Attune Primary Cells
PLUS™ Reagent is used in conjunction with transfection reagents, such as Lipofectamine™, to enhance transfection in adherent cell lines. Some customers find when using Plus™ Reagent that less optimization of DNA concentration is needed to achieve high transfection performance. The addition of Plus™ Reagent may also reduce the quantities of DNA and lipid required by approximately one-half compared to lipid alone. PLUS™ Reagent was specifically designed to expand the array of transfectable cell types (1). PLUS™ Reagent used with Lipofectamine™ Reagent has been demonstrated to improve the transfe...
Catalog: 11514-015
Size: 0.85 ml
Size: 0.85 ml
Lipofectamine® RNAiMAX Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments. Lipofectamine® RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically for the delivery of siRNA and miRNA into all cell types. With Lipofectamine® RNAiMAX Transfection Reagent you will get: Superior transfection efficiency requiring lower RNAi concentrations leading to more effective gene knockdown with minimal non-specific effects Easy optimization due to minimal cytotoxicity across a 10-f...
Catalog: 13778-100
Size: 0.1 ml
Size: 0.1 ml
Lipofectamine® LTX Reagent offers a new, advanced solution for gene expression studies in hard-to-transfect cells. No other plasmid DNA-specific transfection reagent can match the efficiency, convenience, and gentleness of Lipofectamine® LTX Reagent for transfection of primary, difficult-to-transfect, and sensitive cell lines. Lipofectamine® LTX Reagent is synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in experiments or cell lines. A certificate of origin may be obtained by contacting Technical Support. ...
Catalog: A12621
Size: 0.1 ml
Size: 0.1 ml
Lipofectin® Transfection Reagent is the reagent of choice for transfectin of endothelial cells; some of our customer also use it for transfection of DNA, RNA or oligonucleotides into their cell line of choice. Lipofectin® has also been shown to work well in combination with PLUS® reagent for the transfection of HeLa cells (1).
Catalog: 18292-011
Size: 1 ml
Size: 1 ml
A polyamine-based Ambion® siRNA transfection agent that can be used to transfect cells during subculturing-—saving a full day of valuable time—without increasing toxicity. It is supplied in one tube containing 0.4 mL. A successful siRNA experiment requires the appropriate transfection agent for your cells, and one that is optimized for siRNA delivery. The efficiency with which different transfection reagents deliver nucleic acids can vary dramatically with the type of nucleic acid being transfected (RNA vs. DNA, single-stranded vs. double-stranded) and the cells being used. Accessory Products:...
Catalog: AM4502
Size: 400 µl
Size: 400 µl
GIBCO® Astrocyte Medium has been specially formulated to support the growth of primary human and rat astrocytes while retaining their phenotypes. The medium has been internally validated and optimized to provide an optimal balance between cell growth and the maintenance of cell viability, normal phenotype, and function. If more rapid proliferation is desired, addition of an appropriate amount of epidermal growth factor (EGF) is recommended, although cells may exhibit morphological and phenotypic changes. Astrocytes are a type of glial cells of the nervous system with a typical star shape. It h...
Catalog: A12613-01
Size: 500 ml
Size: 500 ml
Cryopreserved human hepatocytes for use in transporter-mediated uptake applications. Cells per vial: 4-8 million Prototypical hepatic uptake transporter substrates taurocholate and estradiol-17β-glucuronide have been assessed to qualify the transport function of hepatic NTCP (SLC10A1) and OATP1B14/3 (SLCO1B1/3), respectively. The transport of each substrate (radiolabeled) was monitored by scintillation analysis and active transport values (37°C - 4°C) determined by linear regression. Get the Results You Need with GIBCO® Hepatocytes High quality hepatocytes, with high viabilities, in vivo -like...
Catalog: HMCPUS
Size: 1.5 ml
Size: 1.5 ml
The F4/80, clone BM8, Pacific Blue™ dye conjugate is a monoclonal rat antibody that is specific to mouse F4/80 and recognizes the expressed product of the Emr1 gene. Our Pacific Blue™ dye is optimally excited by the violet laser and conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis. Antibody Specifications Description: F4/80, Rat IgG2a monoclonal Label: Pacific Blue™ dye Validated Application: Flow Cytometry Spec...
