cdna synthesis kits & assays
The SuperScript® Double-Stranded cDNA Synthesis Kit contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality double-stranded cDNA from total RNA or poly A + selected RNA (mRNA). The advanced capabilities of SuperScript® II Reverse Transcriptase, with its reduced RNase H activity, maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis. Features of the SuperScript® Double-Stranded cDNA Synthesis Kit include: • Reliability —Each buffer, reagent, and enzyme in the kit is of the same high quality you expect from Life Technologie...
Catalog: 11917-010
Size: 10 reactions
Size: 10 reactions
The NCode™ VILO™ miRNA cDNA Synthesis Kit is part of a novel quantitative assay that provides flexibility, sensitivity, and specificity when quantitating miRNA expression. The system combines a carefully optimized polyadenylation reaction with the market-leading reverse transcriptase, SuperScript® III RT, in a universal first-strand cDNA synthesis reaction. Tried and tested SuperScript® III Reverse Transcriptase is formulated in an enhanced buffer system to provide you with the most reliable firststrand synthesis and higher cDNA yields, with sensitivity and accurate quantitation from 10pg to 1...
Catalog: A11193-050
Size: 50 reactions
Size: 50 reactions
The High Capacity RNA-to-cDNA™ Kit is a streamlined reverse transcription kit designed for optimum performance with the TaqMan® Gene Expression Master Mix, Power SYBR® Green PCR Master Mix, and other PCR enzymes. Features of the High Capacity RNA-to-cDNA™ Kit: • Fast —Short reaction time (typically 30–60 min) • Convenient —Easy workflow with few pipetting steps (2 tubes) • Reliable —Reliable reverse transcription of both abundant and limited targets • Flexible —Optimized 2-step protocol enabling multiple PCR reactions from a single reverse transcription reaction Streamline Your cDNA Synthesis ...
Catalog: 4387406
Size: 50 reactions
Size: 50 reactions
The SuperScript® III First-Strand Synthesis System for RT-PCR provides all the necessary components for synthesis of high-quality first-strand cDNA from total or poly(A)+ RNA. Used with PCR, this functionally tested system enables you to detect rare messages and generate enough material for cloning. The SuperScript® III First-Strand Synthesis System: Uses SuperScript® III RT for higher cDNA yields (Figure 1) Includes all components needed for first-strand cDNA synthesis Consists of optimized reagents for more robust performance Contains a streamlined protocol Detects template molecules from as...
Catalog: 18080-051
Size: 50 reactions
Size: 50 reactions
The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of input total RNA in a single 20 µL reaction to single stranded cDNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Includes sufficient reagents for 1000 x 20 µL reactions. • Linear target amplification for real-time PCR • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost • 10-fold greater dynamic range than other kits cDNA from Total RNA The High Capacity cDNA Reverse Transcription Kit (for...
Catalog: 4374967
Size: 1000 reactions
Size: 1000 reactions
SuperScript® First-Strand Synthesis System for RT-PCR is optimized for sensitive RT-PCR from low-input RNA, Taq or Platinum® Taq DNA Polymerase for higher specificity PCR or with AccuPrime™ Pfx DNA Polymerase for high-fidelity cloning applications. The SuperScript® First-Strand Synthesis System for RT-PCR: Is optimized for RT-PCR Uses SuperScript® II RT for greater first-strand cDNA yields Detects as little as 50 to 100 molecules of RNA template from only 1 pg of total RNA (Figure 1) Includes oligo(dT) and random hexamers to prime cDNA synthesis for flexible use Amplifies first-strand cDNA pro...
Catalog: 11904-018
Size: 50 reactions
Size: 50 reactions
The SuperScript® III First-Strand Synthesis SuperMix provides high yields of first-strand cDNA in a convenient high-throughput supermix format. The simple, time-saving reaction set-up uses just two tubes-a 2X Reaction Mix and a SuperScript® III Enzyme Mix (Figure 1). An optional annealing buffer is provided for improved yields and sensitivity. The Enzyme Mix includes SuperScript® III Reverse Transcriptase to ensure higher cDNA yields, superior sensitivity-down to 0.1 pg (Figure 2)-and specificity, and compatibility with a wide variety of RT-PCR applications.
Catalog: 18080-400
Size: 50 reactions
Size: 50 reactions
The Applied Biosystems® TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for 200 reactions of optimal performance in TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA. A highly specific kit that quantitates only mature miRNAs, not precursors. A sensitive kit that conserves limited samples and requires only 1 to 10 nanograms of total RNA. It is fast, simple and scalable 2-step quantitative RT-PCR assay that provides high-quality results in less than three hours.
Catalog: 4366596
Size: 200 reactions
Size: 200 reactions
This Applied Biosystems® kit provides the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, and oligo (dT) 16 , reverse primers are included for cDNA synthesis flexibility. Supplied as 2000 reactions in 10 tubes (200 reactions/tube). • Contains MultiScribe® Reverse Transcriptase, a recombinant Moloney Murine Leukemia Virus Reverse Transcriptase • Random hexamers and oligo (dT) 16 included for flexibility • Individual components provide versatility in assay set-up • Compatible with one-step or two-step RT-PCR reactions TaqMan® Reverse Transcription Reagents provide all...
Catalog: 4304134
Size: 2000 reactions
Size: 2000 reactions
This kit provides the indivdual components to perform reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and sequence-specific reverse primers provide flexibility for cDNA synthesis. • Contains MultiScribe® Reverse Transcriptase, a recombinant Moloney Murine Leukemia Virus Reverse Transcriptase • Random hexamers and oligo (dT)16 provide flexibility for cDNA synthesis • Individual components provide versatility in assay set-up • Compatible with one-step or two-step RT-PCR reactions Life Technologies has developed a comprehensive set of guidelines to ensure success when using Rea...
Catalog: N8080234
Size: 200 reactions
Size: 200 reactions
The Gateway® Vector Conversion System is designed to convert any vector into a Gateway® destination vector using restriction endonucleases and ligase. A Gateway® cassette containing att R recombination sites flanking a ccd B gene (1) and a chloramphenicol-resistance gene are blunt-end cloned into the multiple cloning site of any vector. ccdB Survival 2 competent cells allow propagation of the Gateway® vectors containing the ccd B gene. The Gateway® Vector Conversion System offers: • Conversion of any expression vector into a Gateway® destination vector • Three cassettes to construct a destinat...
Catalog: 11828-029
Size: 1 kit
Size: 1 kit
cdna synthesis kits & assays
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