Cells-to-Ct Cell-Free Expression Kits
Gibco® Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS). Gibco® Cell Dissociation Buffer is suitable for the gentle dissociation of mammalian cells for studies such as ligand binding, flow cytometry, and immunohistochemistry, that require intact cell surface proteins. Gibco® Cell Dissociation Buffer works well with lightly adherent cell lines, such as HeLa and NIH 3T3, but is not recommended for the routine passaging of strongly adherent...
Catalog: 13151-014
Size: 100 ml
Size: 100 ml
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Catalog: K101-01
Size: 20 reactions
Size: 20 reactions
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Catalog: K100-01
Size: 20 reactions
Size: 20 reactions
Cells-to-C T Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription in the TaqMan® Gene Expression Cells-to-C T ™ Kit (20X RT enzyme mix and 20X RT buffer). These reagents are sufficient for 2500 RT reactions. When combined with the Cells-to- C T Bulk Lysis Reagents , these reagents can be easily incorporated into automated, high throughput applications.
Catalog: 4391852C
Size: 2500 reactions
Size: 2500 reactions
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein. In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to...
Catalog: K6025-01
Size: 20 reactions
Size: 20 reactions
The pcDNA™3.1/V5-His TOPO® TA Expression Kit offers one-step cloning of Taq -amplified PCR products into a high-level expression vector. Topoisomerase activation of the pcDNA3.1/V5-His-TOPO® vector allows PCR products to be ligated in just 5 minutes on your bench top and results in 90% recombinants. In addition, the vector includes the following features: Strong CMV promoter for high-level, constitutive expression. C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody. C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating...
Catalog: K4800-40
Size: 40 reactions
Size: 40 reactions
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 12562-013
Size: 5 transfections
Size: 5 transfections
The WT Expression Kit is an RNA amplification innovation from Ambion® that is designed to generate amplified sense-strand cDNA ready for fragmentation and labeling using the Affymetrix® GeneChip® WT Terminal Labeling Kit. Features of the Ambion® WT Expression Kit: • Optimized for use with Affymetrix® GeneChip® Human, Mouse, and Rat 1.0 ST Arrays • Allows RNA samples as small as 50 ng to be analyzed on Affymetrix® GeneChip® Arrays • Supports a streamlined workflow that does not require a separate rRNA depletion step • Is ideal for high-sensitivity expression profiling Eliminates Separate rRNA D...
Catalog: 4411974
Size: 30 reactions
Size: 30 reactions
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein. In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to...
Catalog: K4202-01
Size: 20 reactions
Size: 20 reactions
The pcDNA™3.1 Directional TOPO® Expression Kit uses linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO® for five-minute directional cloning and subsequent high-level expression. Directional Cloning technology facilitates expression experiments because: A proofreading enzyme is used for fewer errors in cloned genes Greater than 90% of the clones are in the correct orientation for expression, reducing time spent colony screening for clone orientation In addition, pcDNA3.1D/V5-His-TOPO® provides strong expression levels from the CMV promoter and the option of a C-terminal V5-His fusion t...
Catalog: K4900-40
Size: 40 reactions
Size: 40 reactions
The DES® TOPO® TA Expression Kit offers one-step cloning of PCR products directly into an inducible DES® expression vector. The kit uses the linearized, topoisomerase I-activated pMT/V5-His-TOPO® vector for fast and easy cloning and subsequent high-level expression. Topoisomerase activation of this vector allows PCR products to be ligated in just 5 minutes on your bench top to produce >85%recombinants. The pMT/V5-His-TOPO® vector has all of the features of the pMT/V5-His vector to simplify protein expression, detection, and purification of recombinant proteins in Drosophila S2 cells.
Catalog: K4125-01
Size: 20 reactions
Size: 20 reactions
The ViraPower™ HiPerform™ Lentiviral FastTiter™ TOPO® Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral and TOPO® technologies to facilitate quick PCR-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti7.3/V5-TOPO® vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central ...
Catalog: K5320-00
Size: 1 kit
Size: 1 kit
The ViraPower™ Lentiviral Directional TOPO® Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. It combines Invitrogen’s ViraPower™ Lentiviral and Directional TOPO® technologies to facilitate quick PCR-based cloning and lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6⁄V5-D-TOPO® vector has the CMV promoter for driving constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells. Advantages • Lentivirus...
Catalog: K4950-00
Size: 1 kit
Size: 1 kit
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/GW/D-TOPO vector offers the following key features: •Cytomegalovirus (CMV) promoter for high-level expression •Adapted for Directional TOPO® Cloning, enabling you to use proofreading polymerases and to clone your PCR products in a specific orientation •Neomycin resistance gene for stable selection •C-terminal V5 tag for easy detection •Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli TOPO® Cloning Using restriction enzymes to clone your ge...
Catalog: K2440-20
Size: 20 reactions
Size: 20 reactions
The GlobalFiler™ Express Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. Only Life Technologies can offer the GlobalFiler™ Express Kit as part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for database and single-source samples; for casework samples, p...
Catalog: 4474665
Size: 1000 reactions
Size: 1000 reactions
The GlobalFiler™ Express Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. Only Life Technologies can offer the GlobalFiler™ Express Kit as part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for database and single-source samples; for casework samples, p...
Catalog: 4476609
Size: 200 reactions
Size: 200 reactions
Novex® Tris-Glycine Express Kits are a convenient and cost-effective way to obtain small quantities of pre-cast tris-glycine gels and pre-stained protein standard. Each kit includes: • 5 Novex® 4-20% Tris-Glycine Mini Gels, 1.0 mm, 12 well • 50 µl of SeeBlue® Pre-Stained Standard Novex® Tris-Glycine Mini Gels Novex® Tris-Glycine Mini Gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. • Individually packaged gels for optimal experiment design • Compatibl...
Catalog: EC60252BX5
Size: 5 gels
Size: 5 gels
StemPro® CD34+ cells are pooled Human Hematopoietic Progenitor cells (HPCs) derived from the umbilical cord blood of mixed donors. Along with 0.5×10 6 pooled donor cord blood-derived CD34+ cells, this kit includes StemPro®-34 SFM basal liquid medium and frozen StemPro®-34 Nutrient Supplement to facilitate the immediate culturing of the StemPro® CD34+ cells upon arrival or when recovering from cryopreservation storage. With the StemPro® CD34+ Cell Kit You Get: • ≥90% purity of the desired CD34+ cell population • Each vial of cells contains 0.5×10 6 CD34+ cells with full traceability • StemPro®-...
Catalog: A14059
Size: 0.5 x 10
Size: 0.5 x 10
Trypan blue stain is a well-accepted method for staining dead cells. It can enter into cells with compromised membranes and is the method used with the Countess® automated cell counting platform.
Catalog: T10282
Size: 2 × 1 ml
Size: 2 × 1 ml
Mouse Embryonic stem cells (ESCs) were isolated from mouse (Strain C57BL⁄6) embryos at Day E3.5. Before cryopreservation, the cells were tested for viability (>70% post-thaw viability), their ability to remain undifferentiated when cultured on mouse feeder cells as assayed by marker expression (Oct4, SSEA-1, Nanog) and their ability to differentiate into embryoid bodies and express markers indicative of three germ layers (Ectoderm: (+) for β3-tubulin and nestin, Mesoderm: (+) for smooth muscle actin, Endoderm: (+) for AFP). Cells were cryopreserved at passage 20 (P20). For optimal growth perfo...
Catalog: S1503-100
Size: 1 ml
Size: 1 ml
Cells-to-Ct Cell-Free Expression Kits
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