Catalog: MF48028
Size: 1 ml
Size: 1 ml
The CD90/Thy-1 (clone G7) R-PE (phycoerythrin) conjugate is a monoclonal rat antibody that recognizes the Thy-1.1 and Thy-1.2 alloantigens (Thy-1 epitope region A). R-PE is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thereby affording exceptional quantum yields and molar extinction coefficients. The use of PE in antibody conjugates provides probes with greatly enhanced detectability. Antibody Specifications Description: CD90/Thy-1, Rat IgG 2c monoclonal Label: R-PE (phycoer...
Catalog: A14726
Size: 25 µg
Size: 25 µg
The CD2, clone S5.5, Qdot® 705 nanocrystal conjugate is a mouse monoclonal antibody (mAb) that recognizes the CD2 antigen. The Qdot® 705 nanocrystal conjugate may be used in the same way as conventional antibody conjugates and is most often used with 405 nm excitation, permitting simultaneous multicolor labeling of multiple targets off the violet laser. Alternatively, the nanocrystal can be excited by any laser up to the wavelength at which it absorbs. Antibody Specifications Description: CD2, mouse IgG2a monoclonal Label: Qdot® 705 nanocrystal Validated Application: Flow Cytometry Species Rea...
Catalog: Q22141
Size: 25 µL
Size: 25 µL
The CD4, clone RM4-5, Qdot® 655 nanocrystal conjugate is a rat monoclonal antibody (mAb) (mAb) that recognizes the mouse CD4 antigen. The Qdot® 655 nanocrystal conjugate may be used in the same way as conventional antibody conjugates and is most often used with 405 nm excitation, permitting simultaneous multicolor labeling of multiple targets off the violet laser. Alternatively, the nanocrystal can be excited by any laser up to the wavelength at which it absorbs. Antibody Specifications Description: CD4, rat IgG2a monoclonal Label: Qdot® 655 nanocrystal Validated Application: Flow Cytometry Sp...
Catalog: Q22163
Size: 25 µL
Size: 25 µL
The CD4, clone RM4-5, Qdot® 800 nanocrystal conjugate is a rat monoclonal antibody (mAb) (mAb) that recognizes the mouse CD4 antigen. The Qdot® 800 nanocrystal conjugate may be used in the same way as conventional antibody conjugates and is most often used with 405 nm excitation, permitting simultaneous multicolor labeling of multiple targets off the violet laser. Alternatively, the nanocrystal can be excited by any laser up to the wavelength at which it absorbs. Antibody Specifications Description: CD4, rat IgG2a monoclonal Label: Qdot® 800 nanocrystal Validated Application: Flow Cytometry Sp...
Catalog: Q22165
Size: 25 µL
Size: 25 µL
The CD19, clone SJ25-C1, Qdot® 800 nanocrystal conjugate is a mouse monoclonal antibody (mAb) (mAb)that recognizes the CD19 antigen. The Qdot® 800 nanocrystal conjugate may be used in the same way as conventional antibody conjugates and is most often used with 405 nm excitation, permitting simultaneous multicolor labeling of multiple targets off the violet laser. Alternatively, the nanocrystal can be excited by any laser up to the wavelength at which it absorbs. Antibody Specifications Description: CD19, mouse IgG1 monoclonal Label: Qdot® 800 nanocrystal Validated Application: Flow Cytometry S...
Catalog: Q22139
Size: 25 µL
Size: 25 µL
The CD19, clone 6D5, Qdot® 705 nanocrystal conjugate is a rat monoclonal antibody (mAb) that reacts with mouse CD19 and recognizes the expressed product of the mouse CD19 gene. The Qdot® 705 nanocrystal conjugate may be used in the same way as conventional antibody conjugates and is most often used with 405 nm excitation, permitting simultaneous multicolor labeling of multiple targets off the violet laser. Alternatively, the nanocrystal can be excited by any laser up to the wavelength at which it absorbs. Antibody Specifications Description: CD19, rat IgG2a monoclonal Label: Qdot® 705 nanocrys...
