Size: 100 tablets
Size: 12 × 1 L
Molecular biology grade, Ambion® PBS 10X, pH 7.4 solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminatin...
Size: 1 L
Molecular biology grade, Ambion® PBS 10X, pH 7.4 solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contamina...
Size: 500 ml
Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Life Technologies offers a variety of Gibco® PBS formulations for a range of cell culture applications. This PBS is manufactured as follows: Without • Calcium • Magnesium • Phenol Red
Size: 500 ml
Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Life Technologies offers a variety of Gibco® PBS formulations for a range of cell culture applications. Find the right formulation using the media selector tool . This PBS is manufactured as follows: Without • Calcium • Magnesium • Phenol Red
Size: 10 x 500 ml
The same lysis buffer found in the Ambion® Cells-to-Signal™ Kit. Supplied in 5 bottles containing 10 mL each.
Size: 5 bottle
Cells-to-C T Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription in the TaqMan® Gene Expression Cells-to-C T ™ Kit (20X RT enzyme mix and 20X RT buffer). These reagents are sufficient for 2500 RT reactions. When combined with the Cells-to- C T Bulk Lysis Reagents , these reagents can be easily incorporated into automated, high throughput applications.
Size: 2500 reactions
Gibco® Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS). Gibco® Cell Dissociation Buffer is suitable for the gentle dissociation of mammalian cells for studies such as ligand binding, flow cytometry, and immunohistochemistry, that require intact cell surface proteins. Gibco® Cell Dissociation Buffer works well with lightly adherent cell lines, such as HeLa and NIH 3T3, but is not recommended for the routine passaging of strongly adherent...
Size: 100 ml
Cell extraction buffer suitable for use in ELISA and Western blotting.
Size: 100 ml
NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay (Luminex), ELISA, and Western blotting.
Size: 100 ml
These 2 buffers are to be used only as a complement to the SuperScript® III Platinum® CellsDirect Kits when large number of samples are processed. See manual of SuperScript® III Platinum® CellsDirect Kits for protocol recommendations.
Size: 1 kit
Gibco® ACK (Ammonium-Chloride-Potassium) Lysing Buffer is suitable for use in the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats and bone marrow. The complete formulation and lysis protocol are available in our Technical Reference Library. cGMP Manufacturing and Quality System Gibco® ACK Lysing Buffer is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards.
Size: 100 ml
Total Exosome Isolation (from cell culture media) enables fast and efficient enrichment of intact exosomes from cell culture media samples. • Maximize recovery of intact exosomes for any type of downstream application • Easily isolate exosomes using our simple and reliable protocol • Avoid time-consuming ultra-centrifugation • Maximize flexibility—can be scaled up or down depending on the sample size Isolation of exosomes can be a tedious, non-specific, and difficult process. Total Exosome Isolation (from cell culture media) enables simple and reliable concentration of intact exosomes from cel...
Size: 50 ml
Trypan blue stain is a well-accepted method for staining dead cells. It can enter into cells with compromised membranes and is the method used with the Countess® automated cell counting platform.
Size: 2 × 1 ml
StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. Accutase® does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex™, CELLStart™ and polymers. Accutase® performs exceptionally well in detaching primary and stem cells and maintaining high cell viabilty compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase® treatment include analysis of cell su...
Size: 100 ml
Invitrogen's Calcium Phosphate Transfection Kit provides high-quality reagents to enable the introduction of DNA into eukaryotic cells via calcium phosphate co-precipitation.
Size: 75 reactions
StemPro® CD34+ cells are pooled Human Hematopoietic Progenitor cells (HPCs) derived from the umbilical cord blood of mixed donors. Along with 0.5×10 6 pooled donor cord blood-derived CD34+ cells, this kit includes StemPro®-34 SFM basal liquid medium and frozen StemPro®-34 Nutrient Supplement to facilitate the immediate culturing of the StemPro® CD34+ cells upon arrival or when recovering from cryopreservation storage. With the StemPro® CD34+ Cell Kit You Get: • ≥90% purity of the desired CD34+ cell population • Each vial of cells contains 0.5×10 6 CD34+ cells with full traceability • StemPro®-...
Size: 0.5 x 10
Mouse Embryonic stem cells (ESCs) were isolated from mouse (Strain C57BL⁄6) embryos at Day E3.5. Before cryopreservation, the cells were tested for viability (>70% post-thaw viability), their ability to remain undifferentiated when cultured on mouse feeder cells as assayed by marker expression (Oct4, SSEA-1, Nanog) and their ability to differentiate into embryoid bodies and express markers indicative of three germ layers (Ectoderm: (+) for β3-tubulin and nestin, Mesoderm: (+) for smooth muscle actin, Endoderm: (+) for AFP). Cells were cryopreserved at passage 20 (P20). For optimal growth perfo...
Size: 1 ml
The Violet Ratiometric Membrane Asymmetry Probe⁄Dead Cell Apoptosis Kit provides a simple and fast method for the detection of apoptosis with dead cell discrimination by flow cytometry. The Violet Ratiometric Membrane Asymmetry Probe, 4'-N,N-diethylamino-6-(N,N,N-dodecyl-methylamino-sulfopropyl)-methyl-3-hydroxyflavone (F2N12S), is a novel violet excitable dye for the detection of membrane asymmetry changes during apoptosis. The dye exhibits an excited-state intramolecular proton transfer (ESIPT) reaction resulting in a dual fluorescence with two emission bands corresponding to 530 nm and 585 ...
Size: 1 kit