Attune Reactive Oxygen & Nitrogen Species
SNAP spontaneously releases nitric oxide (NO) under physiological conditions.
Catalog: N-7892
Size: 25 mg
Size: 25 mg
DAF-FM is a reagent that is used to detect and quantify low concentrations of nitric oxide (NO). It is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. DAF-FM fluorescence can be detected by any instrument that can detect fluorescein, including flow cytometers, microscopes, fluorescent microplate readers and fluorometers. DAF-FM Specifications: • Ex/Em of DAF-FM: ~495/515 nm • Lyophilized product should be dissolved using DMSO and then added to an aqueous buffer to create a working solution • DAF-FM diacetate is cell permeant and passively diffuses across...
Catalog: D-23844
Size: 10 × 50 µg
Size: 10 × 50 µg
MTT is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble MTT to an insoluble purple formazan. The formazan is then solubilized and its concentration determined by optical density.
Catalog: M-6494
Size: 1 g
Size: 1 g
CM-H 2 DCFDA is a chloromethyl derivative of H 2 DCFDA, useful as an indicator for reactive oxygen species (ROS) in cells. This indicator exhibits much better retention in live cells than H 2 DCFDA. CM-H 2 DCFDA passively diffuses into cells, where its acetate groups are cleaved by intracellular esterases and its thiol-reactive chloromethyl group reacts with intracellular glutathione and other thiols. Subsequent oxidation yields a fluorescent adduct that is trapped inside the cell, thus facilitating long-term studies. ROS Indicator Specifications: • Ex/Em: ~492–495/517–527 nm • Product is air ...
Catalog: C6827
Size: 20 × 50 µg
Size: 20 × 50 µg
OxyBURST® Green H 2 DCFDA succinimidyl ester (2',7'-dichlorodihydrofluorescein diacetate, SE) is an amine-reactive assay reagent that can be used to prepare oxidation-sensitive conjugates of a wide variety of biomolecules and particles, including antibodies, antigens, peptides, proteins, dextrans, bacteria, yeast and polystyrene microspheres. Following conjugation to amines, the two acetates of OxyBURST® Green H 2 DCFDA can be removed by treatment with hydroxylamine at neutral pH to yield the dihydrofluorescein conjugate. OxyBURST® Green H 2 DCFDA conjugates are nonfluorescent until they are o...
Catalog: D-2935
Size: 5 mg
Size: 5 mg
MitoSOX™ Red reagent is a novel fluorogenic dye specifically targeted to mitochondria in live cells. Oxidation of MitoSOX™ Red reagent by superoxide produces red fluorescence. • Readily oxidized by superoxide but not by other ROS- or RNS-generating systems • Absorption/emission maxima: ~510/580 nm • Use for live cell imaging • Rapidly and selectively targeted to the mitochondria Quick, Easy Detection of Mitochondrial Superoxide in Live Cells The production of superoxide by mitochondria can be visualized in fluorescence microscopy using the MitoSOX™ Red reagent. MitoSOX™ Red reagent permeates l...
Catalog: M36008
Size: 10 × 50 µg
Size: 10 × 50 µg
The Attune® Computer Upgrade Kit allows the Attune® Acoustic Focusing Cytometer to operate the Attune® Cytometric Software v2.1. This software is required for use of the Attune® Auto Sampler, which enables rapid processing of multiple samples by automatically analyzing samples from 96- or 384-well standard and deep-well plates. The Attune® Computer Upgrade Kit includes a Dell® 64-bit computer with Windows® 7 operating system, Attune® Cytometric Software v2.1, and v2.1 documentation, as well as a visit by a trained service technician to assist with data transfer from the existing computer syste...
Catalog: 4481653
Size: 1 kit
Size: 1 kit
This 96-well plate is used on the Attune® Auto Sampler. It is a non-treated polystyrene plate with U-bottom wells and a low-evaporation lid. Features include: • Bacteriological-grade polystyrene that is non-treated for a more hydrophobic growth surface and reduced cell attachment • Alphanumeric well identification • Condensation rings • Lid for reduced evaporation and contamination • Individually packaged in convenient peel-open medical-style packaging (recyclable PET trays) • Sterilized by gamma irradiation, nonpyrogenic
Catalog: 4477131
Size: 1 each
Size: 1 each
The thiol-reactive Alexa Fluor 546 C 5 maleimide and its conjugates are perfect for applications that require orange-fluorescent probes. Spectrally similar to the Cy3 dye, the Alexa Fluor 546 dye is readily excited by the 546 nm line of the mercury-arc lamp and the 543 nm line of the He-Ne laser.
