Avidin Agarose

UltraPure™ Agarose

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Agarose

UltraPure™ Agarose is a polysaccharide (see structure below) used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Agarose is ideal for resolving DNA and RNA fragments from 500 bp to > 20 kb. • Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handling and less breakage • Can be used in protein electrophoresis applications such as Ouchterlony (antigen-antibody interaction assay) and radial immunodiffusion (RID) (antigen quantitation assay) Improved Packaging The new environmentally ...
Catalog: 16500100
Size: 100 g

Avidin, NeutrAvidin®, Horseradish Peroxidase Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Neutravidin

Our horseradish peroxidase (HRP) conjugate of NeutrAvidin® biotin-binding protein - a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point - can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding.
Catalog: A-2664
Size: 1 mg

Avidin - HRP

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Avidin

Reactivity: HRP-Avidin reacts with biotin. Applications: Immunohistochemistry, ELISA
Catalog: 43-4423
Size: 5 mg

UltraPure™ Low Melting Point Agarose

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Agarose

UltraPure™ Low Melting Point Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Low Melting Point Agarose is ideal for resolving DNA and RNA fragments larger than 1,000 bp and for the recovery of nucleic acid fragments after electrophoresis, as it melts at 65.5°C (below the melting point of most nucleic acids). The solution remains fluid at 37°C and will set rapidly at temperatures below 25°C. • Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handlin...
Catalog: 16520100
Size: 100 g

DNA Grade Agarose

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Agarose

DNA Grade Agarose has a high sieve capacity, providing optimal resolution of DNA during gel electrophoresis. The low sulfur content allows gels to be run faster without becoming brittle. Supplied as a powder that is reconstituted in 0.5X TBE for high-quality agarose gels.
Catalog: 75000-500
Size: 500 g

UltraPure™ Agarose

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Agarose

UltraPure™ Agarose is a polysaccharide (see structure below) used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Agarose is ideal for resolving DNA and RNA fragments from 500 bp to > 20 kb. • Ideal for analysis and recovery of DNA and RNA for routine applications • Strong gel structure allows for better handling and less breakage • Can be used in protein electrophoresis applications such as Ouchterlony (antigen-antibody interaction assay) and radial immunodiffusion (RID) (antigen quantitation assay) Improved Packaging The new environmentally ...
Catalog: 16500500
Size: 500 g

pHrodo™ Red Avidin (Fluorogenic pH Sensor)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Avidin

pHrodo™ Red Avidin is a conjugate of avidin and pHrodo™ Red dye, a novel, fluorogenic dye that dramatically increases in fluorescence as the pH of its surroundings become more acidic. pHrodo™ Red dye conjugates are non-fluorescent outside the cell, but fluoresce bright red in phagosomes, making them ideal tools for studies ranging from phagocytosis of bioparticles to receptor internalization. Original pHrodo™ and pHrodo™ Red dye are the same molecule. With pHrodo™ Red Avidin Fluorogenic pH Sensor You Get: • Specific detection of phagocytosis and endocytosis • Faster staining • More accurate re...
Catalog: P35362
Size: 1 mg

4% Agarose

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Agarose

Gibco® 4% Agarose is an autoclaved, high purity, low melting point gel designed for plaque assays and the purification of baculovirus. The 4% Agarose Gel melts at 65 – 70°C and remains fluid at 37°C. When diluted with concentrated insect cell culture medium, Gibco® 4% Agarose produces a gel firm enough to immobilize local infections, yet ductile enough to allow for the efficient removal of plaques for clonal expansion. Product Use For Research Use Only. Not for use in diagnostic procedures.
Catalog: 18300-012
Size: 40 ml

Avidin, NeutrAvidin® Biotin-binding Protein

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Neutravidin

Our NeutrAvidin® biotin-binding protein - a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point - can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding.
Catalog: A-2666
Size: 5 mg

Avidin, NeutrAvidin®, Fluorescein Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Neutravidin

Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
Catalog: A-2662
Size: 1 mg

Avidin - FITC

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Avidin

Reactivity: FITC-Avidin reacts with biotin.
Catalog: 43-4411
Size: 5 mg

Goat Anti-Mouse IgG (H+L) agarose

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Affinity purified whole antibody goat anti-mouse IgG conjugated to 4% beaded, crosslinked agarose (Sepharose® CL-4B). To minimize cross-reactivity, this antibody has been absorbed against human IgG and human serum.
Catalog: A10516
Size: 2 ml

Glutathione Agarose, Linked Through Sulfur, sedimented bead suspension

Category: Products & Services > Columns, Resins & Plates > Chromatographic Columns & Media > Affinity Media & Standards

Glutathione agarose prepared by linking the sulfur atom of glutathione to crosslinked beaded agarose can be used for purification of GST fusion proteins. Each milliliter of gel can bind approximately 5-6 mg of boivine-liver GST. Adding excess free glutathione liberates the GST fragment from the matrix which can then be regenerated by washing with a high-salt buffer.
Catalog: G2879
Size: 10 ml

Avidin/Biotin Blocking Kit

Category: Products & Services > Buffers & Chemicals > Microscopy Reagents

This kit is used to block endogenous biotin or biotin-binding activity in tissue sections. The kit contains 18 mL each of Avidin and Biotin solutions.
Catalog: 00-4303
Size: 1 kit

Streptavidin, Marina Blue® conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin

Our streptavidin, Marina Blue™ conjugate is a bright, blue-fluorescent probe with excitation/emission ~365/460 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.
Catalog: S-11221
Size: 1 mg

CaptAvidin™ Biotin-Binding Protein

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Avidin

CaptAvidin™ biotin-binding protein contains a nitrated tyrosine in its biotin-binding site. This chemical modification permits dissociation of the avidin-biotin complex under milder conditions than the traditional avidin-biotin complex. At pH 4.0, CaptAvidin™ biotin-binding protein binds biotin tightly with a K a of ~10 9 M -1 . This association can then be reversed at pH 10, allowing complete dissociation of the avidin-biotin complex. This is in contrast to the harsh conditions (8 M guanidine hydrochloride, pH 1.5) needed to dissociate the avidin-biotin complex.
Catalog: C-21385
Size: 1 mg

Pippin Prep™ Kit 3010

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Nucleic Acid Gels > Agarose Gels

The Pippin Prep™ Kit 2010 contains 10 disposable pre-cast 3% agarose gel cassettes and a reagent pack for use isolating DNA fragments in the 50 - 300 bp range. The gel cassettes have 5 lanes, allowing 4 samples to be run along with an external size standard. The reagent pack includes that external standard as well as loading solution and electrophoresis buffer. Pippen Prep™ Kits can be purchased separately and are available in three different formats. Streamlined Pippin Prep™ System DNA Size Selection Process The workflow for Pippin Prep™ DNA size selection consists of just a few simple steps:...
Catalog: 4472172
Size: 10 reactions

Streptavidin - FITC

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin

Reactivity: FITC-SA will react with biotin.
Catalog: 43-4311
Size: 1 mg

Streptavidin-Fluorescein Isothiocyanate (FITC) conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin

The pcDNA™6 BioEase™ Gateway® Biotinylation System provides an easy, efficient method for expressing, purifying, and detecting biotinylated recombinant proteins. The pcDNA™6/BioEase™-DEST vector (Figure 1) incorporates a 72 amino acid sequence from K. pneumoniae that directs in vivo biotinylation of a specific lysine residue. This K. pneumoniae tag is recognized and efficiently biotinylated in vivo by a native mammalian system similar to the enzyme Biotin Protein Ligase (BPL) in E. coli (1). Proteins produced from the pcDNA™6/BioEase™-DEST vector are expressed as fusion proteins to this sequen...
Catalog: SA100-02
Size: 50 µl

Streptavidin-Horseradish Peroxidase (HRP) conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin

Streptavidin-Horseradish Peroxidase (HRP) conjugate
Catalog: SA100-01
Size: 50 µl