Biotin-14-dATP is a patented dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker. The long, 14-atom spacer arm allows accessibility to streptavidin for sensitive detection of probe-target hybrids in in situ and filter-based hybridizations. Biotin-14-dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP, dGTP, and dTTP (1). Biotin-14-dATP can be added to the 3´ end of DNA using terminal deoxynucleotidyl transferase (2). The amount supplied is sufficient for labeling up to 50 µg of DNA by nick translation. Performance and ...
Size: 50 nmol
CaptAvidin™ biotin-binding protein contains a nitrated tyrosine in its biotin-binding site. This chemical modification permits dissociation of the avidin-biotin complex under milder conditions than the traditional avidin-biotin complex. At pH 4.0, CaptAvidin™ biotin-binding protein binds biotin tightly with a K a of ~10 9 M -1 . This association can then be reversed at pH 10, allowing complete dissociation of the avidin-biotin complex. This is in contrast to the harsh conditions (8 M guanidine hydrochloride, pH 1.5) needed to dissociate the avidin-biotin complex.
Size: 1 mg
Our NeutrAvidin® biotin-binding protein - a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point - can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding.
Size: 5 mg
The amine-reactive PyMPO succinimidyl ester can be used to create bioconjugates with this environment-sensitive fluorophore.
Size: 5 mg
The Vybrant Apoptosis Assay Kit #4 detects apoptosis on the basis of changes that occur in the permeability of cell membranes. This kit contains ready-to-use solutions of both YO-PRO-1 and propidium iodide nucleic acid stains. YO-PRO-1 stain selectively passes through the plasma membranes of apoptotic cells and labels them with moderate green fluorescence. Necrotic cells are stained red-fluorescent with propidium iodide.
Size: 1 kit
Goat whole molecule anti-human immunoglobulin Format/Formulation: Biotin Conjugate
Size: 0.7 mg⁄ml
TCR ALPHA BETA, Hamster Anti-Mouse, (Alexa Fluor 488); Alexa Fluor 488 conjugated Monoclonal antibody specific to Mouse TCR α/β. This antibody is validated for use in Flow Cytometry. Anti-TCR α/β recognizes the expressed product of the TCRA/B gene.
Size: 1 ml
Mouse anti-Galectin-1; Monoclonal antibody specific to Human, mouse, swine, rat, ovine, hamster, bovine Galectin-1.This antibody is validated for use in Western Blot, Immunocytochemistry/ Immunofluorescence.
Size: 100 µg
PI4KB and PI4KA are the two human type III PI4 kinases, which share sequence similarly with PI3 kinases and phosphorylate the 4 position of phosphotidyl inositol.
Size: 20 µg
This purified mouse anti-NY-ESO-1 monoclonal antibody recognizes human NY-ESO-1. NY-ESO-1 is a member of the cancer/testis (CT) family of antigens, which are expressed in a range of malignant neoplasmas such as melanoma and carcinomas of the breast, ovary and lung, but not in normal tissue except in testis. NY-ESO-1 is a classic CT antigen discovered during a SEREX analysis of esophageal cancer. It is a 22–23 kDa hydrophobic protein. Validated applications for anti-NY-ESO-1 are western blotting and immunohistochemistry (paraffin). Antibody Specifications: • Applications: Validated applications...
Size: 100 µg
Clone/PAD: Z021 Immunogen: Synthetic biotin Specificity: Binds strongly to biotinylated proteins. Reactivity: Biotinylated proteins Applications: ELISA, Cell and tissue staining
Size: 1 ml
F4/80, Rat Anti-Mouse, (Biotin); Biotinylated Monoclonal antibody specific to Mouse F4/80. This antibody is validated for use in Flow Cytometry. Anti-F4/80 recognizes the expressed product of the Emr1 gene.
Size: 1 ml
PKA REG 11BETA [pS114) PAb; Unconjugated Polyclonal antibody specific to Mouse, (Human, Rat) PKA IIβ [pS114] (regulatory domain). This antibody is validated for use in Western Blot. Anti-PKA IIβ [pS114] (regulatory domain) recognizes the expressed product of the PRKAR2B gene. Catalog number 44998G is same antibody as former catalog number 44998. 44998G contains 50% glycerol, which improves the stability and longevity of this antibody.
Size: 10 blots
Clone/PAD ZMD.667 Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phosphoserines 33 and 37 of human β-catenin. Specificity Specific for the ~83 kDa β-catenin phosphorylated at serines 33 and 37. Reactivity The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will react with mouse, rat and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used Applications Western blotting. Other applications have not been tested References 1. Ding Y, Dale, T, Trends B...
Size: 100 µl
The CD90/Thy-1 (clone G7) R-PE (phycoerythrin) conjugate is a monoclonal rat antibody that recognizes the Thy-1.1 and Thy-1.2 alloantigens (Thy-1 epitope region A). R-PE is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thereby affording exceptional quantum yields and molar extinction coefficients. The use of PE in antibody conjugates provides probes with greatly enhanced detectability. Antibody Specifications Description: CD90/Thy-1, Rat IgG 2c monoclonal Label: R-PE (phycoer...
Size: 25 µg
This is a purified rabbit anti-human Lipocalin-1 oligoclonal antibody (oAb) that can be used in validated applications such as western blotting (WB), immunocytochemistry (ICC), and indirect ELISA. ABfinity™ antibody characteristics include: • APPLICATIONS: This Lipocalin-1 Oligoclonal Antibody, ABfinity™ Recombinant has been validated for use in western blotting, immunocytochemistry, and indirect ELISA. • HOST SPECIES AND ISOTYPE: This is a recombinant rabbit oligoclonal IgG against human Lipocalin-1. • CLONE ID OF OLIGOCLONAL (oAb): The oligoclonal antibody clone is 14HCLC. • REACTIVITY: Alon...
Size: 100 µg
Ambion® Molecular biology grade, 1M Tris, pH 7.0 solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contamina...
Size: 500 ml
Gibco® Trypsin 1:250 powder is an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength it is widely used for cell dissociation, during routine cell culture passaging, and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing Quality Testing Gibco® Trypsin 1:250 is tested for endotoxin, mycoplasma, bacterial, fungal and viral contamination. Other quality tests include multiple activity assays, ash analysis and mois...
Size: 100 g
The Ion Xpress™ Barcode Adapters 1-16 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Ba...
Size: 1 kit
Applied Biosystems® TaqMan® Universal PCR Master Mix, No AmpErase® UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® re...
Size: 2000 reactions