Cell Culture & Transfection Reagents Glutamine
Lipofectamine® RNAiMAX Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments. Lipofectamine® RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically for the delivery of siRNA and miRNA into all cell types. With Lipofectamine® RNAiMAX Transfection Reagent you will get: Superior transfection efficiency requiring lower RNAi concentrations leading to more effective gene knockdown with minimal non-specific effects Easy optimization due to minimal cytotoxicity across a 10-f...
Catalog: 13778-100
Size: 0.1 ml
Size: 0.1 ml
Lipofectin® Transfection Reagent is the reagent of choice for transfectin of endothelial cells; some of our customer also use it for transfection of DNA, RNA or oligonucleotides into their cell line of choice. Lipofectin® has also been shown to work well in combination with PLUS® reagent for the transfection of HeLa cells (1).
Catalog: 18292-011
Size: 1 ml
Size: 1 ml
PLUS™ Reagent is used in conjunction with transfection reagents, such as Lipofectamine™, to enhance transfection in adherent cell lines. Some customers find when using Plus™ Reagent that less optimization of DNA concentration is needed to achieve high transfection performance. The addition of Plus™ Reagent may also reduce the quantities of DNA and lipid required by approximately one-half compared to lipid alone. PLUS™ Reagent was specifically designed to expand the array of transfectable cell types (1). PLUS™ Reagent used with Lipofectamine™ Reagent has been demonstrated to improve the transfe...
Catalog: 11514-015
Size: 0.85 ml
Size: 0.85 ml
Lipofectamine® LTX Reagent offers a new, advanced solution for gene expression studies in hard-to-transfect cells. No other plasmid DNA-specific transfection reagent can match the efficiency, convenience, and gentleness of Lipofectamine® LTX Reagent for transfection of primary, difficult-to-transfect, and sensitive cell lines. Lipofectamine® LTX Reagent is synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in experiments or cell lines. A certificate of origin may be obtained by contacting Technical Support. ...
Catalog: A12621
Size: 0.1 ml
Size: 0.1 ml
A polyamine-based Ambion® siRNA transfection agent that can be used to transfect cells during subculturing-—saving a full day of valuable time—without increasing toxicity. It is supplied in one tube containing 0.4 mL. A successful siRNA experiment requires the appropriate transfection agent for your cells, and one that is optimized for siRNA delivery. The efficiency with which different transfection reagents deliver nucleic acids can vary dramatically with the type of nucleic acid being transfected (RNA vs. DNA, single-stranded vs. double-stranded) and the cells being used. Accessory Products:...
Catalog: AM4502
Size: 400 µl
Size: 400 µl
The FreeStyle™ MAX System is a breakthrough technology for rapid and high-yield mammalian protein production. This new mammalian expression technology is available in two convenient formats. FreeStyle™ MAX Reagent is a novel cationic lipid-based reagent designed to transfect FreeStyle™ cells with high efficiency. The FreeStyle™ MAX Expression System combines GIBCO® FreeStyle™ Media, FreeStyle™ MAX Reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293 cells. The only equipment required to use the FreeStyle™ MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinne...
Catalog: 16447-500
Size: 15 ml
Size: 15 ml
Invitrogen's Calcium Phosphate Transfection Kit provides high-quality reagents to enable the introduction of DNA into eukaryotic cells via calcium phosphate co-precipitation.
Catalog: K2780-01
Size: 75 reactions
Size: 75 reactions
The combination of the antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective action against gram-positive and gram-negative bacteria, respectively. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus . Streptomycin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibit...
Catalog: 15070-063
Size: 100 ml
Size: 100 ml
Fungizone® antimycotic is a formulation of Amphotericin B with sodium deoxycholate as an emulsifying agent to maintain the suspension. Amphotericin B is used to prevent the growth of fungi by causing an increase in fungal plasma membrane permeability. Gibco® Fungizone® antimycotic is used for the prevention of yeast and multicellular fungi contamination of cell cultures. Gibco® Fungizone contains 250 µg of amphotericin B and 205 µg of sodium deoxycholate per ml in distilled water. The recommended working concentration ranges from 0.25 to 2.50 µg/ml. Life Technologies offers a variety of antibi...
