Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents
Total Exosome Isolation (from cell culture media) enables fast and efficient enrichment of intact exosomes from cell culture media samples. • Maximize recovery of intact exosomes for any type of downstream application • Easily isolate exosomes using our simple and reliable protocol • Avoid time-consuming ultra-centrifugation • Maximize flexibility—can be scaled up or down depending on the sample size Isolation of exosomes can be a tedious, non-specific, and difficult process. Total Exosome Isolation (from cell culture media) enables simple and reliable concentration of intact exosomes from cel...
Catalog: 4478359
Size: 50 ml
Category: Products & Services > Beads & Microspheres > Beads for Organelle Isolation
Exosome – Human CD63 Isolation/Detection enables the purification of CD63+ exosomes (also called extracellular vesicles and multi-vesicular bodies) from pre-enriched cell culture samples. These exosomes can then be detected using techniques such as flow cytometry, electron microscopy, or Western blotting. The exosomes must be pre-enriched before isolation. This can be done by ultracentrifugation, or by using the fast and efficient Total Exosome Isolation (from cell culture media) reagent. • Obtain highly pure CD63+ exosomes • "See" your sample during handling • Easily scalable protocol • Detec...
Catalog: 106-06D
Size: 3 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media
Formulation per one liter: 10 g SELECT Peptone 140 5 g SELECT Yeast Extract 5 g sodium chloride
Catalog: 12780-029
Size: 2.5 kg
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media
Application(s): For growth of hosts for replication of M13 vectors. Instructions for use: 1. Suspend 31 g in 1 L of demineralized water. 2. Autoclave for 15 min at 121°C.
Catalog: 22712-020
Size: 500 g
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > McCoy's 5A
McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues. This McCoy's 5A is modified as follows: With Without • High Glucose • Sodium Pyruvate • L-glutamine • HEPES • Bacto-peptone • Phenol Red
Catalog: 16600-082
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media
imMedia™ growth medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia™ growth medium contains everything you need—including your choice of antibiotics and optional X-gal/IPTG—to prepare low-salt LB medium or agar plates. You'll save hours of medium preparation time because there is: • No weighing or mixing of media components • No autoclaving • No waiting for media to cool before adding antibiotics Fast Medium Preparation imMedia™ growth medium is fast food for E. coli . Simply mix with water in a clean flask, heat in a...
Catalog: Q601-20
Size: 20 each
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Iscove's (IMDM)
Iscove's Modified Dulbecco's Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. Life Technologies offers a variety of Gibco® IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This IMDM is modified as follows: With Without • L-glutamine • Phenol Red • α-thioglycerol • 2-mercaptoethanol
Catalog: 21056-023
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM⁄F-12 Media
Dulbecco's Modified Eagle Medium : Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of Gibco® DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM / F-12 is modified as follows: With Without • L-glutamine • HEPES • Phenol Red
Catalog: 11320-082
Size: 10 × 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • Sodium Pyruvate • L-glutamine • HEPES • Phenol Red
Catalog: 11965-126
Size: 6 × 1000 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media
Formulation per one liter: 10 g SELECT Peptone 140 5 g SELECT Yeast Extract 10 g sodium chloride
Catalog: 12795-027
Size: 500 g
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > 293 Media
FreeStyle™ 293 Expression Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth and transfection of 293-F cells in suspension without adaptation. The medium is complete and ready-to-use, with no supplementation required. Gibco® FreeStyle™ 293 Expression Medium features: • Complete and ready-to-use format containing GlutaMAX™ supplement • Ability to support suspension growth and transfection of 293-F cells • Animal origin-free, protein-free and chemically defined formulation • Easy use as part of the FreeStyle™ 293 ...
Catalog: 12338-018
Size: 1000 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Minimum Essential Media (MEM)
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • NEAA • L-glutamine • Phenol Red • HEPES
Catalog: 10370-088
Size: 10 × 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM⁄F-12 Media
Dulbecco's Modified Eagle Medium : Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of Gibco® DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM / F-12 is modified as follows: With • L-glutamine • HEPES • Phenol Red
Catalog: 11330-032
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Minimum Essential Media (MEM)
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • HEPES • L-glutamine • Phenol Red
Catalog: 12360-038
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Opti-MEM® I
Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications. This Opti-MEM® is modified ...
Catalog: 31985-070
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES • L-methio...
Catalog: 21013-024
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Virus Production Media
VP-SFM AGT™ is a serum free, ultra low protein medium (5 µg/ml) in a new easy-to-use granular format. VP-SFM AGT™ is designed for the growth of VERO cells for virus production. It is also suitable for the growth of COS-7, MDCK, BHK-21 and Hep2 cells and for recombinant protein production.
Catalog: 12559-019
Size: 10 L
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • Low Glucose • HEPES • Sodium Pyruvate • GlutaMAX™ • Phenol Red
Catalog: 10567-014
Size: 500 ml