced technology available for detection of glycoproteins in gels and on blots. Gel staining is rapid and very sensitive. In less than three hours, it is possible to detect as little as 300 pg of glycoprotein per band, depending on the degree of glycosylation, making these stains at least 50-fold more sensitive than standard fuchsin staining.
Size: 1 kit
The XCell line of mini-cells has always distinguished itself by being simple, sturdy, and convenient. The tradition continues with the patented XCell SureLock ® Mini-Cell. The incorporation of a gel tension wedge in place of the rear wedge used on earlier models makes the XCell SureLock ® the easiest-to-use mini-cell on the market. How the System Works The XCell SureLock ® Mini-Cell holds gels firmly with a simple gel tension wedge. When the lever on the gel tension wedge is pushed forward into the locked position, an even horizontal force is generated. This seals the gel/buffer core assembly ...
Size: 1 unit
The TaqMan® GTXpress™ Master Mix delivers accurate genotyping results with robust performance in less than 50 min. Applied Biosystems® TaqMan® GTXpress™ Master Mix (2X) combines AmpliTaq® Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format. The 1 mL pack includes reagents sufficient to perform 400 PCR reactions (5 µL/reaction). • Compatible with both Fast and Standard mode thermal cycling conditions • Pre-mixed components stored at 2°–8°C significantly reduce assay setup time • Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays •...
Size: 400 reactions
The Mini PAP pen draws a water repellent circle around slide mounted tissue, preventing the waste of valuable reagents by keeping the liquid pooled in a single droplet. The Mini PAP Pen is especially useful when working with multiple sections on a single slide because its tip is smaller than the PAP pen. Each pen can be used to draw about 400 circles.
Size: 1 pen
The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. • Rapid, Non-Hazardous RNA purification capabilities • Ready-to-use RNA for many downstream applications Rapid and Convenient Column Purification Procedures The PureLink® RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and t...
Size: 50 preps
DryEase® Mini-Gel Cellophane is one component of the DryEase® Mini-Gel Drying System , which is a simple, easy-to-use kit for drying gels evenly using passive evaporation. The kit allows gels to be assembled on a base between two open frames, so that both outer surfaces of the gels are exposed to air. Consequently, drying is even and cracking is minimized. The proprietary Gel-Dry™ Drying Solution modulates the rate of water release to ensure crack-free gels. With the DryEase® System, gels are dried overnight with uniform tension on all points of the gel to eliminate cracking. Applications: Gel...
Invitrogen makes blotting of E-PAGE™ Gels easier and more efficient by providing a pre-cut fiber blotting pad and several types of pre-cut, pre-assembled membrane/filter paper sandwiches that perfectly fit 8.6 cm x 13.5 cm E-PAGE™ Gels*. The available nitrocellulose and Invitrolon™ PVDF membranes are designed for western transfer of proteins (Figure 1, Table 1). Pre-cut, extra-thick, absorbent Blotting Paper (2.5 mm thickness) for E-PAGE" Gels are also available to accommodate the E-PAGE™ blotting set-up using a semi-dry transfer apparatus. A protein s properties affect its ability to bind to ...
Zero Blunt® TOPO® PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR®-Blunt II-TOPO® Vector for 5-minute benchtop ligations without ligase (1). The pCR®-Blunt II-TOPO® Vector also features: • ccdB gene for positive selection (2) • Eco R I sites flanking the PCR product insertion site for easy excision of inserts • Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli • SP6 promoter/primer site for...
Size: 20 preps
The PureLink® Viral RNA/DNA Mini Kit is a nucleic acid purification system designed for fast and easy isolation of viral RNA or DNA from cell-free samples, such as serum, plasma, and cerebrospinal fluid. Starting sample volumes can be as high as 500 µl and elution volumes as low as 10 µl, allowing for a 50-fold concentration of nucleic acids. The higher target concentration obtained using the PureLink® Viral RNA/ DNA Mini Kit provides greater sensitivity and accuracy in downstream detection procedures such as real-time PCR or endpoint analysis. Specially formulated buffers enable the purificat...
Size: 50 preps
This product contains three boxes of 10 sets/box of iBlot® Transfer Stack, Mini, Nitrocellulose (Cat# IB3010-02) The iBlot® Dry Blotting System efficiently and reliably transfers proteins from polyacrylamide gels in seven minutes or less without the need for buffers or an external power supply. A self-contained unit (Figure 1), the iBlot® device uses disposable blotting stacks with integrated transfer membranes (nitrocellulose and PVDF), offering the convenience of a bufferless, plug-and-play system. Compared to conventional blotting techniques, the iBlot® system enables higher downstream dete...
Size: 3 x 10 sets⁄box
Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR® Green RNA-to-C T ™ 1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes: • AmpliTaq® Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation • ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA • An additive that red...
Size: 40 reactions
TaqMan® Universal Master Mix II is a convenient 2X mix for use in all real-time application including microRNA, gene expression and genotyping. TaqMan® Universal Master Mix II is a convenient 2X mix that includes: -AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure), a highly purified thermostable DNA polymerase, that is hot start-enabled for convenient reaction setup at room temperature -Available in two formats: with and without uracil-DNA glycosylase (UDG) and dUTP⁄dTTP mix to minimize carryover PCR contamination -Optimized salt, dNTP, and buffer concentrations for reliable performance -Passive ...
Size: 40 reactions
Size: 1 each
The Bolt® Mini Gel Tank is compatible with Bolt® Bis-Tris Plus gels, and can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt® Bis-Tris Plus gels to be run in approximately 35 minutes. Run times may vary depending on buffer conditions and power supplies being used. Each Bolt® Mini Gel Tank includes: • Gel Runner Tank • Bolt® Tank Base • Bolt® Tank Lid • Cassette Clamps, Right and Left • Novex® Power Supply Adapters • Gel Knife About Bolt® Bis-Tris Pl...
Size: 1 unit