Attune Insulin, Transferrin & Iron Supplements
Gibco® Insulin, Transferrin, Selenium, Sodium Pyruvate Solution (ITS -A) is used as a basal medium supplement in order to reduce the amount of Fetal Bovine Serum (FBS) used to culture the cells. Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G is now routinely used with other basal media to support a wide variety of cell types in the presence of 2-4% FBS. Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and it may also help to reduce toxic levels of oxyge...
Catalog: 51300-044
Size: 10 ml
Size: 10 ml
Gibco® HAT Supplement is a liquid mixture of sodium hypoxanthine (5 mM), aminopterin (20 µM) and thymidine (0.8 mM). HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT–myeloma cells. Hypoxanthine and thymidine supply preformed purines and pyrimidines for DNA synthesis by hybridomas via the salvage pathway that utilizes HGPRT- contributed by the fused spleen cell. Aminopterin, a folic acid antagonist, inhibits the de novo nucleoside biosynthesis pathway. Product Use For Research Use Only. Not for use in diagnostic procedures. Dual site cGMP Manufac...
Catalog: 21060-017
Size: 100 ml
Size: 100 ml
A sterile set of solutions intended for use with EpiLife® medium or Medium 154 to culture human epidermal keratinocytes. The HKGS Kit provides, in separate vials, all the components of complete HKGS (S-001-5). A vial of gentamycin/amphotericin solution (GA) is also included. Use of GA is optional.
Catalog: S-001-K
Size: 1 kit
Size: 1 kit
N-2 Supplement is a chemically defined, serum-free supplement based on Bottenstein’s N-1 formulation. N-2 Supplement is intended for use with Neurobasal® media. N-2 Supplement is recommended for growth and expression of neuroblastomas as well as post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS). N-2 Supplement can be used as a substitute for Bottenstein’s N-1 formulation. N-2 Supplement is provided as a 100X concentrate. N-2 Supplement may be used with Neurobasal® media supplemented with growth factors such as bFGF and E...
Catalog: 17502-048
Size: 5 ml
Size: 5 ml
A defined, sterile, concentrated (100X) solution intended for use with EpiLife® medium to culture human epidermal keratinocytes. EDGS is not intended for use with Medium 154. EDGS contains all of the growth factors and hormones necessary for the growth of human epidermal keratinocytes. Each bottle contains the correct amount of supplement for a 500 ml bottle of EpiLife® medium. EDGS contains: • purified bovine serum albumin • purified bovine transferrin • hydrocortisone • recombinant human insulin-like growth factor type-1 (rhIGF-1) • prostaglandin E2 (PGE2) • recombinant human epidermal growt...
Catalog: S-012-5
Size: 5 ml
Size: 5 ml
The Attune® Computer Upgrade Kit allows the Attune® Acoustic Focusing Cytometer to operate the Attune® Cytometric Software v2.1. This software is required for use of the Attune® Auto Sampler, which enables rapid processing of multiple samples by automatically analyzing samples from 96- or 384-well standard and deep-well plates. The Attune® Computer Upgrade Kit includes a Dell® 64-bit computer with Windows® 7 operating system, Attune® Cytometric Software v2.1, and v2.1 documentation, as well as a visit by a trained service technician to assist with data transfer from the existing computer syste...
Catalog: 4481653
Size: 1 kit
Size: 1 kit
This 96-well plate is used on the Attune® Auto Sampler. It is a non-treated polystyrene plate with U-bottom wells and a low-evaporation lid. Features include: • Bacteriological-grade polystyrene that is non-treated for a more hydrophobic growth surface and reduced cell attachment • Alphanumeric well identification • Condensation rings • Lid for reduced evaporation and contamination • Individually packaged in convenient peel-open medical-style packaging (recyclable PET trays) • Sterilized by gamma irradiation, nonpyrogenic
Catalog: 4477131
Size: 1 each
Size: 1 each
RHU INSULIN
Catalog: 0045077II
Size: 1 each
Size: 1 each
This is a purified rabbit anti-human Insulin Receptor Polyclonal Antibody, that can be used in the validated applications of Western blot, ELISA, and immunohistochemistry. This Insulin Receptor Polyclonal Antibody was produced in rabbits immunized with purified, recombinant human Insulin Receptor. Insulin Receptor specific IgG was purified by human Insulin Receptor affinity chromatograpy. Antibody characteristics include: Applications: This Insulin Receptor Polyclonal Antibody, has been validated for use in Western blot, ELISA, and immunohistochemistry Host species and isotype: This is a rabbi...
