Cell Counting Kits
The Violet Ratiometric Membrane Asymmetry Probe⁄Dead Cell Apoptosis Kit provides a simple and fast method for the detection of apoptosis with dead cell discrimination by flow cytometry. The Violet Ratiometric Membrane Asymmetry Probe, 4'-N,N-diethylamino-6-(N,N,N-dodecyl-methylamino-sulfopropyl)-methyl-3-hydroxyflavone (F2N12S), is a novel violet excitable dye for the detection of membrane asymmetry changes during apoptosis. The dye exhibits an excited-state intramolecular proton transfer (ESIPT) reaction resulting in a dual fluorescence with two emission bands corresponding to 530 nm and 585 ...
Catalog: A-35137
Size: 1 kit
Size: 1 kit
The LIVE/DEAD® Fixable Dead Cell Stain Kit employs an amine-reactive fluorescent dye to evaluate mammalian cell viability by flow cytometry. In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity between the live and dead cell populations is typically greater than 50 fold, and this fluorescence intensity discrimination is completely preserved fol...
Catalog: L-23105
Size: 1 kit
Size: 1 kit
The Tali® Cell Cycle Kit includes a ready-to-use reagent that, when used with the Tali® Image-Based Cytometer, provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle. This single-addition reagent is composed of propidium iodide (PI), RNase A, and Triton X-100. The Tali® Cell Cycle Kit offers a simple workflow: • The pre-mixed reagent can be stored at room temperature and used when your cells are ready • Simply fix your cells, add the reagent, incubate, and visualize • Cell cycle data can be analyzed on the Tali® instrument or exported as a ....
Catalog: A10798
Size: 50 assays
Size: 50 assays
Countess® Cell Counting Chamber Slides are designed for use with the Countess® Automated Cell Counter. Countess® slides are disposable. Each slide contains two cell counting chambers. This package comes with 1 box containing 50 slides and 2 x 1 mL vials of trypan blue (0.4%). Ready to Use For Your Convenience Each Countess® Cell Counting Chamber Slide comes presterilized and ready to use in an individual package. The Countess® slides are plastic and disposable. Cell counting occurs in a central location of the counting chamber. The volume counted is 0.4 µL, the same as counting four 1-mm 2 squ...
Catalog: C10228
Size: 1 box
Size: 1 box
The Vybrant® Cell Adhesion Assay Kit is a fast and sensitive assay for measuring cell-cell or cell-surface adhesion for a variety of cell types. In this assay, cells are labeled with calcein AM and allowed to adhere. After removal of nonadherent cells, calcein fluorescence is used to calculate the number of adherent cells.
Catalog: V-13181
Size: 1 kit
Size: 1 kit
The LIVE/DEAD® Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. Calcein violet AM and aqua-fluorescent reactive dye are optimal dyes for this application; both dyes utilize the violet laser allowing other laser lines to be used for conventional fluorochromes.
Catalog: L34958
Size: 1 kit
Size: 1 kit
The Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit contains a sensitive, one-step assay that uses the Amplex® Red reagent (10-acetyl-3,7-dihydroxyphenoxazine) to detect hydrogen peroxide (H 2 O 2 ) or peroxidase activity. The Amplex® Red reagent, in combination with horseradish peroxidase (HRP), has been used to detect H 2 O 2 released from biological samples, including cells, or generated in enzyme-coupled reactions. Furthermore, Amplex® Red reagent can be used as an ultrasensitive assay for peroxidase activity when H 2 O 2 is in excess. In the presence of peroxidase, the Amplex® Red reag...
Catalog: A22188
Size: 1 kit
Size: 1 kit
The Vybrant Apoptosis Assay Kit #4 detects apoptosis on the basis of changes that occur in the permeability of cell membranes. This kit contains ready-to-use solutions of both YO-PRO-1 and propidium iodide nucleic acid stains. YO-PRO-1 stain selectively passes through the plasma membranes of apoptotic cells and labels them with moderate green fluorescence. Necrotic cells are stained red-fluorescent with propidium iodide.
Catalog: V13243
Size: 1 kit
Size: 1 kit
The ApoTarget™ APO-BRDU Kit is a two-color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry.
Catalog: KHO1001
Size: 60 tests
Size: 60 tests
The ARCTURUS® HistoGene® LCM Immunofluorescence Staining Kit is designed to retrieve high-quality nucleic acids from immunofluorescently-stained, frozen tissue using a quick 15-minute process. The kit provides reagents and slides for convenient and reliable immunoflourescent staining as well as protocols that are streamlined and optimized to maintain nucleic acid quality. Key product features are: • Simplified staining—retrieve high-quality RNA from simplified fluorescent target cell labeling • Efficient preservation—quick 15-minute process preserves mRNA • Cold sample protection—chill samples...
Catalog: KIT0420
Size: 32 applications
Size: 32 applications
The SuperPicture™ Polymer Detection Kit features our proprietary and enhanced HRP polymer technology to provide excellent sensitivity, high specificity, and a shorter protocol than conventional immunohistochemical staining methods. Features of the SuperPicture™ Polymer Detection Kit: • Reactivity: Mouse, rabbit, guinea pig, and rat • Applications: Immunohistochemistry (FFPE) Our new polymer conjugation methods have resulted in a versatile polymer capable of intense nuclear, cytoplasmic, and membrane antigen staining. The polymer conjugate contains multiple HRP molecules to provide excellent se...
