®-based reagents

ActinRed™ 555 ReadyProbes™ Reagent

Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents

ActinRed™ 555 ReadyProbes™ Reagent is a selective, high-affinity F-actin probe conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). Like other ReadyProbes™ reagents, ActinRed™ 555 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells. ActinRed™ 555 ReadyProbes™ Reagent provides: • High-affinity staining of F-actin with superior specificity compared to antibody methods • Outstanding fluorescence performance (Ex/Em: 540/565 nm) • Ready-to-use liquid formulation in convenie...
Catalog: R37112
Size: 1 kit

ActinGreen™ 488 ReadyProbes™ Reagent

Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents

ActinGreen™ 488 ReadyProbes™ Reagent is a selective, high-affinity F-actin probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. Like other ReadyProbes™ reagents, the ActinGreen™ 488 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells. ActinGreen™ 488 ReadyProbes™ Reagent provides: • High-affinity staining of F-actin with superior specificity compared to antibody methods • Outstanding fluorescence performance (Ex/Em: 495/518 nm) • Ready-to-use liquid formulati...
Catalog: R37110
Size: 1 kit

PLUS™ Reagent

Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents

PLUS™ Reagent is used in conjunction with transfection reagents, such as Lipofectamine™, to enhance transfection in adherent cell lines. Some customers find when using Plus™ Reagent that less optimization of DNA concentration is needed to achieve high transfection performance. The addition of Plus™ Reagent may also reduce the quantities of DNA and lipid required by approximately one-half compared to lipid alone. PLUS™ Reagent was specifically designed to expand the array of transfectable cell types (1). PLUS™ Reagent used with Lipofectamine™ Reagent has been demonstrated to improve the transfe...
Catalog: 11514-015
Size: 0.85 ml

Lipofectamine® RNAiMAX Transfection Reagent

Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents

Lipofectamine® RNAiMAX Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments. Lipofectamine® RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically for the delivery of siRNA and miRNA into all cell types. With Lipofectamine® RNAiMAX Transfection Reagent you will get: Superior transfection efficiency requiring lower RNAi concentrations leading to more effective gene knockdown with minimal non-specific effects Easy optimization due to minimal cytotoxicity across a 10-f...
Catalog: 13778-100
Size: 0.1 ml

FreeStyle™ MAX Reagent

Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents

The FreeStyle™ MAX System is a breakthrough technology for rapid and high-yield mammalian protein production. This new mammalian expression technology is available in two convenient formats. FreeStyle™ MAX Reagent is a novel cationic lipid-based reagent designed to transfect FreeStyle™ cells with high efficiency. The FreeStyle™ MAX Expression System combines GIBCO® FreeStyle™ Media, FreeStyle™ MAX Reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293 cells. The only equipment required to use the FreeStyle™ MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinne...
Catalog: 16447-500
Size: 15 ml

Lipofectamine® LTX & Plus Reagent

Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents

Lipofectamine® LTX Reagent offers a new, advanced solution for gene expression studies in hard-to-transfect cells. No other plasmid DNA-specific transfection reagent can match the efficiency, convenience, and gentleness of Lipofectamine® LTX Reagent for transfection of primary, difficult-to-transfect, and sensitive cell lines. Lipofectamine® LTX Reagent is synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in experiments or cell lines. A certificate of origin may be obtained by contacting Technical Support. ...
Catalog: A12621
Size: 0.1 ml

Lipofectin® Transfection Reagent

Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents

Lipofectin® Transfection Reagent is the reagent of choice for transfectin of endothelial cells; some of our customer also use it for transfection of DNA, RNA or oligonucleotides into their cell line of choice. Lipofectin® has also been shown to work well in combination with PLUS® reagent for the transfection of HeLa cells (1).
Catalog: 18292-011
Size: 1 ml

LB Broth Base, Powder (Lennox L Broth Base)®

Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media

Formulation per one liter: 10 g SELECT Peptone 140 5 g SELECT Yeast Extract 5 g sodium chloride
Catalog: 12780-029
Size: 2.5 kg

Luria Broth Base (Miller's LB Broth Base)®, Powder

Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media

Formulation per one liter: 10 g SELECT Peptone 140 5 g SELECT Yeast Extract 10 g sodium chloride
Catalog: 12795-027
Size: 500 g

I-Block™ Protein-Based Blocking Reagent

Category: Products & Services > Buffers & Chemicals > Immunoassay Buffers & Reagents > ELISA Buffers & Reagents

