Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > DNA Ladders
The TrackIt™ format combines the most popular DNA Ladders with loading buffer in a single tube. This format minimizes time spent thawing, diluting, and adding sample buffer to your DNA ladders. Simply transfer your TrackIt™ DNA Ladder from the vial to the gel. These ladders are stable at room temperature and can be stored on your bench top. TrackIt™ DNA Ladders offer two tracking dyes, one that runs behind the sample and one that runs ahead of the sample. The tracking dyes serve as visual markers for following migration during electrophoresis and indicate when maximum resolution of the DNA fra...
Catalog: 10488-022
Size: 20 applications
Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > DNA Ladders
1 Kb Plus DNA ladder is a powerful tool for estimating the molecular weight of linear, double stranded DNA fragments. 1 Kb Plus DNA ladder is composed of 20 highly purified, double stranded DNA bands spanning 100 base pairs to 12,000 base pairs. 1 Kb Plus DNA ladder has 12 evenly spaced bands ranging from 1 Kb to 12 Kb, a quick orientation band at 1650 base pairs that forms a distinct doublet with the 2 Kb band, and seven bands of round sizes below 1 Kb. 1 Kb Plus DNA ladder is compatible with agarose gels you cast in your lab as well as E-Gel® pre-cast agarose gels. Round, easy to remember ba...
Catalog: 10787-026
Size: 1000 µg
Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > RNA Ladders
0.5-10 Kb RNA Ladder is suitable for sizing single-stranded RNA ranging from 0.5 kb to 10 kb in glyoxal or formaldehyde agarose gels. The ladder consists of nine bands in even increments (0.5, 1, 1.5, 2, 3, 4, 6, 8, and 10 kb) to simplify size estimation. The 1.5 kb band is more intense than the other bands, allowing easy orientation and identification of RNA bands on the gel. All RNA molecules in the ladder are polyA-tailed (40 nt) for convenient use as control templates for cDNA synthesis.
Catalog: 15623-200
Size: 75 µg
Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > DNA Ladders
1 Kb Plus DNA ladder is a powerful tool for estimating the molecular weight of linear, double stranded DNA fragments. 1 Kb Plus DNA ladder is composed of 20 highly purified, double stranded DNA bands spanning 100 base pairs to 12,000 base pairs. 1 Kb Plus DNA ladder has 12 evenly spaced bands ranging from 1 Kb to 12 Kb, a quick orientation band at 1650 base pairs that forms a distinct doublet with the 2 Kb band, and seven bands of round sizes below 1 Kb. 1 Kb Plus DNA ladder is compatible with agarose gels you cast in your lab as well as E-Gel® pre-cast agarose gels. Round, easy to remember ba...
Catalog: 10787-018
Size: 250 µg
Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > DNA Ladders
The 1 Kb DNA Extension Ladder is suitable for sizing linear double-stranded DNA fragments from 500 bp to 40 kb. The ladder consists of 8 fragments ranging in length from 1,018 to 8,144 bp in 1,018-bp increments, as well as bands of 506 bp, 517 bp, 1,636 bp, 10 kb, 15 kb, 20 kb, and 40 kb. For easy reference on agarose gels, the 5 kb band is two to three-times brighter than the other bands in the ladder. This ladder can be stained with ethidium bromide or radiolabeled using T4 polynucleotide kinase, T4 DNA polymerase, DNA polymerase I, or the large fragment DNA polymerase I (Klenow fragment).
Catalog: 10511-012
Size: 100 µg
Category: Products & Services > Controls, Standards & Ladders > Nucleic Acid Ladders > RNA Ladders
0.1-2 Kb RNA Ladder is suitable for sizing single-stranded RNA from 0.1 to 2 kb in glyoxal or formaldehyde agarose gels. The ladder consists of ten bands in even increments (0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.8, 1.0, 1.5, and 2.0 kb) to simplify size estimation. The 0.3 kb band is more intense than the other bands, allowing easy orientation and identification of RNA bands on the gel. All RNA molecules in the ladder are polyA-tailed (40 nt) for convenient use as control templates for cDNA synthesis.
Catalog: 15623-100
Size: 75 µg
Category: Products & Services > Controls, Standards & Ladders > Protein Standards & Ladders > Prestained Protein Standards
The BenchMark™ Pre-Stained Protein Ladder is suitable as a standard to monitor electrophoresis or electrophoretic transfer from the gel to a membrane in western blots (1). It consists of 10 proteins ranging in apparent molecular weight from ~10 to 190 kDa. It is designed for use with Tris-Glycine Gels. The BenchMark™ Pre-Stained Protein Ladder offers: Proteins covalently bound to a blue dye for easy visualization The fourth protein band from the top coupled with a pink dye for easy orientation and to ensure proper identification of each protein Affinity-purified proteins to provide sharp bands...