Catalog: 87-9663
Size: 1 kit
Size: 1 kit
The Premo™ Autophagy Sensor RFP-p62 Kit combines the ability to monitor the induction or inhibition of autophagy, through the localization of the autophagy receptor p62 (also known as SQSTM1), with the high transduction efficiency and minimal toxicity of BacMam 2.0 technology. To perform an image-based analysis for autophagy, simply add the BacMam 2.0 RFP-p62 reagent to your mammalian cells, incubate overnight to ensure adequate protein expression, and then visualize using standard RFP (red fluorescent protein) settings. The RFP gene included in this chimera is mKate2. This monomeric fluoresce...
Catalog: P36241
Size: 1 kit
Size: 1 kit
The ApoTarget™ Quick Apoptotic DNA Ladder Detection Kit provides a simple and rapid procedure for extraction of chromosomal DNA. The procedure prepares DNA for use in the methods that detect DNA fragmentation in apoptotic cells. Unlike other kits requiring 1 to 2 days to finish, this detection method requires only less than 90 minutes to prepare DNA in a single tube without the need for extraction or column steps. DNA fragmentation can be easily visualized by agarose gel electrophoresis. This procedure increases recovery of small fragmented DNA, thereby improving the sensitivity of the assay.
Catalog: KHO1021
Size: 50 tests
Size: 50 tests
The HistoMouse-Plus kit is the 2nd generation HistoMouse kit for the detection of mouse primary antibodies on mouse tissue samples without causing background due to endogenous mouse Ig. Our patented BEAT™ technology (Blocking Endogenous Antibody Technology) is used to prevent the secondary antibody conjugate from reacting with endogenous mouse Ig. The LAB-SA detection method is used to amplify the signal. In addition, our HistoMouse-Plus kit includes the AEC Single Solution chromogen⁄ substrate system to create an intense red deposit around the antigen⁄antibody⁄enzyme complex in the sample. Re...
Catalog: 85-9541
Size: For 50 Slides
Size: For 50 Slides
The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The kit includes the novel fluorogenic substrate CellEvent® Caspase-3/7 Green Detection Reagent, as well as SYTOX® AADvanced™ Dead Cell Stain. • Caspase-3/7 specific—includes the recognition sequence for activated caspase-3 and caspase-7 • Easy identification—clearly identify live, dead, and apoptotic cell populations • Quick analysis—washing and fixation is not required • Multicolor compatibility—combine with other dyes excitable by the 488 n...
Catalog: C10427
Size: 100 assays
Size: 100 assays
The Premo™ Autophagy Sensor GFP-p62 Kit combines the ability to monitor the induction or inhibition of autophagy, through the localization of the autophagy receptor p62 (also known as SQSTM1), with the high transduction efficiency and minimal toxicity of BacMam 2.0 technology. To perform an image-based analysis for autophagy, simply add the BacMam 2.0 GFP-p62 reagent to your mammalian cells, incubate overnight to ensure adequate protein expression, and then visualize using standard GFP (green fluorescent protein) settings. The GFP gene included in this chimera is TagGFP2, which has been demons...
Catalog: P36240
Size: 1 kit
Size: 1 kit
The Premo™ Autophagy Tandem Sensor RFP-GFP-LC3B Kit combines the ability to monitor the various stages of autophagy (through LC3B protein localization) with the high transduction efficiency and minimal toxicity of BacMam 2.0 expression technology. To perform image-based analysis for autophagy, simply add the BacMam 2.0 RFP-GFP-LC3B reagent to your mammalian cells, incubate overnight to ensure maximum protein expression, and then visualize using standard GFP (green fluorescent protein) and RFP (red fluorescent protein) settings. This tandem RFP-GFP sensor capitalizes on the pH difference betwee...
Catalog: P36239
Size: 1 kit
Size: 1 kit
StemPro® CD34+ cells are pooled Human Hematopoietic Progenitor cells (HPCs) derived from the umbilical cord blood of mixed donors. Along with 0.5×10 6 pooled donor cord blood-derived CD34+ cells, this kit includes StemPro®-34 SFM basal liquid medium and frozen StemPro®-34 Nutrient Supplement to facilitate the immediate culturing of the StemPro® CD34+ cells upon arrival or when recovering from cryopreservation storage. With the StemPro® CD34+ Cell Kit You Get: • ≥90% purity of the desired CD34+ cell population • Each vial of cells contains 0.5×10 6 CD34+ cells with full traceability • StemPro®-...
Catalog: A14059
Size: 0.5 x 10
Size: 0.5 x 10
Use this kit to isolate a high purity and yield of human CD34+ progenitor stem cells. Positively isolated cells are bead and antibody-free, phenotypically unaltered and suitable for any downstream applications including flow cytometry, functional studies, and cell culture to produce dendritic cells (DC's). The Dynal® CD34 Progenitor Cell Isolation System can also be used to isolate CD34+ cells from rhesus monkey (Macaca mulatta). Positive isolation of human CD34+ progenitor stem cells with bead release Stem cells can be isolated directly from whole blood, cord blood or bone marrow Isolated CD3...
Catalog: 11301D
Size: 5 ml
Size: 5 ml
Invitrogen's Calcium Phosphate Transfection Kit provides high-quality reagents to enable the introduction of DNA into eukaryotic cells via calcium phosphate co-precipitation.
Catalog: K2780-01
Size: 75 reactions
Size: 75 reactions
Cell Counting Kits
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