Catalog: Q22161
Size: 25 µL
Size: 25 µL
CD45R (B220), clone RA3-6B2, Qdot® 705 nanocrystal conjugate is a rat monoclonal antibody (mAb) that recognizes the expressed product of the PTPRC gene. The Qdot® 705 nanocrystal conjugate may be used in the same way as conventional antibody conjugates and is most often used with 405 nm excitation, permitting simultaneous multicolor labeling of multiple targets off the violet laser. Alternatively, the nanocrystal can be excited by any laser up to the wavelength at which it absorbs. Antibody Specifications Description: CD45R (B220), rat IgG2a monoclonal Label: Qdot® 705 nanocrystal Validated Ap...
Catalog: Q22167
Size: 25 µL
Size: 25 µL
The CD45, clone HI30, PerCP-Cy ® 5.5 conjugate is a mouse monoclonal antibody (mAb) that recognizes human CD45 molecule. The PerCP-Cy ® 5.5 tandem conjugate combines the peridinin-chlorophyll proteins (PerCP) complexes, found in nature, with the Cy ® 5.5 cyanine dye; thereby permitting simultaneous multicolor labeling and detection of multiple targets by utilizing a single excitation source - the 488 nm line of an argon-ion laser. Antibody Specifications Description: CD45, Mouse IgG 1 monoclonal Label: PerCP-Cy ® 5.5 tandem dye Validated Application: Flow Cytometry Species Reactivity: Human Ex...
Catalog: A14796
Size: 25 µg
Size: 25 µg
The CD2, clone S5.5, Qdot® 655 nanocrystal conjugate is a mouse monoclonal antibody (mAb) that recognizes the CD2 antigen. The Qdot® 655 nanocrystal conjugate may be used in the same way as conventional antibody conjugates and is most often used with 405 nm excitation, permitting simultaneous multicolor labeling of multiple targets off the violet laser. Alternatively, the nanocrystal can be excited by any laser up to the wavelength at which it absorbs. Antibody Specifications Description: CD2, mouse IgG2a monoclonal Label: Qdot® 655 nanocrystal Validated Application: Flow Cytometry Species Rea...
Catalog: Q22140
Size: 25 µL
Size: 25 µL
The CD2, clone S5.5, Qdot® 800 nanocrystal conjugate is a mouse monoclonal antibody (mAb) that recognizes the CD2 antigen. The Qdot® 800 nanocrystal conjugate may be used in the same way as conventional antibody conjugates and is most often used with 405 nm excitation, permitting simultaneous multicolor labeling of multiple targets off the violet laser. Alternatively, the nanocrystal can be excited by any laser up to the wavelength at which it absorbs. Antibody Specifications Description: CD2, mouse IgG2a monoclonal Label: Qdot® 800 nanocrystal Validated Application: Flow Cytometry Species Rea...
Catalog: Q22142
Size: 25 µL
Size: 25 µL
CD45R (B220), clone RA3-6B2, Qdot® 800 nanocrystal conjugate is a rat monoclonal antibody (mAb) that recognizes the expressed product of the PTPRC gene. The Qdot® 800 nanocrystal conjugate may be used in the same way as conventional antibody conjugates and is most often used with 405 nm excitation, permitting simultaneous multicolor labeling of multiple targets off the violet laser. Alternatively, the nanocrystal can be excited by any laser up to the wavelength at which it absorbs. Antibody Specifications Description: CD45R (B220), Rat IgG2a monoclonal Label: Qdot® 800 nanocrystal Validated Ap...
Catalog: Q22168
Size: 25 µL
Size: 25 µL
The CD4, clone RM4-5, Qdot® 705 nanocrystal conjugate is a rat monoclonal antibody (mAb) (mAb) that recognizes the mouse CD4 antigen. The Qdot® 705 nanocrystal conjugate may be used in the same way as conventional antibody conjugates and is most often used with 405 nm excitation, permitting simultaneous multicolor labeling of multiple targets off the violet laser. Alternatively, the nanocrystal can be excited by any laser up to the wavelength at which it absorbs. Antibody Specifications Description: CD4, rat IgG2a monoclonal Label: Qdot® 705 nanocrystal Validated Application: Flow Cytometry Sp...
Catalog: Q22164
Size: 25 µL
Size: 25 µL
Attune Primary Cells
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