Catalog: 10748-010
Size: 2 × 250 µl
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Apoptosis Kits
The ApoTarget™ Quick Apoptotic DNA Ladder Detection Kit provides a simple and rapid procedure for extraction of chromosomal DNA. The procedure prepares DNA for use in the methods that detect DNA fragmentation in apoptotic cells. Unlike other kits requiring 1 to 2 days to finish, this detection method requires only less than 90 minutes to prepare DNA in a single tube without the need for extraction or column steps. DNA fragmentation can be easily visualized by agarose gel electrophoresis. This procedure increases recovery of small fragmented DNA, thereby improving the sensitivity of the assay.
Catalog: KHO1021
Size: 50 tests
Category: Products & Services > Controls, Standards & Ladders > Control Serum, Cell Extracts, & Related Standards
Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Buffer: PBS Preservative: 0.1% Sodium Azide
Catalog: 10000C
Size: 25 ml
Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > DNA Sequencing & Matrix Standards
The BigDye® Terminator v3.1 sequencing standard kit contains four tubes, each of which contains enough DNA of a known 1200 base pair sequence prepared with BigDye® Terminator v3.1 to perform one spectral calibration⁄performance check and⁄or one control sequencing run on the Applied Biosystems® 3500 Series Genetic Analyzer. The DNA contained in the preparation has been lyophilized to maximize stability. For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Catalog: 4404312
Size: 4 tubes
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® TaqMan® Rodent GAPDH Control Reagents provide the components for using rodent GAPDH as a normalization control in applications with rat, mouse, and Chinese hamster cells. • Pre-designed primers and TaqMan® probe eliminate assay design • Rapid assay development guidelines can minimize optimization time • Separate components provide flexibility in assay set-up Comprehensive Guidelines Included Applied Biosystems® has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems® Real-Time PCR reagents and instrumentation. These guidelines are...
Catalog: 4308313
Size: 1000 reactions
Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Positive Controls
Ambion® Silencer ® Firefly Luciferase (GL2 + GL3) Positive Control siRNA is ideal for developing and optimizing siRNA experiments. The control is validated for use in human cell lines and optimized for mouse and rat. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to...
Catalog: AM4629
Size: 5 nmol
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
Human TaqMan® Copy Number Reference Assays are run with human TaqMan® Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome. Two options for genes that can be used as endogenous reference genes in humans are offered: RNase P and TERT. TaqMan® Copy Number Reference Assays are designed to unique genomic sequences in the reference genome assembly (NCBI Build 37/hg19) and are required for relative quantitation of copy number targets. TaqMan® Copy Number Assays quantitate the gene of interest and normalize t...
Catalog: 4403326
Size: 750 reactions
Category: Products & Services > Controls, Standards & Ladders > Control Serum, Cell Extracts, & Related Standards
Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Preservative: 0.1% Sodium Azide
Catalog: 10510
Size: 10 ml
Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Negative Controls
Ambion® In Vivo siRNA Controls • Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts • Positive Control siRNAs functionally tested in several common cell lines • Negative Controls functionally proven to have minimal effects on cell proliferation and viability • Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability • For use in human, mouse, and rat cells What are Ambion® In Vivo siRNAs? Ambion® In Vivo siRNAs are designed using the proven Silencer ® Select algorithm and incorpora...
Catalog: 4457287
Size: 5 nmol
Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > DNA Sequencing & Matrix Standards
The DS-30 Matrix Standard kit contains 4 oligonucleotides respectively labeled with 6-FAM™, HEX™, NED™, and ROX™, pre-pooled in a single tube. This Matrix Standard Set is used to generate the "multicomponent matrix" required when analyzing fluorescent dye-labeled DNA fragments on the Applied Biosystems® 3100, 3130, and 3730 Series Systems. The Data Collection Software for these instruments uses the multicomponent matrix to automatically analyze the four different colored fluorescent dye-labeled samples in a single capillary. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4345827
Size: 8 runs
Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates
The Applied Biosystems® Mouse ACTB (actin, beta) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when it is used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. It can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes. Assay Details: Gene Symbol: Actb Re...
Catalog: 4352341E
Size: 1000 reactions
Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Positive Controls
Ambion® In Vivo siRNA Controls • Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts • Positive Control siRNAs functionally tested in several common cell lines • Negative Controls functionally proven to have minimal effects on cell proliferation and viability • Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability • For use in human, mouse, and rat cells What are Ambion® In Vivo siRNAs? Ambion® In Vivo siRNAs are designed using the proven Silencer ® Select algorithm and incorpora...
Catalog: 4459407
Size: 50 nmol
Category: Products & Services > Controls, Standards & Ladders > Control Serum, Cell Extracts, & Related Standards
Class: RUO Statement: For research use only. Not for use in diagnostic procedures. Preservative: 0.1% Sodium Azide
Catalog: 10410
Size: 10 ml