Catalog: 11081-RP02-AB
Size: 100 µg
Size: 100 µg
This is a purified rabbit anti-human Transferrin Receptor Polyclonal Antibody, that can be used in the validated applications of Western blot, ELISA, and immunohistochemistry. This Transferrin Receptor Polyclonal Antibody was produced in rabbits immunized with purified, recombinant human Transferrin Receptor. Transferrin Receptor specific IgG was purified by human Transferrin Receptor affinity chromatograpy. Antibody characteristics include: Applications: This Transferrin Receptor Polyclonal Antibody, has been validated for use in Western blot, ELISA, and immunohistochemistry Host species and ...
Catalog: 11020-RP02-AB
Size: 100 µg
Size: 100 µg
Sodium Pyruvate is commonly added to cell culture medium as an energy source in addition to glucose. Since the cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not necessary to supplement all cultures. However, if the cells have been grown in medium supplemented with sodium pyruvate, we recommend the supplementation is continued as the cells become accustomed to this addition and their growth will lag without it. Gibco® Sodium Pyruvate, 100 mM Solution is formulated as 11 g per liter of water. The final concentration of sodium pyruvate used in most cell...
Catalog: 11360-070
Size: 100 ml
Size: 100 ml
Gibco® MEM Vitamin Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Vitamin Solution is formulated to contain 100x the vitamins found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Vitamin Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001...
Catalog: 11120-052
Size: 100 ml
Size: 100 ml
7.5 g/100 ml Prepared with Bovine Serum Albumin Fraction V in phosphate-buffered saline.
Catalog: 15260-037
Size: 100 ml
Size: 100 ml
The protein concentration of each lot of Bovine Pituitary Extract is determined by the Bradford method. Each vial of BPE contains not less than 25 mg of protein.
Catalog: 13028-014
Size: 25 mg
Size: 25 mg
Gibco® MEM Amino Acids Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Amino Acids Solution is formulated to contain 50X the essential amino acids (except L-glutamine) found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Amino Acids Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufactur...
Catalog: 11130-051
Size: 100 ml
Size: 100 ml
Knockout™ Serum Replacement (Knockout™ SR) is a defined, serum-free formulation used to support the growth of undifferentiated ES (Embryonic Stem Cells) and iPS (Induced Pluripotent Stem Cells) in culture. It directly replaces FBS in existing protocols. One of the most important parameters surrounding ES cell culture is the maintenance of ES cells in an undifferentiated state. Until now, FBS was the only option for nutritive supplementation of ES and iPS cell media. Unfortunately, FBS also contains factors which can promote differentiation of ES cells. Thus, each lot must be screened prior to ...
Catalog: 10828010
Size: 100 ml
Size: 100 ml
Gibco® 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. Mercaptoethanol is not stable in solution so most protocols require daily supplementation. Gibco® 2-Mercaptoethanol is made at 50 mM in Dulbecco's Phosphate Buffered Saline (DPBS). Product Use For Research Use Only. Not for use in diagnostic procedures. cGMP Manufacturing and Quality System Gibco® 2-Mercaptoethanol is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the...
Catalog: 31350-010
Size: 20 ml
Size: 20 ml
The F4/80, clone BM8, Pacific Blue™ dye conjugate is a monoclonal rat antibody that is specific to mouse F4/80 and recognizes the expressed product of the Emr1 gene. Our Pacific Blue™ dye is optimally excited by the violet laser and conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis. Antibody Specifications Description: F4/80, Rat IgG2a monoclonal Label: Pacific Blue™ dye Validated Application: Flow Cytometry Spec...
Catalog: MF48028
Size: 1 ml
Size: 1 ml
The CD90/Thy-1 (clone G7) R-PE (phycoerythrin) conjugate is a monoclonal rat antibody that recognizes the Thy-1.1 and Thy-1.2 alloantigens (Thy-1 epitope region A). R-PE is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thereby affording exceptional quantum yields and molar extinction coefficients. The use of PE in antibody conjugates provides probes with greatly enhanced detectability. Antibody Specifications Description: CD90/Thy-1, Rat IgG 2c monoclonal Label: R-PE (phycoer...
Catalog: A14726
Size: 25 µg
Size: 25 µg
The CD45, clone HI30, PerCP-Cy ® 5.5 conjugate is a mouse monoclonal antibody (mAb) that recognizes human CD45 molecule. The PerCP-Cy ® 5.5 tandem conjugate combines the peridinin-chlorophyll proteins (PerCP) complexes, found in nature, with the Cy ® 5.5 cyanine dye; thereby permitting simultaneous multicolor labeling and detection of multiple targets by utilizing a single excitation source - the 488 nm line of an argon-ion laser. Antibody Specifications Description: CD45, Mouse IgG 1 monoclonal Label: PerCP-Cy ® 5.5 tandem dye Validated Application: Flow Cytometry Species Reactivity: Human Ex...
Catalog: A14796
Size: 25 µg
Size: 25 µg
Attune Insulin, Transferrin & Iron Supplements
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