Catalog: 15290-018
Size: 20 ml
Size: 20 ml
Gibco® Zeocin™ selective reagent is a formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus . Gibco® Zeocin™ selective reagent acts by binding to and cleaving DNA, leading to cell death. Gibco® Zeocin™ selective reagent is used for selection of yeast, bacterial and mammalian cell cultures. This product is supplied at 100 mg/ml in sterile water and is used at a working concentration ranging between 50 - 400 µg/ml depending on the cell type. Life Technologies offers a variety of antibiotics and antimycotics for cell culture applications. Product Use For Res...
Catalog: R250-05
Size: 50 ml
Size: 50 ml
with L-Glutamine, EGF and BPE. Shelf life after supplementation: 2 weeks
Catalog: 17005-075
Size: 1 kit
Size: 1 kit
StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. Accutase® does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex™, CELLStart™ and polymers. Accutase® performs exceptionally well in detaching primary and stem cells and maintaining high cell viabilty compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase® treatment include analysis of cell su...
Catalog: A11105-01
Size: 100 ml
Size: 100 ml
Hygromycin B is a water-soluble antibiotic purified from the bacterium Streptomyces hydroscopicus . Hygromycin B acts by binding to the 80S subunit of the bacterial ribosome and inhibiting protein synthesis. Gibco® Hygromycin B is used as a bacterial selective antibiotic in the concentration range of 100 - 1000 µg / ml. This product is supplied as a 50 mg / ml solution. Resistance to Hygromycin B is conferred by the E. coli hygromycin resistance gene ( hyg or hph ). Hygromycin B uses a different mode of action than Geneticin®, Blasticidin S, or Zeocin™, so it is excellent for use in dual-selec...
Catalog: 10687-010
Size: 20 ml
Size: 20 ml
Geneticin® Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin® Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 - 200 µg / ml for bacteria, or 200 – 500 µg / ml for most mammalian cells. This product is supplied as a 50 mg / ml solution in...
Catalog: 10131-019
Size: 20 ml
Size: 20 ml
Geneticin® Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin® Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 - 200 µg / ml for bacteria, or 200 – 500 µg / ml for most mammalian cells. This product is supplied as a 50 mg / ml solution in...
Catalog: 10131-027
Size: 100 ml
Size: 100 ml
Geneticin® Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin® Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 - 200 µg / ml for bacteria, or 200 – 500 µg / ml for most mammalian cells. This product is supplied as a powder and should be m...
Catalog: 11811-031
Size: 5 g
Size: 5 g
Puromycin Dihydrochloride is an aminonucleoside antibiotic produced by Streptomyces alboniger . Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the "pac" gene. Puromycin is widely used in cell biology as a selective agent in mammalian cell culture systems. The recommended working concentration ranges from 0.2 – 5.0 µg/ml, although it can be toxic to eukaryotic cells at concentrations as low as 1 µg/ml. Gibco® Puromycin Dihydrochloride is supplied at 10 mg/mL in 20 mM HEPES buffer. Life Technologies offer...
Catalog: A11138-02
Size: 20 ml
Size: 20 ml
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • Low Glucose • L-glutamine • Sodium Pyruvate • Phenol Red • HEPES
Catalog: 11054-001
Size: 1 L
Size: 1 L
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • HEPES • L-glutamine • Phenol Red
Catalog: 12360-038
Size: 500 ml
Size: 500 ml
MCDB 131 Medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular endothelial cells (HUVEC). MCDB 131 Medium is also used with other cell types, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells. This MCDB 131 Medium is manufactured as follows: With Without • Phenol Red • L-glutamine • Sodium pyruvate • HEPES
Catalog: 10372-019
Size: 500 ml
Size: 500 ml
Cell Culture & Transfection Reagents Glutamine
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