This Casein-based blocking reagent can be used with Western-Light™, Western-Light Plus™, Western-Star™, ELISA-Light™, Southern-Light™, and Southern-Star™ Detection Systems. For Research Use Only. Not for use in diagnostics procedures.
Catalog: T2015
Size: 30 g

BRAF Mutation Analysis Reagents

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Genetic & Chromosome Abnormality Detection Kits

The BRAF Mutation Analysis Reagents employ a simple fragment analysis workflow to detect 1% mutant to wild type genomic DNA across 3 common and rare mutations in the BRAF gene region (see Table 1). The BRAF set provides sufficient reagent for analysis of 30 samples. Advantages of the BRAF Mutation Analysis Reagents include: • Simple Analysis—unambiguous, easy-to-interpret results • Thorough Coverage—detects 3 mutations in the BRAF gene • Sensitive—detects mutations at frequencies as low as 1 to 5% against a wild type genomic DNA background • Efficient—interrogate multiple loci from the same sa...
Catalog: 4452136
Size: 1 kit

Alexa Fluor® 488 Phalloidin

Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents

Alexa Fluor 488® phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. • Selectively stains F-actin • Outstanding fluorescence performance • Excitation/Emission: 495/518 nm • Superior to antibody staining • Optimal for fixed and permeabilized samples Get Superior Results in Your Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Flu...
Catalog: A12379
Size: 300 units

BODIPY® FL PhallAcidin

Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents

BODIPY® FL phallacidin is a high-affinity F-actin probe conjugated to our proprietary BODIPY® FL dye. The BODIPY® FL dye emits a green fluorescence similar to that of fluorescein but with a narrower emission spectra. Selectively stains F-actin Outstanding fluorescence performance Excitation/Emission: 505/512 nm Superior to antibody staining Optimal for fixed and permeabilized samples Get Superior Results in Actin Staining Studies Phallacidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging app...
Catalog: B607
Size: 300 units

Alexa Fluor® 647 Phalloidin

Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents

Alexa Fluor® 647 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, far-red fluorescent Alexa Fluor® 647 dye. Selectively stains F-actin Outstanding fluorescence performance Excitation/Emission: 650/668 nm Superior to antibody staining Optimal for fixed and permeabilized samples Get Superior Results in Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has...
Catalog: A22287
Size: 300 units

Paclitaxel, BODIPY® 564⁄570 Conjugate (BODIPY® 564⁄570 Taxol)

Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents

The red-orange-fluorescent BODIPY® 564/570 paclitaxel can be used to study microtubules in solution.
Catalog: P7501
Size: 10 µg

MitoProbe™ DiIC 1 (5) Assay Kit - For Flow Cytometry - 100 Assays

Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents

The MitoProbe™ DiIC 1 (5) Assay Kit provides solutions of the membrane-potential-sensitive cyanine dye DiIC 1 (5), and a mitochondrial membrane-potential disrupter, CCCP, for the study of mitochondrial membrane potential. DiIC 1 (5) penetrates the cytosol of eukaryotic cells and accumulates primarily in mitochondria with active membrane potentials, producing bright, far-red fluorescence. DiIC 1 (5) staining intensity decreases with decreased mitochondrial membrane potential.
Catalog: M34151
Size: 1 kit

Texas Red®-X Phalloidin

Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents

Texas Red®-X phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red fluorescent Texas Red®-X dye. Selectively stains F-actin Outstanding fluorescence performance Excitation/Emission: 591/608 nm Superior to antibody staining Optimal for fixed and permeabilized samples Get Superior Results in Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually i...
Catalog: T7471
Size: 300 units

siPORT™ Amine Transfection Agent

Category: Products & Services > Cell Culture & Transfection Reagents > Transfection Reagents

A polyamine-based Ambion® siRNA transfection agent that can be used to transfect cells during subculturing-—saving a full day of valuable time—without increasing toxicity. It is supplied in one tube containing 0.4 mL. A successful siRNA experiment requires the appropriate transfection agent for your cells, and one that is optimized for siRNA delivery. The efficiency with which different transfection reagents deliver nucleic acids can vary dramatically with the type of nucleic acid being transfected (RNA vs. DNA, single-stranded vs. double-stranded) and the cells being used. Accessory Products:...
Catalog: AM4502
Size: 400 µl