Trypan blue stain is a well-accepted method for staining dead cells. It can enter into cells with compromised membranes and is the method used with the Countess® automated cell counting platform.
Size: 2 × 1 ml
StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. Accutase® does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex™, CELLStart™ and polymers. Accutase® performs exceptionally well in detaching primary and stem cells and maintaining high cell viabilty compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase® treatment include analysis of cell su...
Size: 100 ml
StemPro® CD34+ cells are pooled Human Hematopoietic Progenitor cells (HPCs) derived from the umbilical cord blood of mixed donors. Along with 0.5×10 6 pooled donor cord blood-derived CD34+ cells, this kit includes StemPro®-34 SFM basal liquid medium and frozen StemPro®-34 Nutrient Supplement to facilitate the immediate culturing of the StemPro® CD34+ cells upon arrival or when recovering from cryopreservation storage. With the StemPro® CD34+ Cell Kit You Get: • ≥90% purity of the desired CD34+ cell population • Each vial of cells contains 0.5×10 6 CD34+ cells with full traceability • StemPro®-...
Size: 0.5 x 10
Mouse Embryonic stem cells (ESCs) were isolated from mouse (Strain C57BL⁄6) embryos at Day E3.5. Before cryopreservation, the cells were tested for viability (>70% post-thaw viability), their ability to remain undifferentiated when cultured on mouse feeder cells as assayed by marker expression (Oct4, SSEA-1, Nanog) and their ability to differentiate into embryoid bodies and express markers indicative of three germ layers (Ectoderm: (+) for β3-tubulin and nestin, Mesoderm: (+) for smooth muscle actin, Endoderm: (+) for AFP). Cells were cryopreserved at passage 20 (P20). For optimal growth perfo...
Size: 1 ml
The Violet Ratiometric Membrane Asymmetry Probe⁄Dead Cell Apoptosis Kit provides a simple and fast method for the detection of apoptosis with dead cell discrimination by flow cytometry. The Violet Ratiometric Membrane Asymmetry Probe, 4'-N,N-diethylamino-6-(N,N,N-dodecyl-methylamino-sulfopropyl)-methyl-3-hydroxyflavone (F2N12S), is a novel violet excitable dye for the detection of membrane asymmetry changes during apoptosis. The dye exhibits an excited-state intramolecular proton transfer (ESIPT) reaction resulting in a dual fluorescence with two emission bands corresponding to 530 nm and 585 ...
Size: 1 kit
MAX Efficiency® Stbl2™ Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts. In addition to rec A1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences (1) and tandem array genes (2). MAX Efficiency® Stbl2™ Competent Cells offer: >1 x 10 9 transformants/flg for cloning rare sequences Elimination of mcr BC- hsd RMS- mrr and mutation of mcr A allows cloning of methylated genomic sequences (1) Unique genotype stabilizes direct repeat and retroviral sequences such as SIV and HIV (1) Greatly ...
Size: 1 ml
For Academic pricing please login or contact us at email@example.com. The Tango™ CCR5- bla U2OS cells contain the human Chemokine (C-C Motif) Receptor 5 (CCR5) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR- bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase ( bla ) reporter gene under the control of a UAS response element. The Tango™ CCR5- bla U2OS cells are functionally validated for Z' and EC 50 concentrations of MIP-1a. In addition,...
Size: 8 × 10^6 cells
The ER-Tracker™ Red dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained using the protocol provided, the staining pattern is partially retained after fixation with formaldehyde. This stain consists of the green-fluorescent BODIPY® TR dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K + channels which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell typ...
Size: 100 µg
MAX Efficiency® DH10Bac™ Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac® Baculovirus Expression System . The DH10Bac™ E. coli strain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac™, to create an expression bacmid containing a cloned gene of interest. Features of MAX Efficiency® DH10Bac™ Competent Cells include: • Consistent yields of >1 × 10 8 transformants/ µg • Tolerance of small amounts of undiluted ligation reactions • Support of efficient blue/white colony screening (Φ80 lac ZΔM15 marker) About MAX Ef...
Size: 5 × 100 µl
The same lysis buffer found in the Ambion® Cells-to-Signal™ Kit. Supplied in 5 bottles containing 10 mL each.
Size: 5 bottle
Gibco® Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS). Gibco® Cell Dissociation Buffer is suitable for the gentle dissociation of mammalian cells for studies such as ligand binding, flow cytometry, and immunohistochemistry, that require intact cell surface proteins. Gibco® Cell Dissociation Buffer works well with lightly adherent cell lines, such as HeLa and NIH 3T3, but is not recommended for the routine passaging of strongly adherent...
Size: 100 ml
One Shot® ccd B Survival™ 2 T1 Phage-Resistant (T1 R ) chemically competent E. coli are suitable for the propagation of plasmids containing the ccd B gene, and are designed for use with the Gateway® Vector Conversion System and for propagating Gateway® destination, donor, and super-coiled entry vectors. Transformation efficiency is >1 × 10 9 transformants/ µg DNA. The ccd B Survival™ 2 T1 R E. coli strain is derived from the TOP10 strain. The ccdB Survival™ 2 T1 R E. coli strain offers the following Features: • Resistance to the ccd B gene product • Resistance to T1 and T5 phage (the ton A/ fh...
Size: 10 transformations