Bigdye Manuals

6100 PrepStation Users Manual

Category: Products & Services > Literature > Manuals > User Guides, Manuals & Bulletins

This is a User Guide for the ABI PRISM™ 6100 Nucleic Acid PrepStation. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4326244
Size: 1 manual

Quantifiler® Duo DNA Quantification Kit User's manual

Category: Products & Services > Literature > Manuals > User Guides, Manuals & Bulletins

This is the user's manual for the Quantifiler® Duo DNA Quantification Kit. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4391294
Size: 1 manual

BigDye® Terminator v1.1 & v3.1 5X Sequencing Buffer

Category: Products & Services > Buffers & Chemicals > Sequencing Buffers & Reagents

This sequencing buffer is optimized for use with BigDye® Terminator v1.1 and v3.1 Cycle Sequencing Kits. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4336701
Size: 233 ml

3500/3500xL Sequencing Standards, BigDye® Terminator v3.1

Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > DNA Sequencing & Matrix Standards

The BigDye® Terminator v3.1 sequencing standard kit contains four tubes, each of which contains enough DNA of a known 1200 base pair sequence prepared with BigDye® Terminator v3.1 to perform one spectral calibration⁄performance check and⁄or one control sequencing run on the Applied Biosystems® 3500 Series Genetic Analyzer. The DNA contained in the preparation has been lyophilized to maximize stability. For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Catalog: 4404312
Size: 4 tubes

BigDye® Terminator v3.1 Cycle Sequencing Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337458
Size: 25000 reactions

BigDye® Terminator v3.1 Cycle Sequencing Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337457
Size: 5000 reactions

MEGAshortscript™ T7 Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of large mass amounts of RNA produced from small templates ( • Efficiently transcribes small templates ( • Designed for use with plasmid, synthetic oligonucleotide, or PCR product templates Short transcripts require many more transcription initiation events to synthesize a given mass of product than do longer transcripts. Since the initiation step is the rate-limiting step in transcription reactions, the yield of product from short templates is often low. The MEGAshortscript™ High Yield Tra...
Catalog: AM1354M
Size: 25 rxns w⁄ Manual

MEGAscript® T7 Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions. Each Ambion® kit includes sufficient reagents for 25 reactions. • Exclusive, ultrahigh yield technology is fast: 2 hour reaction time • Amplifies aRNA for gene array analysis and other applications • Efficiently incorporates many modified nucleotides A typical 20 µl MEGAscript® reaction with 1 µg of the pTRI-Xef-1alpha control template will yield over 100 µg of transcript. The versatility of the MEGAscript® k...
Catalog: AM1333M
Size: 25 rxns w⁄ Manual

mMESSAGE mMACHINE® T7 Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. • High yields of capped RNA; up to 39 µg of RNA per reaction • Extra convenience; cap analog-nucleotide mix provided • Simplified reaction format • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped...
Catalog: AM1344M
Size: 25 rxns w⁄ Manual

Ambion® WT Expression Kit, Manual Use

Category: Products & Services > Kits & Assays > RNA Amplification Kits

The WT Expression Kit is an RNA amplification innovation from Ambion® that is designed to generate amplified sense-strand cDNA ready for fragmentation and labeling using the Affymetrix® GeneChip® WT Terminal Labeling Kit. Features of the Ambion® WT Expression Kit: • Optimized for use with Affymetrix® GeneChip® Human, Mouse, and Rat 1.0 ST Arrays • Allows RNA samples as small as 50 ng to be analyzed on Affymetrix® GeneChip® Arrays • Supports a streamlined workflow that does not require a separate rRNA depletion step • Is ideal for high-sensitivity expression profiling Eliminates Separate rRNA D...
Catalog: 4411974
Size: 30 reactions

pMIR-REPORT™ miRNA Expression Reporter Vector System with Manual

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > RNAi Vectors

The Ambion® pMIR-REPORT™ miRNA Expression Reporter Vector System is for the measurement of miRNA expression in cells. It contains two mammalian expression vectors-—one for cloning miRNA targets and evaluating miRNA regulation, and the other for the normalization of transfection efficiency. Features of the pMIR-REPORT™ miRNA Expression Reporter Vector System: • Clone miRNA targets and evaluate miRNA regulation • Screen putative miRNA target sequences • Includes both reporter and control vectors The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell mea...
Catalog: AM5795M
Size: 1 kit w⁄ Manual

MEGAclear™ Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits

The Ambion® MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. MEGAclear™ efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Each kit includes sufficient reagents for 20 reactions. • Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components • High recovery from 1 ng to 500 µg of input • Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections • Quick protocol—purify RNA in le...
Catalog: AM1908M
Size: 20 rxns w⁄ Manual

mMESSAGE mMACHINE® T3 Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. • High yields of capped RNA—up to 39 µg of RNA per reaction • Extra convenience—cap analog-nucleotide mix provided • Simplified reaction format • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped R...
Catalog: AM1348M
Size: 25 rxns w⁄ Manual

DNA Zap ™ (250 ml each) with Manual

Category: Products & Services > Buffers & Chemicals > Nucleic Acid Storage & Nuclease Control Reagents > Nuclease Control Reagents

Ambion® DNA Zap ™ (patent pending) consists of two solutions that are innocuous when used alone, but become a potent nucleic acid degrading solution when mixed. This mixture is able to instantaneously degrade high levels of contaminating DNA and RNA from surfaces. 250 mL of each solution are provided. • Completely degrades contaminating DNA and RNA at the level of PCR sensitivity • Works on contact • Degrades nucleic acid, unlike other products on the market that only act as detergents • Ideal for cleaning PCR tubes, PCR machine surfaces, pipettors, lab benches, lab equipment, and microfuge tu...
Catalog: AM9890M
Size: 250 ml (Manual)

BigDye® Terminator v3.1 Cycle Sequencing Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337456
Size: 1000 reactions

mMESSAGE mMACHINE® SP6 Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. • High yields of capped RNA • Extra convenience; cap analog-nucleotide mix provided • Simplified reaction format • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped RNA Transcription will yield lar...
Catalog: AM1340M
Size: 25 rxns w⁄ Manual

TURBO DNA- free ™ Kit with Manual

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > DNA⁄RNA Modifying Enzymes

The TURBO DNA- free ™ Kit contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. Features of the Ambion® TURBO DNA- free ™ Kit include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can interfere with RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The best method for genomic DNA removal prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNase I that is much more efficient t...
Catalog: AM1907M
Size: 50 rxns w⁄ Manual

ABI PRISM 3100-Avant Genetic Analyzer User Guide

Category: Products & Services > Literature > Manuals > User Guides, Manuals & Bulletins

This is a user guide for the ABPI PRISM 3100- Avant Genetic Analyzer. Important Note: 3100 System Sequencers will be retired at the end of 2011. Take advantage of our limited time promotion to either TRADE-IN your 3100 for a brand new 3500 or UPGRADE to a 3130. Please contact your local Sales Representative to learn more.
Catalog: 4333549
Size: 1 each

AmpFLSTR® Identifiler® Plus PCR Amplification Kit User Guide

Category: Products & Services > Literature > Manuals > User Guides, Manuals & Bulletins

This is the User Guide Manual for the AmpFLSTR® Identifiler® Plus PCR Amplification Kit. Note: For Forensic or Paternity Use Only.
Catalog: 4440211
Size: 1 manual

Site Prep and Safety Guide, Sequence Detection System 7900HT

Category: Products & Services > Literature > Manuals > User Guides, Manuals & Bulletins

This is the Site Prep and Safety Guide for the 7900HT Sequence Detection System. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4317595
Size: 1 unit