Cells-to-Ct Balanced Salt Solutions
Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Life Technologies offers a variety of Gibco® PBS formulations for a range of cell culture applications. Find the right formulation using the media selector tool . This PBS is manufactured as follows: Without • Calcium • Magnesium • Phenol Red
Catalog: 10010-023
Size: 500 ml
Size: 500 ml
Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Life Technologies offers a variety of Gibco® PBS formulations for a range of cell culture applications. Find the right formulation using the media selector tool . This PBS is manufactured as follows: Without • Calcium • Magnesium • Phenol Red
Catalog: 20012-068
Size: 10 x 500 ml
Size: 10 x 500 ml
Cells-to-C T Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription in the TaqMan® Gene Expression Cells-to-C T ™ Kit (20X RT enzyme mix and 20X RT buffer). These reagents are sufficient for 2500 RT reactions. When combined with the Cells-to- C T Bulk Lysis Reagents , these reagents can be easily incorporated into automated, high throughput applications.
Catalog: 4391852C
Size: 2500 reactions
Size: 2500 reactions
The Vybrant® DiD cell-labeling solution is a dye delivery solution that can be added directly to normal culture media to uniformly label suspended or attached culture cells for use in cell-cell fusion, cellular adhesion and migration applications.
Catalog: V-22887
Size: 1 ml
Size: 1 ml
DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a long-term tracer for neuronal and other cells. DiI is also available as a solid (D-282), as a paste (N-22880) or as crystals (D-3911).
Catalog: V-22885
Size: 1 ml
Size: 1 ml
The Vybrant® CM-DiI cell-labeling solution is a dye delivery solution that can be added directly to normal culture media to uniformly label suspended or attached culture cells for use in cell-cell fusion, cellular adhesion and migration applications.
Catalog: V-22888
Size: 1 ml
Size: 1 ml
Gibco® MEM Vitamin Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Vitamin Solution is formulated to contain 100x the vitamins found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Vitamin Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001...
Catalog: 11120-052
Size: 100 ml
Size: 100 ml
Gibco® MEM Amino Acids Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Amino Acids Solution is formulated to contain 50X the essential amino acids (except L-glutamine) found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Amino Acids Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufactur...
Catalog: 11130-051
Size: 100 ml
Size: 100 ml
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca 2+ . Fluo-3 has been used to image the spatial dynamics of Ca 2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt ...
Catalog: F-14200
Size: 500 µg
Size: 500 µg
Fura-FF pentapotassium salt is a fura-2 derivative that has Ca 2+ sensitivity similar to mag-fura-2, but is relatively insensitive to Mg 2+ . This water-soluble salt form is useful for intracellular loading by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent (I-14402).
Catalog: F14180
Size: 500 µg
Size: 500 µg
Trypan blue stain is a well-accepted method for staining dead cells. It can enter into cells with compromised membranes and is the method used with the Countess® automated cell counting platform.
Catalog: T10282
Size: 2 × 1 ml
Size: 2 × 1 ml
Hygromycin B is a water-soluble antibiotic purified from the bacterium Streptomyces hydroscopicus . Hygromycin B acts by binding to the 80S subunit of the bacterial ribosome and inhibiting protein synthesis. Gibco® Hygromycin B is used as a bacterial selective antibiotic in the concentration range of 100 - 1000 µg / ml. This product is supplied as a 50 mg / ml solution. Resistance to Hygromycin B is conferred by the E. coli hygromycin resistance gene ( hyg or hph ). Hygromycin B uses a different mode of action than Geneticin®, Blasticidin S, or Zeocin™, so it is excellent for use in dual-selec...
Catalog: 10687-010
Size: 20 ml
Size: 20 ml
StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. Accutase® does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex™, CELLStart™ and polymers. Accutase® performs exceptionally well in detaching primary and stem cells and maintaining high cell viabilty compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase® treatment include analysis of cell su...
Catalog: A11105-01
Size: 100 ml
Size: 100 ml
Geneticin® Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin® Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 - 200 µg / ml for bacteria, or 200 – 500 µg / ml for most mammalian cells. This product is supplied as a 50 mg / ml solution in...
Catalog: 10131-027
Size: 100 ml
Size: 100 ml
StemPro® CD34+ cells are pooled Human Hematopoietic Progenitor cells (HPCs) derived from the umbilical cord blood of mixed donors. Along with 0.5×10 6 pooled donor cord blood-derived CD34+ cells, this kit includes StemPro®-34 SFM basal liquid medium and frozen StemPro®-34 Nutrient Supplement to facilitate the immediate culturing of the StemPro® CD34+ cells upon arrival or when recovering from cryopreservation storage. With the StemPro® CD34+ Cell Kit You Get: • ≥90% purity of the desired CD34+ cell population • Each vial of cells contains 0.5×10 6 CD34+ cells with full traceability • StemPro®-...
Catalog: A14059
Size: 0.5 x 10
Size: 0.5 x 10
Mouse Embryonic stem cells (ESCs) were isolated from mouse (Strain C57BL⁄6) embryos at Day E3.5. Before cryopreservation, the cells were tested for viability (>70% post-thaw viability), their ability to remain undifferentiated when cultured on mouse feeder cells as assayed by marker expression (Oct4, SSEA-1, Nanog) and their ability to differentiate into embryoid bodies and express markers indicative of three germ layers (Ectoderm: (+) for β3-tubulin and nestin, Mesoderm: (+) for smooth muscle actin, Endoderm: (+) for AFP). Cells were cryopreserved at passage 20 (P20). For optimal growth perfo...
Catalog: S1503-100
Size: 1 ml
Size: 1 ml
The Violet Ratiometric Membrane Asymmetry Probe⁄Dead Cell Apoptosis Kit provides a simple and fast method for the detection of apoptosis with dead cell discrimination by flow cytometry. The Violet Ratiometric Membrane Asymmetry Probe, 4'-N,N-diethylamino-6-(N,N,N-dodecyl-methylamino-sulfopropyl)-methyl-3-hydroxyflavone (F2N12S), is a novel violet excitable dye for the detection of membrane asymmetry changes during apoptosis. The dye exhibits an excited-state intramolecular proton transfer (ESIPT) reaction resulting in a dual fluorescence with two emission bands corresponding to 530 nm and 585 ...
Catalog: A-35137
Size: 1 kit
Size: 1 kit
Geneticin® Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin® Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 - 200 µg / ml for bacteria, or 200 – 500 µg / ml for most mammalian cells. This product is supplied as a 50 mg / ml solution in...
Catalog: 10131-019
Size: 20 ml
Size: 20 ml
Sodium Pyruvate is commonly added to cell culture medium as an energy source in addition to glucose. Since the cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not necessary to supplement all cultures. However, if the cells have been grown in medium supplemented with sodium pyruvate, we recommend the supplementation is continued as the cells become accustomed to this addition and their growth will lag without it. Gibco® Sodium Pyruvate, 100 mM Solution is formulated as 11 g per liter of water. The final concentration of sodium pyruvate used in most cell...
Catalog: 11360-070
Size: 100 ml
Size: 100 ml
Cells-to-Ct Balanced Salt Solutions
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