Cell extraction buffer suitable for use in ELISA and Western blotting.
Size: 100 ml
NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay (Luminex), ELISA, and Western blotting.
Size: 100 ml
The same lysis buffer found in the Ambion® Cells-to-Signal™ Kit. Supplied in 5 bottles containing 10 mL each.
Size: 5 bottle
These 2 buffers are to be used only as a complement to the SuperScript® III Platinum® CellsDirect Kits when large number of samples are processed. See manual of SuperScript® III Platinum® CellsDirect Kits for protocol recommendations.
Size: 1 kit
Gibco® Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Phosphate Buffered Saline (PBS). Gibco® Cell Dissociation Buffer is suitable for gentle dissociation of mammalian cells for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry. Gibco® Cell Dissociation Buffer works well with lightly adherent cell lines such as HeLa and NIH 3T3, but is not recommended for routine passage of strongly adherent cells. The dis...
Size: 100 ml
The XCell line of mini-cells has always distinguished itself by being simple, sturdy, and convenient. The tradition continues with the patented XCell SureLock ® Mini-Cell. The incorporation of a gel tension wedge in place of the rear wedge used on earlier models makes the XCell SureLock ® the easiest-to-use mini-cell on the market. How the System Works The XCell SureLock ® Mini-Cell holds gels firmly with a simple gel tension wedge. When the lever on the gel tension wedge is pushed forward into the locked position, an even horizontal force is generated. This seals the gel/buffer core assembly ...
Size: 1 unit
Gibco® ACK (Ammonium-Chloride-Potassium) Lysing Buffer is suitable for use in the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats and bone marrow. The complete formulation and lysis protocol are available in our Technical Reference Library. cGMP Manufacturing and Quality System Gibco® ACK Lysing Buffer is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards.
Size: 100 ml
The Protein Quant Sample Lysis Kit contains the reagents required for the preparation of target and control cell lysates for use with TaqMan® Protein Assay experiments. It contains a one-step lysis solution for preparing crude cell lysates from cultured cells, and the lysates are compatible with cell counting as well as total protein quant measurements such as a microBCA assay. The Protein Quant Sample Lysis Kit contains a buffered nonionic detergent solution for the gentle lysis of adherent and non-adherent cultured cells under non-denaturing conditions
Size: 25 ml
Total Exosome Isolation (from cell culture media) enables fast and efficient enrichment of intact exosomes from cell culture media samples. • Maximize recovery of intact exosomes for any type of downstream application • Easily isolate exosomes using our simple and reliable protocol • Avoid time-consuming ultra-centrifugation • Maximize flexibility—can be scaled up or down depending on the sample size Isolation of exosomes can be a tedious, non-specific, and difficult process. Total Exosome Isolation (from cell culture media) enables simple and reliable concentration of intact exosomes from cel...
Size: 50 ml
Cells-to-C T Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription in the TaqMan® Gene Expression Cells-to-C T ™ Kit (20X RT enzyme mix and 20X RT buffer). These reagents are sufficient for 2500 RT reactions. When combined with the Cells-to- C T Bulk Lysis Reagents , these reagents can be easily incorporated into automated, high throughput applications.
Size: 2500 reactions
The MagicMark™ XP Western Protein Standard is specifically designed for easy and convenient protein molecular weight estimation directly on western blots. The standard consists of 9 recombinant proteins each of which contains an IgG binding site. The IgG binding site binds the primary or secondary antibody used for detection of the target protein allowing direct visualization of the standard on the western blot. The MagicMark™ XP Standard is compatible with all western kits and substrates (chemiluminescent, chromogenic and fluorescent) and offers: • Direct visualization of molecular weight sta...
Size: 250 µl
Invitrogen Cal-Lyse™ is a lysing solution formulated to enable the lysis of erythrocytes in samples of anticoagulated human peripheral blood. Invitrogen Cal-Lyse™ lysing solution is intended to be used as an aid in the enumeration of leukocytes that have been stained with monoclonal antibodies for flow cytometric analysis
Size: 100 ml
High-Yield Lyse is a premixed, fixative-free erythrocyte lysing solution that can be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. Using this reagent, lysis of erythrocytes is performed immediately following staining of the blood samples with Invitrogen's fluorochrome-conjugated monoclonal antibodies.
Size: 500 ml
Total Exosome Isolation (from serum) enables fast and efficient enrichment of intact exosomes from blood serum samples. • Maximise recovery of intact exosomes for any type of downstream applications • Easily isolate exosomes with our simple and reliable protocol • Avoid time-consuming ultra-centrifugation • Maximize flexibility—can be scaled up or down depending on the sample size Isolation of exosomes can be a tedious, non-specific, and difficult process. Total Exosome Isolation (from serum) enables simple and reliable concentration of intact exosomes from blood serum samples with a protocol ...
Size: 6 ml
This is the binding buffer used with MagVet™ Mycobacterium paratuberculosis Isolation Kit, validated for DNA extraction.
Size: 125 mL
This is a purified rabbit anti-human p50 oligoclonal antibody that can be use in the validated applications of indirect ELISA, Western blot, and immunocytochemistry. Antibody characteristics include: Applications: This p50 Oligoclonal Antibody, ABfinity™ Recombinant has been validated for use in indirect ELISA, Western blot and immunocytochemistry. Host species and isotype: This is a recombinant rabbit oligoclonal IgG against human p50. Clone ID of monoclonal antibody (mAb): The p50 oligoclonal antibody clone is 24HCLC. Reactivity: Along with human p50, this oligoclonal antibody is predicted t...
Size: 100 µg
This is a purified rabbit anti-human smooth muscle actin oligoclonal antibody that can be use in the validated applications of indirect ELISA and Western blot. Antibody characteristics include: Applications: This Smooth Muscle Actin Oligoclonal Antibody, ABfinity™ Recombinant has been validated for use in indirect ELISA and Western blot Host species and isotype: This is a recombinant rabbit oligoclonal IgG against human smooth muscle actin. Clone ID of oligoclonal antibody (mAb): The smooth muscle actin oligoclonal antibody clone is 17HCLC. Reactivity: Along with human smooth muscle actin , th...
Size: 100 µg
This purified Mouse Histidine (C-term) Tag Monoclonal Antibody recognizes a polyhistidine amino acid sequence at the carboxy-terminus of proteins. The free carboxy-terminus of the terminal histidine residue is a key element of the epitope recognition site of the anti-His (C-term) Antibody. The Anti-His(C-term) Monoclonal Antibody recognizes the sequence: -His-His-His-His-His-His-COOH . Validated applications for Histidine (C-term) Tag monoclonal antibody are western blotting and ELISA. Antibody Specifications: • Applications: Validated applications for Histidine (C-term) Tag Monoclonal Antibod...
Size: 50 µl
Goat whole molecule anti-human immunoglobulin Format/Formulation: HRP Conjugate
Size: 0.7 mg⁄ml
This purified Mouse anti-ErbB-2 Monoclonal Antibody is specific to human, rat, and mouse ErbB2 (HER2). Over-expression of c-erbB-2 is observed in 15–40% of primary human breast tumors and correlates with poor prognosis in node positive breast cancer. Over-expression of c-erbB-2 is also observed in ovarian, gastric, salivary, and non-small cell lung carcinomas. Validated applications for mouse anti-ErbB-2 monoclonal antibody are immunoprecipitation, immunohistochemistry, flow cytometry, and Western blotting. Antibody Attributes: • Applications: Validated applications for Mouse anti-ErbB-2 monoc...
Size: 100 µg