Baculovirus Kits

pMIB/V5-His Vector Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Insect & Baculovirus Expression Vectors

pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five™ insect cells. Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells. pMIB/V5-His has several features to facilitate expression of recombinant proteins in insect cells including: The OpIE 2 promoter for constitutive expression The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein The Blasticidin resistance gene for rapid selection of stably transfected c...

Catalog: V8030-01
Size: 1 kit

BaculoDirect™ C-Term Expression Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Insect & Baculovirus Expression Vectors

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...

Catalog: 12562-013
Size: 5 transfections

pIZT/V5-His Vector Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Insect & Baculovirus Expression Vectors

The InsectSelect™ Glow Kit features the pIZT/V5-His vector for high-level expression of your gene of interest. This vector has the following features: The OpIE 2 promoter for constitutive expression The Zeocin™ resistance gene for rapid selection of stably transfected cell lines C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistance protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid...

Catalog: V8010-01
Size: 1 kit

DES® TOPO® TA Expression Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Insect & Baculovirus Expression Vectors

The DES® TOPO® TA Expression Kit offers one-step cloning of PCR products directly into an inducible DES® expression vector. The kit uses the linearized, topoisomerase I-activated pMT/V5-His-TOPO® vector for fast and easy cloning and subsequent high-level expression. Topoisomerase activation of this vector allows PCR products to be ligated in just 5 minutes on your bench top to produce >85%recombinants. The pMT/V5-His-TOPO® vector has all of the features of the pMT/V5-His vector to simplify protein expression, detection, and purification of recombinant proteins in Drosophila S2 cells.

Catalog: K4125-01
Size: 20 reactions