Catalog: A-10258
Size: 1 mg
Size: 1 mg
The amine-reactive Lissamine™ rhodamine B sulfonyl chloride can be used to create red-fluorescent bioconjugates with excitation/emission maxima ~568/583 nm.
Catalog: L-20
Size: 1 g
Size: 1 g
Our 4-acetamido-4'-maleimidylstilbene-2,2'- disulfonic acid is a thiol-reactive reagent that is water soluble, with high polarity and membrane impermeability. This polysulfonated dye is useful for determining whether thiol-containing proteins and polypeptide chains are exposed at the extracellular or cytoplasmic membrane surface.
Catalog: A-485
Size: 25 mg
Size: 25 mg
The red-fluorescent tetramethylrhodamine (TAMRA) alkyne can be reacted with azides via a copper-catalyzed click reaction. Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moities to label and detect a molecule of interest using a two-step procedure. The two-step reaction procedure involves a copper-catalyzed triazole formation of an azide and an alkyne. Click reactions have several characteristics: the reaction between the detection moieties is efficient; no extreme temperatures or solvents are required; the reaction product is stable; the ...
Catalog: T10183
Size: 0.5 mg
Size: 0.5 mg
The Alexa Fluor 488 hydrazide is a low molecular weight, cell membrane—impermeant, aldehyde-fixable molecule that can be used as a cell tracer. This green-fluorescent polar dye can be loaded into cells by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent (I-14402).
Catalog: A-10436
Size: 1 mg
Size: 1 mg
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
Catalog: F-150
Size: 25 mg
Size: 25 mg
DTNB or Elman's reagent can be used to quantitate thiols in proteins, cells and plasma by absorption measurements. It readily forms a mixed disulfide with thiols, liberating the chromophore 5-merapto-2-nitrobenzoic acid (absorption maximum 410 nm). Only protein thiols that are accessible to this water-soluble reagent are modified.
Catalog: D-8451
Size: 10 g
Size: 10 g
The phycobiliprotein, allophycocyanin (APC) can be conjugated to proteins such as antibodies and other molecules to produce conjugates with excitation/emission maxima ~650/680 for use in flow cytometry applications. The Protein-Protein Crosslinking Kit (P6305) is optimized to crosslink APC to IgG antibodies.
Catalog: A-803
Size: 0.5 ml
Size: 0.5 ml
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
Catalog: C-6164
Size: 10 mg
Size: 10 mg
Tetrafluorophenyl (TFP) esters are an improvement over the succinimidyl ester (SE or NHS-ester) chemistry typically used to attach fluorophores or haptens to the primary amines of biomolecules. Both reactive chemistries produce the same strong amide bond between the dye or hapten and the compound of interest, but TFP esters are less susceptible to spontaneous hydrolysis during conjugation reactions. Alexa Fluor TFP esters are stable for several hours at the basic pH typically used for reactions – far outlasting succinimidyl esters.
Catalog: A-30005
Size: 1 mg
Size: 1 mg
Conjugates prepared with the thiol-reactive Alexa Fluor 647® C 2 maleimide are spectrally similar to the Cy5 dye, resulting in an optimal match to optical filters for the Cy5 dye. Total fluorescence of secondary antibody conjugates of Alexa Fluor® 647 dye, however, is significantly higher than that of Cy5 conjugates. Unlike the Cy5 dye, Alexa Fluor® 647 dye has very little change in absorption of fluorescence spectra when conjugated to most porteins, oligonucleotides, or nucleic acids, yielding greater total fluorescence at the same degree of substitution.
Catalog: A-20347
Size: 1 mg
Size: 1 mg
Conjugates prepared with the thiol-reactive Alexa Fluor 488 C 5 maleimide are significantly brighter and more photostable than fluorescein. The bright, green fluorescence of the Alexa Fluor 488 dye, which is spectrally similar to that of fluorescein, is pH independent from pH 4 to 10.
Catalog: A-10254
Size: 1 mg
Size: 1 mg
Attune Reactive Oxygen & Nitrogen Species
Previous: attune instruments | Next: Attune Software
View products by:
Title |
Brand |
Price |
Popularity |
Best Sellers
View topsellers by:
Title |
Brand |
Price |
Popularity |
Favourites
Popular items by:
Popularity |
Our Choices |
All-Round Favourites |
Title |
Popularity |
Our Choices |
All-Round Favourites |
Title
Previous: attune instruments | Next: Attune Software
